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1 December 2018 Toxicity of Five Plant Oils to Adult Tribolium castaneum (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae)
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Abstract

Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) cause extensive damage to many stored products, thereby reducing their nutritional and economic value. However, controlling these pests with insecticides can have an impact on the environment as well as on human health. Thus, it is important to identify more environmentally safe compounds, such as plant oils, that can be used as an alternative means of pest control. In this study, we investigated the toxicity of 5 plant oils isolated from lavender (Lavandula angustifolia Mill., Lamiaceae), onion (Allium cepa L., Amaryllidaceae), flax (Linum usitatissimum Mill., Linaceae), caraway (Carum carvi [Lindl.] H. Wolff, Apiaceae), and brown galingale (Cyperus fuscus, or saad L., Cyperaceae) for use on the stored-products insect pests T. castaneum and O. surinamensis. The efficacy of these oils was evaluated at concentrations of 1, 2, 3, and 4 µL per mL and at an exposure time of 24 h. Results indicated that O. surinamensis was more susceptible to plant oils than T. castaneum, which showed a greater resistance to these natural products. Results also revealed that caraway and lavender oils were the most toxic of all treatments rendered for T. castaneum, with 50% lethal concentration (LT50) values of 1.2 and 2.4 µL per mL, respectively. The onion and lavender oils displayed the highest efficacy on O. surinamensis with insect mortality reaching 100% with onion oil and an LT50 value for lavender oil of 0.6 µL per mL. These results suggest that onion, lavender, and caraway oils are potentially promising and environmentally acceptable alternatives for the control of T. castaneum and O. surinamensis.

Agricultural crops are the most important food source for millions of people worldwide. However, in most cases, grains are not used immediately after harvesting, but instead are stored for the next season or exported to other countries. The loss of grain yield during storage can be attributed to many factors, and among those, insect pests are the most important cause of loss because they reduce the quality of the stored grain (Jayakumar et al. 2017).

Unchecked growth of human population has led to numerous and very serious problems, including food shortage. Globally, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) are the 2 most important stored–grain pests, damaging 10 to 40% of all stored agricultural crops (Al Qahtani et al. 2012). These beetles feed on grains, dried fruits, flour, sugar, candies, tobacco, and many other plant products intended for human consumption, as well as dried meat (Bilal et al. 2015).

Currently, the intensive use of chemicals for the control of storedproducts pests has resulted in serious problems, including pest resistance to insecticides, environmental contamination, the presence of unacceptable pesticide residues in foods, and lethal effects on nontarget organisms (White & Leesch 1995; Jovanovic et al. 2007; Lu et al. 2011). Botanical insecticides have long been used as alternatives to synthetic insecticides for insect pest management because botanicals pose little threat to the environment or to human health (Isman 2006; Ahmad et al. 2013). Many previous studies have demonstrated the effectiveness of plant oils to control stored–products insects. Magd El–Din (2001) evaluated the efficacy of 3 essential oils to control 3 storedproduct insects, among which caraway seed oil demonstrated high toxicity to both insect eggs and adults. Plant oils comprise complex mixtures of monoterpenes, sesquiterpenes, and aromatic compounds (Nattudri et al. 2017), and may act as contact insecticides, antifeedants, repellents, or fumigants (Jayakumar et al. 2017).

Essential oils are interesting natural plant products thatpossess various biological activities, among other qualities. These materials rapidly degrade under ambient air temperature and moisture, and are readily broken down by detoxification enzymes. These features are important for their use as biopesticides, because a rapid breakdown means less persistence in the environment (i.e., lower residual effects), and reduced risks to non–target organisms. Although natural enemies are sensitive to direct contact with such materials, predators and parasitoids that attack the product 1 to 2 d after treatment application are not affected by the toxins (Isman 2006).

In this study, we evaluated the effectiveness of 5 types of plant oils on T. castaneum and O. surinamensis. These plant oils were selected based on prior knowledge regarding their effective toxicity to control similar pests (Magd–El–Din 2001; Al–Jabr 2006; Lopez et al. 2008; Germinara et al. 2017). These oils also are relatively inexpensive and readily available in most countries.

