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Marjorie A. Hoy, A. Jeyaprakash, Dionne Clarke-Harris
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The citrus leafminer Phyllocnistis citrella Stainton (CLM) is endemic to India, China and other Asian countries (Clausen 1931), but has spread rapidly into other citrus-growing areas throughout the world during the past 15 years. The CLM was first noticed in Florida in 1993 (Heppner 1993) and spread throughout the Caribbean, as well as Central and South America. A classical biological control project was initiated and the host-specific endoparasitoid Ageniaspis citricola Logvinovskaya (Hymenoptera: Encyrtidae) was imported and released in Florida (Hoy & Nguyen 1994, 1997). The Australian population of A. citricola had been imported from Thailand into Australia (Neale et al. 1995) prior to being released into Florida. Subsequently, another A. citricola population from Taiwan was imported and introduced into Florida (Lo & Chiu 1988; Hoy et al. 2000). Individuals from Taiwan and Thailand appear morphologically identical, but slight differences in biology and behavior were noted (Ujiye et al. 1996; Yoder & Hoy 1998), which led to molecular studies to characterize the two populations (Hoy et al. 2000; Alvarez & Hoy 2002). Hoy et al. (2000) showed, with RAPD-PCR and DNA sequence analysis of Actin1 and Actin2 genes, that there are likely 2 cryptic species of Ageniaspis parasitizing the CLM, 1 from Taiwan and 1 from Thailand. Both populations were released in Florida, and current, but limited, evidence suggests that only the population originating from Thailand established (Alvarez 2000).

While monitoring populations of the CLM in Jamaican citrus groves between 24 and 29 May 2004, high rates of parasitism (ranging from 30-90%) of the CLM by Ageniaspis were found throughout the island. To further evaluate the distribution and abundance of A. citricola in Jamaica, a survey was conducted between Jan and Sep 2005 at 4 sites in the citrus-growing regions of Jamaica. Tender new growth on citrus trees was monitored at these sites to determine the number of leafminer mines per leaf and to determine the proportion of pupal chambers that contained pupae of the citrus leafminer or A. citricola. A mean (±S.D.) of 41.3 (26.9)% of all pupal chambers examined contained A. citricola in these sites sampled during Jan-Sep 2005. On most sample dates when CLM was found, fewer than the targeted number of 40 pupal chambers could be sampled because leafminer densities were very low, averaging 0.50 (0.25) mines per leaf (Table 1). These survey data suggest that CLM populations are low in Jamaica and the establishment and abundance of A. citricola appears to be contributing to this status.

No purposeful releases of A. citricola were made in Jamaica and no one knows how this parasitoid managed to colonize the island. However, because Jamaican growers had never found CLM populations to cause serious damage to citrus, except for occasional population increases in nurseries and newly established trees (personal communication, Dr. Percy Miller, Citrus Growers Association, 2006), we speculate that A. citricola entered Jamaica with the citrus leafminer on citrus, and multiplied and spread throughout Jamaica. This conclusion was reached because the CLM is unable to develop on host plants other than citrus and that A. citricola is host specific to the CLM.

Adult parasitoids collected in 95% ethyl alcohol (EtOH) during May 2004 were taken to Gainesville, FL where they were analyzed to determine whether they were derived from the Taiwan or the Thailand populations of Ageniaspis by using Actin gene sequence data (Hoy et al. 2000). Genomic DNA from individual wasps was extracted by the PureGene method according to the manufacturer’s recommendations (Gentra, Minneapolis, MN) and resuspended in 10-μL of sterile water. High-fidelity PCR amplifications were performed by resuspending 1 μL of DNA in a 50-μL reaction containing 400 picomoles of degenerate primers (forward: MHO105, 20-mer, 5’TGGGAYGAYATGGARATHTGGCAYCA-3’; reverse: MHO99, 20-mer, 5’GCCATYTCYTGYTCRAARTC-3’, where Y = C or T, R = A or G, and H = A or C or T). The primers were expected to amplify a 400-bp region, 266 to 666 bp from the start site of the Actin gene family, which typically contains 6 different genes in insects. The primers were added to 50 mM Tris pH 9.2, 1.75 mM MgCl2, 16 mM ammonium sulfate, 350 μM dATP, dGTP, dCTP, dTTP and 0.2 units of Tgo and 1.0 units of Taq DNA polymerase (Roche Biochemicals, Indianapolis, IN). High-fidelity PCR amplification was performed for 35 cycles, with 3 linked profiles: (1) one cycle consisting of 94°C denaturation for 2 min; (2) 10 cycles consisting of 94°C denaturation for 10 s, 49°C annealing for 30 s and 68°C extension for 1 min; and (3) 25 cycles consisting of 94°C denaturation for 10 s, 49°C annealing for 30 s and 68°C extension for 1 min plus 20 s added for every consecutive cycle.

