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11 April 2019 A New Host Record for Euphoria spp. Burmeister (Coleoptera: Melolonthidae: Cetoniinae) in Oaxaca, Mexico: Tagetes patula (Asteraceae)
Julián Hernández-Cruz, Héctor Miguel Guzmán-Vásquez, Rafael Pérez-Pacheco, Carlos Alejandro Granados-Echegoyen
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A total of 48 specimens of Euphoria (Coleoptera: Cetoniinae) were collected, represented by a single specimen of E. biguttata, and 47 specimens E. subtomentosa. We report for the first time these two beetles (Melolonthidae) feeding on flowers of Tagetes patula (Asteraceae). Additionally, this is the first time the species were observed in Sola de Vega, Oaxaca State, Mexico.

Tagetes patula Linnaeus (Asteraceae), popularly known as dwarf marigold or French marigold, is an annual plant, native to North America and widely disseminated throughout the world. The genus Euphoria Burmeister (Coleoptera: Scarabaeidae: Cetoniinae) includes 59 species, with Mexico having the greatest biodiversity (40 species). These species are distributed from southern Canada down to northern Argentina, although the highest specific richness is found from the southern U.S. to Guatemala in very diverse environments, principally above 1,000 masl. There is little documented information about the life cycle, feeding habits, and phenology of the 59 species of these Cetoniinae, and what is published on this insect is often imprecise (Orozco 2012). It is commonly known that larvae are found in soils with high levels of organic matter or sandy soils, under dry manure, and mounds of ant detritus (Deloya 1988). Little is known about the rhizophagous feeding habits of the larval stage of this insect, and only the dark-flower scarab Euphoria sepulcralis (F.) (Coleoptera: Cetoniidae) has been reported with this feeding habit (Buss 2004). The adults of the insect usually feed on liquid exudates (extrafloral nectaries) and tender parts of plants, and manure. The feeding habits of this insect on plant flowers is not extensively documented. The plants with large or abundant aerial parts are preferred as hosts by adults of the genus Euphoria, usually plants in the family Asteraceae. These plants provide glucose exudates (extrafloral nectaries) for the feeding of these beetles (Orozco 2012).

The specimens were collected manually in a T. patula culture area in the experimental campus of the Universidad Tecnologica de la Sierra Sur de Oaxaca, located in the municipality of Villa Sola de Vega in Oaxaca State, Mexico (16.5116°N, 96.9794°W, 1,440 masl, Fig. 1) from 5 to 8 Oct 2017. This plant is known in the region as Flor de San Miguelito, and occurs widely in relict pine-oak forest, agave, and maize crops, and in the presence of xeric scrubs. These specimens were identified as Euphoria biguttata (Gory & Percheron) (a single specimen) and Euphoria subtomentosa (Gory & Percheron) (47 specimens) (both Coleoptera: Cetoniidae). They were identified by using the key in Orozco (2012). The specimens were deposited in the collection of Ph.D. J. Hernández-Cruz in the Laboratory of Entomology of the Universidad Tecnologica de la Sierra Sur de Oaxaca.

Tagetes patula is documented for the first time as a host of these beetles. Euphoria biguttata has been observed on Helianthus spp. and Tithonia tubiformis (Jacq.) (both Asteraceae) (Orozco 2012). Morón et al (1997) documented adults feeding on flowers of Mimosa sp. and Calliandra sp. Benth. (both Fabaceae) in the following localities from Oaxaca State, Mexico: Huajuapan de León, Santiago Cacaloxtepec, Santiago Chazumba (Mixteca Region), and San Pedro Totolapan (Central Valleys Region) at altitudes above 1,000 masl.

The adults of E. subtomentosa also have been observed in flowers of the family Asteraceae: Bidens odorata Cav., Dyssodia pinnata (Cav.) B. L. Rob., and Helianthus annuus L. (Orozco 2012), although they also have been collected in detritus of Atta mexicana (Smith) (Hymenoptera: Formicidae) (Deloya 1988). The localities where this species has been recorded in Oaxaca State are: Huajuapan de León, San Francisco Huapanapan, Asunción Nochixtlán, Santiago Cacaloxtepec, Santiago Chazumba (Mixteca Region), Monte Albán, Oaxaca-Centro, San Pablo Villa de Mitla, San Sebastián Tutla (Central Valleys Region), and San Juan Bautista Cuicatlán (Cañada Region).

Orozco (2012) mentioned that E. sepulcralis is associated as an insect-host of 59 plant families, including the genus Tagetes spp., but the species E. biguttata and E. subtomentosa, reported here, are the only species of this insect genus that have been found feeding in flowers T. patula (Fig. 2).

The genus Tagetes has many biological properties that affect organisms, including fungi (Romagnoli et al. 2005), bacteria (Dasgupta et al. 2012), nematodes (Macedo et al. 2012), and insects (Faizi et al. 2011). A large number of studies have reported the biocidal properties of extracts obtained from different species of Tagetes. This plant genus contains secondary metabolites (in greater quantity in the aerial parts) that function as toxins, repellents, or growth regulators (Díaz-Cedillo & Serrato-Cruz 2011) against some insect pests. Phytochemical studies with flowers of T. patula identified terpenes (Prakash et al. 2012), alkaloids (Faizi & Naz 2002), thiophenes (Szarka et al. 2006), and flavonoids (Guinot et al. 2008) responsible for a variety of biocide properties (Massera et al. 1998).

We report evidence of an organism (Insecta: Coleoptera: Melolonthidae) with potential to be an insect pest by feeding on a plant with insecticidal properties, suggesting that beetles of the genus Euphoria may have developed resistance to the secondary metabolites produced by Tagetes spp.

