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1 March 2013 Evidence of Establishment of Bagous hydrillae (Coleoptera: Curculionidae), a Biological Control Agent of Hydrilla verticillata (Hydrocharitales: Hydrocharitaceae) in North America?
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Abstract

The semi-aquatic weevil Bagous hydrillae was released during 1991–1996 at 19 sites in 4 states in attempts to control the aquatic weed hydrilla, Hydrilla verticillata. Fourteen of the sites were in Florida, 2 each in Texas and Georgia and one site in Alabama. Over 320,000 adult weevils were included in these releases. Despite the fact that a few adults were recovered as late as 4.5 yr post-release, presence of permanent, self-perpetuating populations was never confirmed. Then, during 2009 adult B. hydrillae were collected in southern Louisiana, at least 580 km from the nearest release site and 13 yr after attempts to establish this insect had terminated. This suggests that earlier recoveries were indicative of successful establishment and that this weevil species has persisted and dispersed widely in the southeastern USA. Nonetheless, there is no evidence that B. hydrillae has had a suppressive effect on hydrilla.

Hydrilla, Hydrilla verticillata (L.f.) Royle (Hydrocharitales: Hydrocharitaceae), a submersed, leafy-stemmed vascular hydrophyte, roots in the soil of water bodies and grows upwards producing thick floating mats at the water's surface. It is widely distributed in the Old World, but was introduced into the United States through the aquarium trade during the early 1950s (Schmitz et al. 1991). Infestations of hydrilla constitute the most severe aquatic plant problem in the southern United States. Hydrilla infested 22,000 ha in Florida by 1988 when the state was spending about $7 million annually in attempts to control about 6000 ha (Schmitz et al. 1991). During this same period it rapidly expanded its range and now occurs coast to coast (Sonder 1979) and as far north as New England (Les et al. 1997; Les & Mehrhoff 1999) and Washington state (Madeira et al. 2000). Southern populations tend to represent the dioecious biotype originally introduced into Florida; northern populations tend to be a monoecious biotype from a separate introduction in the Potomac River near Washington, D.C. (Steward et al. 1984; Madeira et al. 2000).

Currently, control of hydrilla is achieved primarily with herbicides or with exotic herbivorous fish (Sutton & Vandiver 1986; Langeland 1996), but both methods have limitations. Recently, hydrilla has become resistant to the most widely used and selective herbicide, fluridone (Michel et al. 2004), so alternatives are needed. One possible alternative involves the introduction of host-specific plant-feeding insects from the native range of hydrilla. Faunal studies aimed at finding potential biological control agents of hydrilla began during the 1970s in India and Pakistan and resumed during the 1980s in Africa, Asia, and Australia (Sankaran & Rao 1972; Baloch & Sana-Ullah 1974; Baloch et al. 1980; Pemberton 1980; Center et al. 1990; Zhang et al. 2010). These studies revealed the presence of several biological control candidates, 4 of which were ultimately released. One was the semi-aquatic weevil, Bagous hydrillae O'Brien (O'Brien & Askevold 1992).

Bagous hydrillae larvae mine hydrilla stems. It is native to Australia where it is easily extracted from hydrilla fragments found stranded on shorelines. Adults are not good swimmers but readily clamber about on submerged hydrilla feeding on the leaves creating distinctive “pepper shot” holes (Balciunas & Purcell 1991). Their feeding often notches the stems, thereby weakening them and causing them to fragment. Balciunas et al. (2002) reported that in Australia this putative “mowing” effect sometimes removed nearly all of the “topped-out” hydrilla in the upper portion of the water column. The female weevil inserts eggs singly into stem punctures usually near a leaf node (Balciunas & Purcell 1991). Larvae burrow within the stems further weakening the upper portions of the plant. Wind and wave action cause the hydrilla beds to break up stranding the fragments in windrows on exposed shorelines (Balciunas & Purcell 1991). After becoming fully grown, the third instar emerges from the stem to search for a pupation site. The larvae reportedly pupate within the stranded plant material or in the underlying soil. They probably also pupate in dewatered hydrosoil during the dry season after water levels recede or when temporary water bodies dry out. Adults fly at dusk and live about 5 wk (Balciunas & Purcell 1991).