Materials and Methods

PLANT OILS

Pure plant oils were obtained from Diar Almadina Company in Jeddah, Saudi Arabia (Table 1). We evaluated 4 concentrations of these oils (1, 2, 3, and 4 µL per mL) diluted in acetone, that was used as a solvent because of its rapid evaporation.

INSECT REARING TECHNIQUE

Tribolium castaneum and O. surinamensis were collected from infested products obtained from a local market in Albaha City, Saudi Arabia, and reared under laboratory conditions at 27 °C, 70 ± 5% relative humidity, and 12:12 h (L:D) photoperiod. Yeast and flour (5:100 g) were used as food for the insects that were maintained in jars covered with muslin cloth fastened with rubber bands. One– to 3–wk–old adults were collected and used for bioassays (Madkour et al. 2013).

CONTACT TOXICITY BIOASSAY

As a bioassay, we used the thin film technique described by Iwuala et al. (1981). One mL of each concentration of the plant oils was evenly spread over the bottom of a Petri dish. Petri dishes used for control treatments were treated with 1 mL of acetone. Mature adults were exposed to a thin film of plant oils for 24 h, when mortality was recorded following adjustment with Abbott's (1925) formula. Four replicates of 10 adults were used for each concentration treatment.

Table 1.

Plant oils used in bioassays and source of essential oil.

t01_592.gif

Statistical analysis

The data were analyzed using 1–way analysis of variance (ANOVA), followed by Tukey's honest significant difference (HSD) test. Differences were considered significant at P < 0.05. The LC50 values (oil concentration high enough to kill 50% of the insects) were calculated using Probit analysis (Finney 1964).

Results

The insecticidal activity of lavender, onion, flaxseed, caraway, and saad oils tested on T. castaneum are listed in Table 2 and depicted in Figures 1 to 5, respectively. The data indicated that caraway oil was the most toxic of the oils evaluated, causing a mortality rate of 50% at the lowest concentration (1 µL per mL) and 95% mortality at the highest concentration (4 µL per mL), with LT50 value of 1.2 µL per mL. For insects treated with lavender oil for 24 h, mortality was 30% at a concentration of 1 µL per mL and 70% at 4 µL per mL. Onion oil was less potent than the 2 aforementioned oils, causing only 5% mortality at a concentration of 1 µL per mL and 90% mortality at 4 µL per mL. Flaxseed and saad oils showed similarly low toxic effects, with LT50 values of 4.5 and 4.24 µL per mL, respectively. Results clearly indicated that insect mortality increased with increasing oil concentration. Thus, plant oils can be ranked as follows according to their toxicity to control T. casteneum: caraway > lavender > onion > saad > flaxseed.

Insecticidal activity of the 5 plant oils examined on O. surinamensis are listed in Table 3, and those of lavender, flaxseed, caraway, and saad oils are depicted in Figures 6 to 9, respectively. Onion oil caused 100% insect mortality at all concentrations that were examined. The LT50 value was 0.6 µL per mL for lavender oil, which caused 65% mortality at a concentration of 1 µL per mL and 90% mortality at 4 µL per mL, after a 24 h exposure. Insects treated with saad oil exhibited 60% mortality at a concentration of 1 µL per mL and 90% mortality at 4 µL per mL, with an LT50 value of 0.77 µL per mL. Caraway oil caused 100% insect mortality at the highest concentration, with an LT50 value 0.81 µL per mL. Flaxseed oil caused 55% and 95% mortality in insects treated at 1 and 4 µL per mL, respectively, and produced an LT50 value of 1 µL per mL. Based on these results, plant oils were ranked in the following order according to their toxicity for O. surinamensis: onion > lavender > saad > caraway > flaxseed.

Discussion

The results of this study demonstrated that the toxicity of the 5 selected plant oils varied depending on the oil concentration and target insect species. Caraway, lavender, and onion oils were most effective in controlling T. castaneum and O. surinamensis. These findings are consistent with the results of previous studies that have investigated the toxic effects of plant oils on these 2 insects (Al–Jabr 2006; Mondal & Khalequzzaman 2006; Ahmed et al. 2009; Lu et al. 2011; Hamed et al. 2012; Madkour et al. 2013; Iieke & Ogungbite 2014; Aref & Farashiani 2015; Khanzada et al. 2015). These studies indicated that edible plant oils affect the developmental stages of T. castaneum, resulting in low adult emergence and pest fecundity in grain treated with plant oils.

