The results of a floristic study of lichens in Portage County, Wisconsin are presented. A total of 180 species in 73 genera were identified from field collections made in 2010 through 2013. A catalogue of species is included with 208 lichens presented as a result of field work, a literature search for previously reported collections and a physical search of the Wisconsin State Herbarium (WIS). There are 115 new records for the county and the following three species are reported for the first time for the State of Wisconsin: Leptogium rivulare, Heterodermia obscurata, and Peltigera extenuata. In addition to state and county records, two lichens of significant ecological interest were collected during this study; Lobaria pulmonaria, and Normandina pulchella.