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1 March 2014 First Larval Description of the Coastal Genus and Species Phucobius simulator (Coleoptera: Staphylinidae)
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Abstract

The late-instar larva of Phucobius simulator Sharp is described for the first time, being also the first larval description of the genus Phucobius Sharp. Nine unknown larvae were collected in association with adults of P. simulator from seashores of Korea and Japan. The unknown larvae were identified as P. simulator by DNA sequencing. Diagnostic characters of the species are provided, with illustrations.

The genus Phucobius Sharp contains 8 species (Herman 2001) and is confined to the seashores of the Oriental and eastern Palearctic Regions, and East Africa. Adults of the genus Phucobius are similar to those of the genus Cafius Stephens but lack spines on the anterior tibiae (Moore & Legner 1976; Smetana 1995). Adults and larvae are found under accumulated decaying seaweeds and logs. To date no late-instar larvae of the genus Phucobius have ever been described.

In this paper, we describe late-instar larvae of P. simulator for the first time through the association of larvae-adults with DNA sequences, and provide diagnostic characters with illustrations.

Late-Instar Larva of Phucobius Simulator Sharp (Fig. 1)

  • Diagnostic Combination

    Among coastal staphylinid genera, late-instar larvae of Phucobius simulator are recognized by the following combination of characters: neck present; antenna with article 2 longer than 3, article 3 with 2 solenidia and 1 campaniform sensillum (Fig. 2); stipes a little shorter than maxillary palpus, mala with 1 apical seta (Fig. 3); mandible slender, with tiny internal tooth (Fig. 4); nasale middle 3 teeth distinctly separated from lateral teeth (Fig. 5); ligula short, 0.32 times as long as labial palpomere 1 (Fig. 6); tarsungulus with 3 spines (Fig. 7); tergite X shorter than 1st article of urogomphi.

  • Description

    Length 11.0–13.0 mm. General body shape elongate, flattened, parallel-sided (Fig. 1). Body brown, but head light brown, abdomen dark brown.

  • Head

    Sub-quadrate, almost equally wide from apical to basal margin. About 0.8 times as long as wide. Four stemmata present. Ecdysial sutures distinct and complete from near antennal insertion. Antenna (Fig. 2), 4-articled. Length of articles 1st = 4th < 3rd < 2nd; article 1 wider than long, transverse; 1 campaniform sensillum present on 1/3 of article 2; article 3 with 2 solenidia (IIIS1 and IIIS2) and 1 campaniform sensillium, 1 corn-type sensory appendage present; article 4 with 4 solenidia (IVS1-IVS4); article 3 and 4 each with 3 setae. Mentum with 3 pairs of setae. Guiar sutures convergent in middle of head, divergent to apex.

  • Mouthparts

    Maxilla (Fig. 3). Stipes a little shorter than maxillary palpus; mala with 1 seta at apex, small, elongate; maxillary palpus with 4 articles, a separate sclerotization forming a short ring at base in form of an extra article present, 1 seta and 1 campaniform sensillum present; length ratio of palpomeres 1st : 2nd : 3rd : 4th = 1:1.43:1.14:0.36; width of palpomeres 1st = 2nd > 3rd > 4th. Mandible (Fig. 4) slender, 2 macrosetae present along outer surface, falciform, undivided at acute apex, tiny internal tooth present on apical region, almost symmetrical. Nasale (Fig. 5). Anterior margin of nasale with 9 teeth divided into 3 distinct clusters (1 middle and 2 lateral), each cluster with 3 teeth; middle 3 teeth pointed (LT4 and LT5), central tooth (LT5) smallest, the last and penultimate teeth (LT 1 and LT2) very weak. Ten setae present on each side of midline. Labium (Fig. 6). Labial palpus with 3 articles; length ratio of palpomeres 1st : 2nd : 3rd = 1.0:0.47:0.16, conical ligula short, 0.32 times as long as palpomere 1, pubescent at base.

  • Thorax

    Pronotum subquadrate, sclerotized, setae scattered at sides and on disc. Pronotum about 2 times longer than mesonotum. Mesonotum length subequal to metanotum, both as long as posterior margin of pronotum.

  • Legs (Fig. 7)

    Coxa, trochanter, femur, tibia, and tarsungulus distinguishable, tarsungulus with 3 articulated spines.

  • Abdomen

    Abdominal tergites I—VIII transverse, parallel-sided, slightly narrowed to apex. Tergites and sternites divided by mid-longitudinal line; tergite X about 5.0 times longer than wide. Urogomphi two-articled, longer than tergite X; article 1 slender, longer than tergite X; article 2 with 2 small setae and 1 large seta arising from apex.

  • Specimens Examined

    KOREA: Gyeongnam Prov., Geoje Isl., Hwangpo beach, 28-VI-2004, J.-S. Park and K.-J. Ahn, ex under seaweeds (CNUIC, 1); Gangwon Prov., Gangneung-si, Geumjin-ri, Okgye beach, N 37° 37′ 37.2″ E 129° 03′ 04.8″ 7 m, 4-IX-2013, K.-J. Ahn, I. S. Yoo, J. H. Song, under log near estuary (CNUIC, 2); Japan: Hokkaido, Nemuro, Shunkunitai, 24-VIII-1999, K.-J. Ahn, ex under stones on salt marsh (CNUIC, 6).