The 400-bp Actin1 gene sequences from the Australian and Taiwan populations show 20 base-pair differences between them (95% similarity), while the 400-bp Actin2 gene sequences show 12 base-pair differences (97% similarity) (Hoy et al. 2000). These Actin sequence differences are similar to the degree of sequence divergence found for other highly conserved genes and are equivalent to the differences found in Actin sequences from different genera. Hence, the Actin sequence differences observed between the Australian and Taiwan parasitoids indicate that they belong to different cryptic species.

Twelve clones were sequenced from the Jamaican Ageniaspis and 9 of the Actin sequences were identical to Actin1. Two clones produced sequences that were identical to Actin2 sequences from the Australian population of A. citricola, which was collected originally from Thailand. In addition, a third, and new, Actin sequence was found, with an 85% similarity to the Actin1 sequence of the Australian population. Because insects have 6 Actin genes, this new sequence will be called Actin3 and will be deposited in GenBank (Accession Number EF149011).


These data indicate that the population of A. citricola in Jamaica probably originated from the Australian (Thailand), rather than from the Taiwan, population. This is consistent with what is currently known about the origin of the established Ageniaspis population in Florida (Alvarez 2000). It is not known when, or how, A. citricola arrived in Jamaica, although the CLM was detected there in 1994. The fortuitous establishment of A. citricolaon the CLM in Jamaica is not the only such establishment of a natural enemy discovered during this 2004 survey of citrus. The parasitoid Lipolexis oregmae Gahan (Hymenoptera: Aphidiidae) was found attacking the brown citrus aphid, Toxoptera citricida Kirkaldy (Hemiptera: Aphididae) (Hoy et al., unpublished data), and the eulophid parasitoid Tamarixia radiata Waterston was found attacking the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). The fact that 3 natural enemies of 3 invasive citrus pests were found in Jamaica, none of which were purposefully imported and released, suggests that pest-infested citrus trees were imported into Jamaica without going through appropriate quarantine procedures. Because each pest arrived at different times, the parasitoids probably arrived at different times, as well. This indicates that an analysis is needed to identify the critical control points within those services in Jamaica that support border protection, and that procedures may require strengthening.

References Cited


J. M. Alvarez 2000. Use of Molecular Tools for Discriminating between two Populations of the Citrus Leafminer Parasitoid Ageniaspis (Hymenoptera: Encyrtidae). Ph.D. dissertation, Dept. of Entomology and Nematology, Univ. Florida, Gainesville. Google Scholar


J. M. Alvarez and M. A. Hoy . 2002. Evaluation of the ribosomal ITS2 DNA sequences in separating closely related populations of the parasitoid Ageniaspis (Hymenoptera: Encyrtidae). Ann. Entomol. Soc. Am 95:250–256. Google Scholar


C. P. Clausen 1931. Two Citrus Leaf Miners of the Far East. Tech. Bull. 252, U.S. Dept. Agric., Washington, DC. Google Scholar


J. B. Heppner 1993. Citrus leafminer, Phyllocnistis citrella, in Florida (Lepidoptera: Gracillariidae: Phyllocnistinae). Trop. Lepidoptera 4:49–64. Google Scholar


M. A. Hoy and R. Nguyen . 1994. Classical biological control of the citrus leafminer in Florida. Citrus Industry 75:661–62. Google Scholar


M. A. Hoy and R. Nguyen . 1997. Classical biological control of the citrus leafminer Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae): Theory, practice, art, and science. Trop. Lepidoptera 8:Suppl. 11–19. Google Scholar


M. A. Hoy, A. Jeyaprakash, R. Morakote, P. K C. Lo, and R. Nguyen . 2000. Genomic analyses of two populations of Ageniaspis citricola (Hymenoptera: Encyrtidae) suggest that a cryptic species may exist. Biol. Control 17:1–10. Google Scholar


K. C. Lo and S. C. Chiu . 1988. The Illustrations of Citrus Insect Pests and their Natural Enemies in Taiwan. Taiwan Agric. Res. Inst., Taichung, Taiwan. Google Scholar


C. Neale, D. Smith, G. A C. Beattie, and M. Miles . 1995. Importation, host specificity testing, rearing and release of three parasitoids of Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in eastern Australia. J. Aust. Entomol. Soc 34:343–348. Google Scholar


T. Ujiye, K. Kamijo, and R. Morakote . 1996. Species composition of parasitoids and rate of parasitism of the citrus leafminer (CLM), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in central and northern Thailand, with key to parasitoids of CLM collected from Japan, Taiwan and Thailand. Bull. Fruit Tree Res. Stn 29:79–106. [in Japanese with English abstract]. Google Scholar


J. A. Yoder and M. A. Hoy . 1998. Differences in water relations among the citrus leafminer and two different populations of its parasitoid inhabiting the same apparent microhabitat. Entomol. Exp. Applic 89:169–173. Google Scholar


Table 1. Survey of Jamaican citrus-growing areas for incidence of parasitism of the citrus leafminer by Ageniaspis citricola during 2005.

Published: 1 March 2007
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