Laboratory and field studies done in Australia (Balciunas et al. 1996) and in a Gainesville, Florida, quarantine facility (Buckingham 1994) verified the narrow host range of B. hydrillae. Accordingly, Buckingham petitioned the USDA Animal and Plant Health Inspection Service - Plant Protection and Quarantine (USDA-APHIS-PPQ) during Oct 1989 seeking permission to release this species (unpublished report). Permission was obtained after review by the Technical Advisory Group on Biological Control of Weeds (Coulson 1992), and this insect was released at hydrilla-infested field sites from 1991 to 1996 (Center & Grodowitz, unpublished data). However, it was widely believed that self-perpetuating populations had not established (Balciunas et al. 2002). Herein, we document these early releases, tentative evidence of establishment during that period, and a recent recovery of this species in southern Louisiana.

METHODS

Releases

Bagous hydrillae was first released on 8 Mar 1991 at Lake Osborne in Palm Beach Co., Florida. Additional releases were made at sites in Florida, Georgia, and Texas (Fig. 1, Table 1) until 1996. Over 320,000 adult weevils were released at 19 sites, all but 3 of which were in Florida. Adults were generally released directly onto exposed hydrilla but, in some cases, they were placed on fresh hydrilla several days prior to release, and then transported to sites on these sprigs. This infested material likely contained eggs and possibly young larvae. This plant material along with the adult weevils was placed amongst the resident hydrilla beds, sometimes inside cages to restrict dispersal. Evaluations were generally conducted by extracting the adults and larvae from samples of hydrilla using Berlese funnels. Voucher specimens were retained and identifications verified by a taxonomist (C. W. O'Brien). Later determinations were made by a project scientist (GW) after being trained by Dr. O'Brien. Details are provided in Grodowitz et al. (1994).

Louisiana Recoveries

Insects were sampled in mats of Salvinia minima Baker (Salviniaceae) in southern Louisiana as part of a different study during the summer of 2009 using floating pitfall traps as described by Parys & Johnson (2011). These traps consisted of a cylindrical glass container mounted into foam, and partially filled with preservative (ethylene glycol). A lid was mounted on plastic struts above the mouth of the cylinder to protect it from rain. The trap floated within the S. minima mat so that the mouth of the container was positioned at the same level as the surrounding plant material, and crawling invertebrates were passively trapped as they fell into the preservative. Each trap was tagged with a unique number denoting the location within the site. Insects collected in the traps were identified and sent to specialists for confirmation or further identification. C. W. O'Brien, who originally described and named B. hydrillae, identified the aquatic weevils. Bagous hydrillae specimens recovered from Louisiana were identified by C. W. O'Brien and independently by R. S. Anderson. These are deposited at the Louisiana State Arthropod Museum (LSAM) collection at Louisiana State University and in the private collection of Dr. O'Brien (CWOB).

Fig. 1.

Map of the southeastern United States showing the original sites where Bagous hydrillae was released (open squares), the sites where recoveries were made (solid dots), and the recent recovery in Louisiana (star). See Table 1 for detailed location information.

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RESULTS

Early Releases and Recoveries

During the period of active work on this project (1991–1996), 110 releases were made at 19 sites in 4 states (Table 1). The first evidence that the weevils might have established came from Lake Osborne in Palm Beach Co., Florida. More than 1000 adult weevils along with larval-infested plant material were released there from 26 Mar to 29 Jun 1991 and recoveries were made as late as 6 Nov 1991, 131 days after the final release. However, later efforts to find the weevils at the site were fruitless so it was assumed that the population failed to persist.

Weevils were recovered at several other sites within 2–3 months after the final releases, but these likewise failed to persist. However, a major effort to establish the beetles at Lake Seminole near the Florida-George border in Decatur Co., Georgia, seemed successful. Nearly 44,000 weevils were released between 23 Jun and 5 Sep 1992 near River Junction Landing and recoveries were made as late as 22 Oct 1993, nearly a full yr later.