Table 2.

Mortality of Tribolium castaneum adults exposed to different concentrations of plant oils.

t02_592.gif

Fig. 1.

Log concentration–mortality regression line for the activity of lavender oil on Tribolium castaneum.

f01_592.jpg

Fig. 2.

Log concentration–mortality regression line for the activity of onion oil on Tribolium castaneum.

f02_592.jpg

Fig. 3.

Log concentration–mortality regression line for the activity of flaxseed oil on Tribolium castaneum.

f03_592.jpg

Fig. 4.

Log concentration–mortality regression line for the activity of caraway oil on Tribolium castaneum.

f04_592.jpg

Fig. 5.

Log concentration–mortality regression line for the activity of saad oil on Tribolium castaneum.

f05_592.jpg

Talukder et al. (1998) demonstrated that the toxic effects of plant oils are due to the presence of oleic, linoleic, and palmitic acids, and alkalis that may block insect tracheae, thereby inhibiting respiration during contact or fumigation tests. Yoon et al. (2011) showed a repellent effect of lavender oil for the spotted lantern fly, Lycorma delicatula (White) (Hemiptera: Fulgoridae) (also referred to as “spot clothing wax cicada” or “Chinese blistering cicada” in the literature) and established that linalool (a monoterpene) was the most effective constituent present in this oil. Similarly, Lucic et al. (2015) demonstrated the toxic effects of the lavender plant parts and essential oil on 3 stored–wheat pests, including T. castaneum. Other studies have concluded that plant essential oils from the family Linaceae, including lavender oil, may cause feeding inhibition, repellence, and insecticidal action to control various insect pests (Papachristos & Stamopoulus 2004; Gonzalez– Coloma et al. 2006). Furthermore, Magd–El–Din (2001) demonstrated that caraway oil exhibited a high toxicity for Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and other pests. Fang et al. (2010) found that the essential oil of C. carvi showed a strong toxicity in controlling Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) and T. castaneum with (R)–carvone and D–limonene being the principal toxic constituents for these 2 insects.

Table 3.

Mortality of Oryzaephilus surinamensis adults exposed to different concentrations of plant oils.

t03_592.gif

Fig. 6.

Log concentration–mortality regression line for the activity of lavender oil on Oryzaephilus surinamensis.

f06_592.jpg

Fig. 7.

Log concentration–mortality regression line for the activity of flaxseed oil on Oryzaephilus surinamensis.

f07_592.jpg

Fig. 8.

Log concentration–mortality regression line for the activity of caraway oil on Oryzaephilus surinamensis.

f08_592.jpg

Fig. 9.

Log concentration–mortality regression line for the activity of saad oil on Oryzaephilus surinamensis.

f09_592.jpg

Several studies have demonstrated that plant extracts can be more effective in controlling pest insects than individual active compounds, owing to natural synergism, that also may delay the development of insecticide resistance (Yoon et al. 2011; Bilal et al. 2015). In this study, O. surinamensis was found to be more susceptible to the toxic effects of the oils, whereas T. castaneum was more resistant, as previously reported by Al–Jabr (2006). Interestingly, in the present study we found that A. cepa oil was highly toxic to O. surinamensis; however, other studies that investigated the effect of A. cepa oil on insect pests did not report similar results (Tunaz et al. 2009; Denloye 2010).

In conclusion, the results of the present study indicate that onion, lavender, and caraway oils are promising alternatives for the control of T. castaneum and O. surinamensis in stored products. Synthetic insecticides cause adverse effects on the environment, including pest resistance, pollution, and toxicity to non–target organisms. Plant essential oils are less likely to elicit pest resistance and are less toxic to the environment. However, further studies are needed to evaluate the cost of these oils and their effectiveness in controlling a wider range of insect pests.

Acknowledgments

The authors would like to thank Fatimah Alzahrani for her assistance during the preparation of the manuscript.

References Cited

1.

Abbott W. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265–267. Google Scholar

2.