  • Remarks

    Most specimens were collected under logs or stones in an estuary (Fig. 8). The late-instar larva of Phucobius simulator resembles that of Cafius vestitus (Sharp) in shape and structures of mouthparts including the teeth on the nasale. They are also similar in the shape of antenna and urogomphi. But they differ from each other in the ratios of following characters presented in Table 1.

  • Fig. 1.

    Phucobius simulator. Habitus of larva, length 12.0 mm.

    f01_203.jpg

    Figs. 2-7.

    Phucobius simulator. 2, Antenna, dorsal aspect; 3, maxilla, dorsal aspect; 4, mandible, dorsal aspect; 5, nasale, dorsal aspect; 6, labium, ventral aspect; 7, anterior leg, dorsal aspect. Scale bars = 0.1 mm.

    f02_203.jpg

    Fig. 8.

    Collection site of Phucobius simulator larvae in an estuary at Okgye Beach, Korea. Inset is a beetle larva collected in the microhabitat (under log or stone) indicated by an arrow.

    f08_203.jpg

    Additional Molecular Evidence For The Identification of Late-Instar Larvae of P. Simulator

    Unknown larvae were collected with adults of Phucobius simulator from seashores of Korea and Japan, and we attempted to associate them by gene sequencing of individuals. The partial cytochrome oxidase II gene was sequenced from the unknown larvae and several identified adult specimens of Phucobius (see Jeon & Ahn 2007 for the method). The sequences are deposited in GenBank under accession numbers (Table 2).

    Table 1.

    Differences between late-instar larvae of Phucobius simulator and Cafius vestitus.

    t01_203.gif

    Table 2.

    Species, collection information, and Genbank accession numbers for Cytochrome oxidase II sequences investigated in this study to identify larvae collected in association with adults of Phucobius simulator from seashores of Korea and Japan.

    t02_203.gif

    In previous studies of the genus Cafius known to be closely related to Phucobius (Jeon & Ahn 2005, 2007; Jeon et al. 2012), the intraspecific pdistance of cytochrome oxidase II gene varied in the range 0-2.93% and the minimum interspecific p-distance was 11.79%. The pairwise distances of P. simulator between adult and larva were 1.23-1.72%, placed within the range of intraspecific p-distances compared to the former results.

    We added the present Phucobius data (Table 2) to the previous Cafius data (Jeon & Ahn 2007) and performed a parsimony analysis with CO II genes in TNT 1.1 (Goloboff et al. 2007) using the implicit enumeration option. The length of the COII sequences included in the analysis was 344 bp. The branch support values were estimated by bootstrapping with 100 replications. The analysis showed that specimens of a single species formed cohesive assemblages (Fig. 9). The unknown larva grouped unambiguously with the adult specimens of Phucobius simulator. Therefore, we identified the unknown larvae as probable late-instar larvae of P. simulator.

    Fig. 9.

    Bootstrapping results in TNT based on 100 replications with branch support values.

    A parsimony analysis in TNT 1.1 was performed on the CO II Phucobius and Cafius 344 bp data, and the branch support values were estimated by bootstrapping with 100 replications.

    f09_203.jpg

    Acknowledgments

    We thank the Willi Hennig Society for sponsorship of the program, TNT. Comments by J. H. Frank (Univ. of Florida, Gainesville) significantly improved the manuscript. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0073111 and 2012-031412).

    REFERENCES CITED

    1. P. Goloboff , J. Farris , and K. Nixon 2007. T.N.T. version 1.1: Tree analysis using new technology. Program and documentation, available from the authors, and at  www.zmuc.dk/public/phylogenyGoogle Scholar

    2. L. H. Herman 2001. Catalog of the Staphylinidae (Insecta: Coleoptera). 1758 to the end of 161 the second millennium. Parts I-VII. Bull. Amer. Mus. Nat. Hist. 265: 1–4218. Google Scholar

    3. M.-J. Jeon , and K.-J. Ahn 2005. First larval descriptions for Cafius Curtis (Coleoptera: Staphylinidae: Staphylininae) in Korea. J. Kansas Entomol. Soc. 78(3): 261–271. Google Scholar

    4. M.-J. Jeon , and K.-J. Ahn 2007. Descriptions of lateinstar larvae of three littoral Cafius species (Coleoptera: Staphylinidae) by association of life stage using DNA sequences. Florida Entomol. 90(3): 465–474. Google Scholar

    5. M.-J. Jeon , J.-H. Song , and K.-J. Ahn 2012. Molecular phylogeny of the marine littoral genus Cafius (Coleoptera: Staphylinidae: Staphylininae) and implications for classification. Zool. Scr. 41(2): 150–159. Google Scholar

    6. I. Moore , and E. F. Legner 1976. Intertidal rove beetles (Coleoptera: Staphylinidae). Chapter 19, pp. 521–551 In L. Cheng [ed.], Marine Insects. North Holland Publisher, New York. Google Scholar

    7. A. Smetana 1995. Rove beetles of the subtribe Philonthina of America north of Mexico (Coleoptera: Staphylinidae). Classification, phylogeny and taxonomie revision. Mem. Entomol., Intl. 3: 1–946. Google Scholar

    Mi-Jeong Jeon and Kee-Jeong Ahn "First Larval Description of the Coastal Genus and Species Phucobius simulator (Coleoptera: Staphylinidae)," Florida Entomologist 97(1), (1 March 2014). https://doi.org/10.1653/024.097.0126
    Published: 1 March 2014
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