A surprising find occurred at 2 sites, one in Texas and one in Florida. Eight releases totaling nearly 41,000 weevils were made at Choke Canyon, Live Oak Co., Texas, from May to Oct 1994 and B. hydrillae adults were recovered as late as 13 Dec 1994, 46 days after the final release. Seven releases totaling over 35,000 weevils were made in Bulldozer Canal, Brevard Co., Florida, during 29 Jan to 29 Oct 1993 with hundreds of weevils recovered 60 days later. Strangely, some of the weevils collected by Berlese extraction from Choke Canyon and all from Bulldozer Canal were identified by C. W. O'Brien as B. restrictus LeConte, a native species not known to utilize hydrilla and previously unrecorded from Florida (Center 1995).

The longest duration between release and recovery of B. hydrillae occurred at Wysong Dam on the Withlacoochee River in Sumter Co., Florida. Weevils were never actually released there, but they were released nearby at a site on Lake Panosofkee, less than 10 km away. They were never recovered at the release site but 2 B. hydrillae adults were found in a sample of hydrilla collected near the dam on 13 Aug 1996, more than 4.5 yr later. The presence of only 2 weevils after such a long time caused us to doubt this record and to suspect that the sample had become contaminated by escapees from a laboratory colony prior to Berlese extraction. This suspicion was reinforced when we recovered a single B. hydrillae in a Berlese sample of hydrilla from the Miami Canal in Broward Co., Florida, over 30 km from the nearest release site during Oct 1994 (Table 1). These records were therefore discounted and the lack of subsequent recoveries followed by the untimely termination of the project caused us to conclude that populations had not, in fact, permanently established at any location.

TABLE 1.

DATA ON BAGOUS HYDRILLAE RELEASES AND RECOVERIES MADE DURING 1991–1996.

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Louisiana Recoveries

This conclusion changed when one of us (KP) found bagoine weevils in pitfall samples from S. minima mats in southern Louisiana (Gramercy, Ascension Parish 31 Jul–16 Aug 2009, N 30° 09.804′W - 90 48.643>). Dr. O'Brien identified 2 of the specimens as B. hydrillae. This collection record was nearly 13 yr after the last known release in Florida and 580 km WSW of the nearest release at Lake Seminole, Georgia, and almost 750 km ENE of the next closest release at Choke Canyon, Texas (Fig. 1). The Gramercy site and nearby areas were revisited during Jun 2010 (by KP, MG, and SJ) but no hydrilla infestations were identified. This was almost a year after the pitfall samples had been collected so conditions could have changed. However, the site was a cypress-tupelo swamp with extensive coverage of floating macrophytes so it did not appear to be suitable habitat for hydrilla. Furthermore, the site had not visibly changed to any great extent during the interim.

DISCUSSION

Early failure to detect persistence of a released weed biological control agent followed by later evidence of population establishment is not unknown. Mo et al. (2000) reported that the rubber vine moth Euclasta whalleyi Popescu-Gorj & Constantinescu (Crambidae) had remained undetected for more than 4 yr in Australia after releases ceased, yet it later achieved outbreak densities on its host Cryptostegia grandiflora R. Br. (Gentianales: Apocynaceae). The chrysomelid beetle Zygogramma bicolorata Pallister disappeared after being introduced in Australia to control Parthenium hysterophorus L. (Asterales: Asteraceae) during 1980 but reappeared 10 yr later during periodic outbreaks (Dhileepan et al. 1996). The discovery of populations of biological control agents considerable distances from their release sites is also not unique to this example, as was recently documented (Pratt & Center 2012). Likewise, it isn't unusual for an agent to not be detected at a release site and then be found some distance away, as was the apparent case with the release of B. hydrillae at Lake Panosofkee and the recovery at Wysong Dam. We also had this experience when releasing the pyralid moth Niphograpta albiguttalis (Warren) on water hyacinth during the late 1970s (Center & Durden 1981) though in both cases recoveries were usually somewhat nearer release points.