Ahmed S, Zainab A, Nisar S, Rana N. 2009. Effect of new formulations of neem products on biology of Tribolium castaneum (Herbst) (Tenebrionidae: Coleoptera). Pakistan Entomologist 31: 133–137. Google Scholar

3.

Al–Jabr AM. 2006. Toxicity and repellency of seven plant essential oils to Oryzaephilus surinamensis (Coleoptera: Silvanidae) and Tribolium castaneum (Coleoptera: Tenebrionidae). Scientific Journal of King Faisal University 7: 49–59. Google Scholar

4.

Al Qahtani AM, Al–Dahafar ZM, Rady M. 2012. Insecticidal and biochemical effect of some dried plants against Oryzaephilus surinamensis (Coleoptera: Silvanidae). Journal of Basic and Applied Zoology 65: 88–93. Google Scholar

5.

Aref PS, Farashiani EM. 2015. Eucalyptus dundasii Maiden essential oil, chemical composition and insecticidal values against Rhyzopertha dominica (F.) and Oryzaephilplus surinamensis (L.). Journal of Plant Protection Research 55: 35–41. Google Scholar

6.

Bilal H, Akram W, Hassan SA, Zia A, Bhatti AR, Mastoi I, Aslam S. 2015. Insecticidal and repellent potential of citrus essential oils against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Pakistan Journal of Zoology 47: 997–1002. Google Scholar

7.

Denloye A. 2010. Bioactivity of powder and extracts from garlic, Allium sativum L. (Alliaceae) and spring onion, Allium fistulosum (Alliaceae) against Callosobruchus macullatus F. (Coleoptera: Bruchidae) on cowpea, Vigna unguiculate (L.) Walp (Leguminosae) seeds. Psyche 2010: 1–5.  http://dx.doi.org/10.1155/2010/958348  Google Scholar

8.

Fang R, Jiang CH, Wang XY, Zhang HM, Liu ZL, Zhou L, Du SS, Deng ZW. 2010. Insecticidal activity of essential oil of Carum carvi fruits from China and its main components against two grain storage insects. Molecules 15: 9391–9402. Google Scholar

9.

Finney DJ. 1964. Probit Analysis. 2nd ed. Cambridge University Press, Cambridge, United Kingdom. Google Scholar

10.

Germinara G, Stefano M, Acutis L, Pati S, Delfine S, Cristofaro A, Rotundo G. 2017. Bioactivities of Lavandula angustifolia essential oil against the stored grain pest Sitophilus granarius. Bulletin of Insectology 70: 129–138. Google Scholar

11.

Gonzalez–Coloma A, Martin–Benito D, Mohamed N, Garcia–Vallejo V, Soria A. 2006. Antifeedant effects and chemical composition of essential oils from different populations of Lavandula luisieri L. Biochemical Systematics and Ecology 34: 609–616. Google Scholar

12.

Hamed KR, Abotaleb MS, Bahria B. 2012. Efficacy of certain plant oils as grain protectants against the rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) on wheat. Egyptian Academic Journal of Biological Sciences 5: 49–53. Google Scholar

13.

Iieke DK, Ogungbite OC. 2014. Entomocidal activity of powders and extracts of four medicinal plants against Sitophilus oryzae (L), Oryzaephilus surinamensis Mercator (Faur) and Ryzopertha dominica (Fabr). Jordan Journal of Biological Sciences 7: 57–62. Google Scholar

14.

Isman MB. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51: 45–66. Google Scholar

15.

Iwuala MOE, Ossiogu IUW, Agbackwuru EOP. 1981. Dennettia oil, a potential insecticide: tested with adults and nymphs of Periplaneta americana and Zonocerus variegatus. Journal of Economic Entomology 74: 249–252. Google Scholar

16.

Jayakumar M, Arivoli S, Raveen R, Tennyson S. 2017. Repellent activity and fumigant toxicity of a few plant oils against the adult rice weevil Sitophilus oryzae Linnaeus 1763 (Coleoptera: Curculionidae). Journal of Entomology and Zoology Studies 5: 324–335. Google Scholar

17.

