We present a substantially revised and updated lichen flora for Denali National Park and Preserve (DNPP) based on a series of field investigations conducted from 2001 to 2011. During this period, we increased the number of lichen taxa recorded in DNPP by 175 species through new collections made at 46 inventory sites and 44 vegetation monitoring study areas within the 2.4 million ha DNPP. The revised checklist we present here includes 459 taxa (in 158 genera) that occur in DNPP. We discuss the major lichen community composition patterns in this vast and previously under-studied region, and provide notes for 24 of the most noteworthy taxa discovered during our fieldwork, including geographic and ecological summaries. We identify remaining knowledge gaps and needs for future lichen inventory work in DNPP, including surveys for microlichens.