Fig. 1.

Map of the state of Oaxaca, Mexico, showing the locations of collection records of Euphoria subtomentosa and Euphoria biguttata.


Plant secondary metabolites have multiple functions throughout the plant life cycle, affecting the interaction of plants with their environment, including the relationship of plants with pollinators, herbivores, and predators of herbivores (Dixon 2001). Resistance to antagonist organisms in plants has been identified as one of the major defense functions, but a large diversity of abiotic and biotic environmental factors are found to significantly affect the production of plant secondary metabolites, factors that could affect its function as toxins, and interfere with insect host search patterns (Kessler 2015).

After consulting the literature, we concluded that this is the first time that E. biguttata and E. subtomentosa were reported to feed on flowers of Tagetes patula (Asteraceae). Additionally, this is the first time the species were observed in Sola de Vega, Oaxaca State, Mexico.

We are grateful to the students of “Agricultura Sustentable y Protegida” career of the Universidad Tecnologica de la Sierra Sur de Oaxaca, Araceli Amador-Reyes, Zenaido Martínez-Merino, Sandra P. Martínez-Rafael, and Javier Sandoval-Sandoval, who collaborated in the manual collection of the specimens.

Fig. 2.

Photographs of Euphoria specimens and blossoms: A, B = Tagetes patula flowers; C = Euphoria biguttata dorsal view; D = Euphoria subtomentosa dorsal view.


References Cited


Buss EA. 2004. New host record for Euphoria sepulcralis (Coleoptera: Scarabaeidae). The Coleopterists' Bulletin 58: 328–328. Google Scholar


Dasgupta N, Ranjan S, Saha P, Jain R, Malhotra S, Saleh AM. 2012. Antibacterial activity of leaf extract of Mexican marigold (Tagetes erecta) against different gram positive and gram negative bacterial strains. Journal of Pharmacy Research 5: 4201–4203. Google Scholar


Deloya C. 1988. Los Coleópteros lamelicornios asociados a depósitos de detritos de Atta mexicana (Smith) (Hymenoptera: Formicidae) en el Sur de estado de Morelos. Folia Entomológica Mexicana 75: 77–91. Google Scholar


Díaz-Cedillo F, Serrato-Cruz M. 2011. Composición esencial de Tagetes parryi A. Gray. Revista Fitotecnia Mexicana 34: 145–148. Google Scholar


Dixon RA. 2001. Natural products and plant disease resistance. Nature 411: 843–847. Google Scholar


Faizi S, Fayyaz S, Bano S, Iqbal EY, Lubna D, Siddiqi H, Naz A. 2011. Isolation of nematicidal compounds from Tagetes patula L. yellow flowers: structureactivity relationship studies against cyst nematode Heterodera zeae infective stage larvae. Journal of Agricultural and Food Chemistry 59: 9080–9093. Google Scholar


Faizi S, Naz A. 2002. Jafrine, a novel and labile E-carboline alkaloid from the flowers of Tagetes patula. Tetrahedron 58: 6185–6197. Google Scholar


Guinot P, Gargadennec A, Valette G, Fruchier A, Andary C. 2008. Primary flavonoids in marigold dye: extraction, structure and involvement in the dyeing process. Phytochemical Analysis 19: 46–51. Google Scholar


Kessler A. 2015. The information landscape of plant constitutive and induced secondary metabolite production. Current Opinion in Insect Science 8: 47–53. Google Scholar


Macedo TF, Bevilaqua CML, Oliveira LMB, Camurça-Vasconcelos ALF, Morais SM, Machado LKA, Ribeiro WL. 2012. In vitro activity of Lantana camara, Alpinia zerumbet, Mentha villosa and Tagetes minuta decoctions on Haemonchus contortus eggs and larvae. Veterinary Parasitology 190: 504–509. Google Scholar


Massera PE, Rodriguez T, Giulietti AM. 1998. Thiophene production in transformed roots cultures of Tagetes filifolia. Biotechnology Letters 20: 573–577. Google Scholar


Morón MA, Ratcliffe BC, Deloya C. 1997. Atlas de los Escarabajos de México (Coleoptera: Lamellicornia). Vol. 1. Familia Melolonthidae. CONABIO y Sociedad Mexicana de Entomología, México Distrito Federal, Mexico. Google Scholar


Orozco J. 2012. Monographic revision of the American genus Euphoria Burmeister, 1842 (Coleoptera: Scarabaeidae: Cetoniinae). The Coleopterists' Bulletin 66: 1–182. Google Scholar


Prakash O, Rout PK, Chanotiya CS, Misra LN. 2012. Composition of essential oil, concrete, absolute and SPME analysis of Tagetes patula capitula. Industrial Crops and Products 37: 195–199. Google Scholar


Romagnoli C, Bruni R, Andreotti E, Rai MK, Viventi CB, Mares D. 2005. Chemical characterization and antifungal activity of essential oil of capitula from wild Indian Tagetes patula L. Protoplasma 225: 57–65. Google Scholar


Szarka SZ, Hethelyi E, Lemberkovics E, Kuzovkina IN, Banyai P, Szoke E. 2006. GC and GC-MS studies on the essential oil and thiophenes from Tagetes patula L. Chromatographia 63: S67–S73. Google Scholar
Julián Hernández-Cruz, Héctor Miguel Guzmán-Vásquez, Rafael Pérez-Pacheco, and Carlos Alejandro Granados-Echegoyen "A New Host Record for Euphoria spp. Burmeister (Coleoptera: Melolonthidae: Cetoniinae) in Oaxaca, Mexico: Tagetes patula (Asteraceae)," Florida Entomologist 102(1), 236-239, (11 April 2019).
Published: 11 April 2019

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