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22 April 2021 Plant Volatiles and Oviposition Behavior in the Selection of Barley Cultivars by Wheat Stem Sawfly (Hymenoptera: Cephidae)
Buddhi B. Achhami, Gadi V. P. Reddy, M. L. Hofland, Jamie D. Sherman, Robert K. D. Peterson, David K. Weaver
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Abstract

Wheat stem sawfly, [Cephus cinctus (Hymenoptera: Cephidae)], females display complex behaviors for host selection and oviposition. Susceptible hollow stem wheat (Triticum aestivum L.) cultivars release a greater amount of attractive compound, (Z)-3-hexenyl acetate and receive a greater number of eggs compared to resistant solid stem wheat cultivars. However, barley (Hordeum vulgare L.) is becoming a more common host for C. cinctus in Montana. Therefore, how do host selection and oviposition behaviors on barley cultivars compare to what happens when encountering wheat cultivars? To answer this question, we carried out greenhouse experiments using two barley cultivars: ‘Hockett’ and ‘Craft’. Between these cultivars at Zadoks stages 34 and 49, we compared host selection decisions using a Y-tube olfactometer, compared oviposition behaviors on stems, and counted the number of eggs inside individual stems. In Y-tube bioassays, we found a greater number of C. cinctus females were attracted to the airstream passing over ‘Hockett’ than ‘Craft’ barley cultivars. Although the frequencies of oviposition behaviors were similar between these cultivars, the number of eggs was greater in ‘Hockett’. Volatile profiles indicated that the amount of linalool was greater in the airstream from ‘Craft’ than in ‘Hockett’ at Zadoks 34 while the amount of (Z)-3-hexenyl acetate was greater in airstream from ‘Hockett’ at both Zadoks 34 and 49. These results suggest that volatiles of barley plants influenced host selection behavior of ovipositing C. cinctus females, while other discriminating behaviors do not differ between cultivars.

© The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Buddhi B. Achhami, Gadi V. P. Reddy, M. L. Hofland, Jamie D. Sherman, Robert K. D. Peterson, and David K. Weaver "Plant Volatiles and Oviposition Behavior in the Selection of Barley Cultivars by Wheat Stem Sawfly (Hymenoptera: Cephidae)," Environmental Entomology 50(4), 940-947, (22 April 2021). https://doi.org/10.1093/ee/nvab035
Received: 15 January 2021; Accepted: 18 March 2021; Published: 22 April 2021
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KEYWORDS
(Z)-3-hexenyl acetate
host choice
linalool
Y-tube olfactometer
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