The presence of B. hydrillae at a location that lacked hydrilla is puzzling. It is possible that hydrilla was present when samples were collected but had disappeared by the time weevil species had been determined and the site was re-inspected. Assuming that hydrilla was not present when the samples were collected, and with the absolute certainty regarding the identification of the beetle, 2 possibilities remain. One is that hydrilla existed somewhere elsewhere within the general area but not found when the site was revisited. The other possibility is that B. hydrillae was utilizing a plant other than H. verticillata as a host. The submersed aquatic plants bladderwort (Utricularia sp.; Lamiales: Lentibulariaceae), coon's tail (Ceratophyllumdemersum L; Ceratophyllales: Ceratophyllaceae), and naiad (Najas sp.; Hydrocharitaceae) were observed at and around the collection site. Buckingham & Balciunas (1994) noted that Najas guadalupensis (Sprengel) Magnus and N. tenuifolia R. Br. did produce a few adults during laboratory host range tests in Florida and Australia, respectively. So naiad, another serious aquatic weed, may have been supporting a marginal population of B. hydrillae at the site. This possibility merits further investigation.

The unexpected occurrence of B. restrictus on hydrilla at very distant B. hydrillae release sites remains a mystery. Only adults were found in both cases so there was no field evidence of its use of hydrilla as a developmental host, even though Wheeler & Center (2007) showed that it could develop in hydrilla stems. But dozens were extracted from the Bulldozer Canal, Florida samples, so it was not an incidental occurrence. The host plant of this native species is not known and no other potential host plants were noted at either location so further study is warranted.

The recent discovery of B. hydrillae in Louisiana suggests that earlier recoveries, such as that at Wysong Dam, represented small but persistent populations of the weevil. The most likely source for the Louisiana specimens was Lake Seminole on the Florida-Georgia border. Weevils were recovered at this site nearly a year after the releases were made which allowed time for them to disperse into other areas. Furthermore, this is the location nearest to the Louisiana site. Nonetheless, it now seems reasonable to conclude that B. hydrillae populations have established at a low level, although evidence of a corresponding reduction in hydrilla mats is lacking.

ACKNOWLEDGMENTS

A. Bishop, L. Bishop, W. C. Durden, L. Kasarjian, and B. Maharajh, USDA-ARS, Invasive Plant Research Laboratory, assisted in the rearing, release, and followup field work during the early years. Thanks to J. Fryoux and A. Meszaros for field assistance in Louisiana. We also thank R. S. Anderson, Canadian Museum of Nature, Ottawa, Ontario, Canada for confirmation of weevil identifications, J. Boyce for access to his property, and LSU AgCenter and Louisiana State Department of Wildlife and Fisheries for funding. The Florida Department of Environmental Protection, US Army Corps of Engineers, Southwest Florida Water Management District, and USDAARS provided funding for the initial project and their support is greatly appreciated.

REFERENCES CITED

  1. J. K. Balciunas , and M. F. Purcell 1991. Distribution and biology of a new Bagous weevil (Coleoptera, Curculionidae) which feeds on the aquatic weed, Hydrilla verticillata. J. Australian Entomol. Soc. 30: 333–338. Google Scholar

  2. J. K. Balciunas , D. W. Burrows , and M. F. Purcell 1996. Comparison of the physiological and realized host-ranges of a biological control agent from Australia for the control of the aquatic weed, Hydrilla verticillata. Biol. Control 7: 148–158. Google Scholar

  3. J. K. Balciunas , M. J. Grodowitz , A. F. Cofrancesco , and J. F. Shearer 2002. Hydrilla, pp. 91–114 In R. VanDriesche , B. Blossey , M. Hoddle and R. Reardon [eds.], Biological Control of Invasive Plants in the Eastern United States. USDA Forest Service Publication FHTET-2002-04, Morgantown, WV. Google Scholar

  4. G. M. Baloch , and Sana-Ullah . 1974. Insects and other organisms associated with Hydrilla verticillata (L.f.) L.C. (Hydrocharitacea) in Pakistan, pp. 61–66 In A. J. Wapshere [ed.], Proc. III Intl. Symp. on Biological Control of Weeds, 10–14 Sep 1973, Montpellier, France, Misc . Publ. No. 8, Commonwealth Institute of Biological Control. . Google Scholar

