Cuticular chemical profiles of Euglossa cordata L. males were analyzed to test whether ecological predictors affect their composition and relative proportion. Males were collected in areas of Caatinga and Atlantic Forest from Brazil during two distinct seasonal periods. We found 48 compounds from the cuticular extracts of males, which consisted of hydrocarbons (71.39%), acetates (16.79%), esters (10.5%), alcohols and others (1.31%). We verified that when specimens were separated between biomes, they did not show a qualitative differentiation, but a small quantitative variation of compounds was found between some alkanes. We suggest that these results reflect stability of epicuticular compounds even under variable environmental conditions.