Jovanovic Z, Kostic M, Popovic Z. 2007. Grain–protective properties of herbal extracts against the bean weevil Acanthoscelides obtectus Say. Industrial Crops and Products 26: 100–104. Google Scholar

18.

Khan FZA, Sagheer M, Hasan M, Saeed S, Ali K, Gul HT, Bukhar SA, Manzoor SA. 2013. Toxicological and repellent potential of some plant extracts against stored product insect pest, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae). International Journal of Biosciences 3: 280–286. Google Scholar

19.

Khanzada H, Sarwar M, Lohar M. 2015. Repellence activity of plant oils against red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) in wheat. International Journal of Animal Biology 1: 86–92. Google Scholar

20.

Lopez M, Jordan M, Pascual–Villalobos M. 2008. Toxic compounds in essential oils of coriander, caraway and basil active against stored rice pests. Journal of Stored Products Research 44: 273–278. Google Scholar

21.

Lu J, Wu C, Shi Y. 2011. Toxicity of essential oil from Artemisia argyi against Oryzaephilus surinamensis (Linnaeus) (Coleoptera: Silvanidae). African Journal of Microbiology Research 5: 2816–2819. Google Scholar

22.

Lucic P, Liska A, Rosman V, Balicevic R, Dumlic M. 2015. The potential use of lavender (Lavandula intermedia) in protection of stored wheat against storage insect, pp. 160–165 In Proceedings of the 8th International Scientific/ Professional Conference Agriculture in Nature and Environment Protection. Vukovar, Croatia, 1–3 Jun 2015 . Google Scholar

23.

Madkour HM, Zaitoun AA, Singer F. 2013. Repellent and toxicity of crude plant extracts on saw–toothed grain beetle (Oryzaephilus surinamensis) (L.). Journal of Food, Agriculture and Environment 11: 381–384. Google Scholar

24.

Magd–El–Din M. 2001. Biological activity of three essential oils against some stored product insects (Coleoptera: Bostrichidae, Curclionidae, and Tenebrionidae). Journal of the Egyptian–German Society of Zoology 34(E): 143–155. Google Scholar

25.

Mondal M, Khalequzzaman M. 2006. Toxicity of essential oils against red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Journal of Bio–Science 14: 43–48. Google Scholar

26.

Nattudri G, Arulvasu C, Baskar K. 2017. Indigenous knowledge in stored product pest management. Entomology, Ornithology and Herpetology 6: 2. DOI: 10.4172/2161–0983.1000e127 Google Scholar

27.

Papachristos DP, Stamopoulos DC. 2004. Fumigant toxicity of three essential oils on the eggs of Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Products Research 40: 517–525. Google Scholar

28.

Talukder D, Malek MA, Khanam M, Dey KC. 1998. Toxicity of some indigenous plant seed oils against Tribolium confusum Duval adults (Coleoptera: Tenebrionidae). Pakistan Journal of Zoology 30: 331–334. Google Scholar

29.

Tunaz H, Kubilay M, Isikber AA. 2009. Fumigant toxicity of plant essential oils and selected monoterpenoid components against the adult German cockroach, Blattella germanica (L.) (Dictyoptera: Blattellidae). Turkish Journal of Agriculture and Forestry 33: 211–217. Google Scholar

30.

White NDG, Leesch JG. 1995. Chemical control, pp. 287–330 In Subramanyam B, Hagstrum DW [eds.], Integrated Management of Insects in Stored Products. Marcel Dekker, New York, USA. Google Scholar

31.

Yoon C, Moon SR, Jeong J, Shin Y, Cho S, Ahn K, Yang J, Kim GH. 2011. Repellency of lavender oil and linalool against spot clothing wax cicada, Lycorma delicatula (Hemiptera: Fulgoridae) and their electrophysiological responses. Journal of Asia–Pacific Entomology 14: 411–416 Google Scholar
Fatehia Gharsan, Nihad Jubara, Lamya Alghamdi, Zahraa Almakady, and Eisha Basndwh "Toxicity of Five Plant Oils to Adult Tribolium castaneum (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae)," Florida Entomologist 101(4), 592-596, (1 December 2018). https://doi.org/10.1653/024.101.0420
Published: 1 December 2018
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