  5. G. M. Baloch , Sana-Ullah , and M. A. Ghani 1980. Some promising insects for the biological control of Hydrilla verticillata in Pakistan. Trop. Pest Mgt. 26: 194–200. Google Scholar

  6. G. R. Buckingham 1994. Biological control of aquatic weeds, pp. 413–480 In D. Rosen , F. D. Bennett , and J. L. Capinera [eds.], Pest Management in the Subtropics. Biological Control - a Florida Perspective. Intercept Ltd, Andover, Hampshire, UK, Google Scholar

  7. G. R. Buckingham , and J. K. Balciunas 1994. Biological studies of Bagous hydrillae. Tech. Rep. A-94-6, U.S. Army Corps of Engineers, Waterways Experiment Station, Vicksburg, MS. Google Scholar

  8. T. D. Center 1995. The application of foundational research in biological control projects, pp. 106–114 In Proc. 29th Annu. Mtg., Aquatic Plant Control Res. Program, U.S. Army Corps of Engineers, Waterways Experiment Station. Misc. Paper A-95-3. Google Scholar

  9. T. D. Center , and W. C. Durden 1981. Release and establishment of Sameodes albiguttalis for the biological control of waterhyacinth. Environ. Entomol. 10: 75–80. Google Scholar

  10. T. D. Center , A. F. Cofrancesco , and J. K. Balciunas 1990. Biological control of aquatic and wetland weeds in the southeastern United States, pp. 239–262 In E. S. Delfosse [ed.], Proc. VII Intl. Symp. on Biological Control of Weeds, 6–11 Mar 1988, Rome, Italy, Istituto Sperimentale per la Patologia Vegetale (Ministero dell'Agricoltura e delle Foreste), Rome, Italy. Google Scholar

  11. J. R. Coulson 1992. The TAG: Development, functions, procedures, and problems, pp. 53–60 In R. Charudattan and H. W. Browning [eds.], Regulations and Guidelines: Critical Issues in Biological Control. Proc. USDA/CSRS Natl. Wkshp., 10–12Jun 1991, Vienna, VA. IFAS, Univ. Florida, Gainesville. Google Scholar

  12. K. Dhileepan , B. Madigan , M. Vitelli , R. McFadyen , K. Webster , and M. Trevino 1996. A new initiative in the biological control of Parthenium , pp. 309–312 In R. C. H. Shepherd [ed.], Proc. 11th Australian Weeds Conf., Weed Sei. Soc. Victoria, Melbourne, Australia. Google Scholar

  13. M. J. Grodowitz , T. D. Center , E. Snoddy , and F. A. Dray 1994. Release and establishment of insect biocontrol agents for the management of hydrilla, pp. 181–201 Proc. 28th Annu. Mtg., Aquatic Plant Control Res. Program, U.S. Army Corps of Engineers, Waterways Exp. Sta. Misc. Paper A-94-2. Google Scholar

  14. K. A. Langeland 1996. Hydrilla verticillata (L.F.) Royle (Hydrocharitaceae), The Perfect Aquatic Weed. Castanea 61: 293–305. Google Scholar

  15. D. H. Les , and L. J. Mehrhoff 1999. Introduction of nonindigenous aquatic vascular plants in southern New England: a historical perspective. Biol. Invasions 1: 281–300. Google Scholar

  16. D. H. Les , L. J. Mehrhoff , M. A. Cleland , and J. D. Gabel 1997. Hydrilla verticillata (Hydrocharitaceae) in Connecticut. J. Aquat. Plant Mgt. 35: 10–14. Google Scholar

  17. P. T. Madeira , C. C. Jacono , and T. K. Van 2000. Monitoring hydrilla using two RAPD procedures and the nonindigenous aquatic plant species database. J. Aquat. Plant Mgt. 38: 33–40. Google Scholar

  18. A. Michel , R. S. Arias , B. E. Scheffler , S. O. Duke , M. Netherland , and F. E. Dayan 2004. Somatic mutation-mediated evolution of herbicide resistance in the nonindigenous invasive plant hydrilla (Hydrilla verticillata). Mol. Ecol. 13: 3229–3237. Google Scholar

  19. J. Mo , M. Trevino , and W. A. Palmer 2000. Establishment and distribution of the rubber vine moth, Euclasta whalleyi Popescu-Gorj and Constantinescu (Lepidoptera: Pyralidae), following its release in Australia. Australian J. Entomol. 39: 344–350. Google Scholar

  20. C.W. O'Brien , and I. S. Askevold 1992. Systematics and evolution of weevils of the genus Bagous Germar (Coleoptera, Curculionidae). 1. Species of Australia Trans. American Entomol. Soc. 118: 331–452. Google Scholar

  21. K. A. Parys , and S. J. Johnson 2011. Collecting insects associated with wetland vegetation: an improvied design for a floating pitfall trap. Coleopts. Bull. 65: 341–344. Google Scholar

  22. R. W. Pemberton 1980. Exploration for natural enemies of Hydrilla verticillata in Eastern Africa. U.S. Army Eng., Waterways Exp. Sta., Misc. Paper A-80-1: 52 pp. Google Scholar

  23. P. D. Pratt , and T. D. Center 2012. Biocontrol without borders: the unintended spread of introduced weed biological control agents. BioControl 57: 319–329 Google Scholar

  24. T. Sankaran , and V. P. Rao 1972. An annotated list of insects attacking some terrestrial and aquatic weeds in India, with records of some parasites of the phytophagous insects. Commonwealth Inst. Biol. Control Tech. Bull. 15: 131–157. Google Scholar

  25. D. C. Schmitz , B. V. Nelson , L. E. Nall , and J. D. Schardt 1991. Exotic aquatic plants in Florida: a historical perspective in review of the present aquatic plant regulation program, pp. 303–323 In T. D. Center , R. F. Doren , R. L. Hofstetter , R. L. Myers and L. D. Whiteaker [eds.], Proc. Symp. on Exotic Pest Plants, Miami, FL. 2-4 Nov. 1988. U.S. Dept. Interior, Tech. Rep. NPS/NREVER/NRTR-91/06, Nat. Park Serv., Washington, D.C. Google Scholar

  26. L. W. Sonder 1979. Hydrilla infestations in California, pp. 122–125 In Proc. California Weed Conf., Sacramento, CA. Google Scholar

  27. D. L. Sutton , and V. V. Vandiver Jr 1986. Grass carp: a fish for biological management of hydrilla and other aquatic weeds in Florida. Univ. Florida, Agr. Exp. Sta., Gainesville, FL. Google Scholar

  28. K. K. Steward , T. K. Van , V. Carter , and A. H. Pieterse 1984. Hydrilla invades Washington, D.C. and the Potomac. American J. Bot. 71: 162–163. Google Scholar

  29. G. S. Wheeler , and T. D. Center 2007. Hydrilla stems and tubers as hosts for three Bagous species: Two introduced biological control agents (Bagous hydrillae and B. affinis) and one native species (B. restrictus). Environ. Entomol. 36: 409–415. Google Scholar

  30. J. Zhang , G. S. Wheeler , M. Purcell , and J. Ding 2010. Biology, distribution, and field host plants of Macroplea japana in China: An unsuitable candidate for biological control of Hydrilla verticillata. Florida Entomol. 93: 116–119. Google Scholar

Ted D. Center, Katherine Parys, Mike Grodowitz, Gregory S. Wheeler, F. Allen Dray, Charles W. O'Brien, Seth Johnson, and Al Cofrancesco "Evidence of Establishment of Bagous hydrillae (Coleoptera: Curculionidae), a Biological Control Agent of Hydrilla verticillata (Hydrocharitales: Hydrocharitaceae) in North America?," Florida Entomologist 96(1), 180-186, (1 March 2013). https://doi.org/10.1653/024.096.0124
Published: 1 March 2013
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