The green lacewing, Mallada basalis (Walker) (Neuroptera: Chrysopidae), is one of the most important natural enemies used in biological control programs for forestry and agricultural pests. However, it is difficult to mass rear M. basalis instars because they are voracious cannibals. This study was conducted to determine the effect of cannibalism on the development and fecundity of M. basalis. We analyzed the developmental parameters of 3rd instars that cannibalized M. basalis eggs and 1st instars, and the relationship between the proportion of each of the 3rd instars cannibalized and the number of folded paper barriers provided in an arena to minimize the frequencies of encounters. In all treatments the green lacewing instars were provided eggs of the pyralid, Corcyra cephalonica (Stainton). In Treatment I the pyralid eggs were supplemented with M. basalis eggs and in Treatment II, the pyralid eggs were supplemented with M. basalis 1st instars. The developmental times of the 3rd instars and pupae of Treatment I and Treatment II were significantly shorter than in the control. The pupal weights, fecundities and egg hatch rates of Treatments I and II were significantly larger than in the control. When 3rd instars cannibalized M. basalis eggs or 1st instars, the pupal weights significantly increased from 10.3 (control) to 11.0 and 10.9 mg, respectively, fecundities significantly increased from 91 (control) to 141 and 121 eggs, respectively, and the egg hatch rates significantly increased from 62% (control) to 70% and 68%, respectively. Moreover, as the number of folded paper barriers per arena increased, the proportions of 3rd instars cannibalized at first tended to decrease but then increased. When the different instars were studied separately, the proportion of 3rd instars cannibalized was larger than the corresponding proportions of 1st and 2nd instars. The research indicated that cannibalism enhances both development and fecundity of M. basalis. The use of paper barriers to frequent encounters reduced cannibalism somewhat, but this method was not adequately effective for the 3rd instars. Our findings may contribute to the mass production of this economically important predatory green lacewing.
The green lacewing, Mallada basalis (Walker) (Neuroptera: Chrysopidae), distributed in South China, is one of the most important natural enemies used in the biological control of insect pests of forestry and agriculture (Ye et al. 2013). Its larvae prey on several kinds of pests, such as mealy bugs, whiteflies, the eggs and larvae of Lepidoptera, and other orders (Li et al. 2011). However, M. basalis displays substantial cannibalism which may impede mass rearing. Also, larval cannibalism in apredatory insect species is a manifestation of significant intra-specific competition.
Cannibalism, defined as killing and eating an individual of the same species, is a behavioral trait seen in a number of species. Cannibalism occurs in many different ecological and social contexts, and is present during any or all life stages from just after hatching and throughout development or mating (Fox 1975; Elgar & Crespi 1992). Cannibalism has been observed in a number of animals and is seen as a force that structures predatory insect communities (Polis et al. 1989; Dong & Polis 1992). Newly eclosed coccinellid larvae may improve their survival rate by feeding on conspecific eggs (Gagné et al. 2002), and larval cannibalism of coccinellids increases their rates of success of pupation and emergence (Wang 2010). Thus, cannibalism is adaptively significant for ladybirds to raise their survival rate in adversity (Agarwala & Dixon 1992). Female mantis (Mantodea: Mantidae) and spiders (Arachnida: Araneae) may gain nutritional benefits from male consumption that consequently translates into increased fecundity (Birkhead et al. 1988; Newman & Elgar 1991; Barry et al. 2008).
The biology and ecology of Chrysopa sinica Tiedet, Chrysopa carnea Stephens, Chrysopa formosa Brauer, Chrysopa phyllochrom Brauer have been studied in China and other countries. Techniques for improving the mass rearing of these green lacewings have been studied, including the use of folded paper, straws and copper wire as partitions to reduce cannibalism during the mass rearing (Cai et al. 1983; Xu et al. 1997; Su et al. 1999; Zhang et al. 2004; Muhammad et al. 2006; Li et al. 2010). However, there has been little research on the mass rearing of M. basalis, and especially on the biological significance and the mechanism of larval cannibalism. Thus, the effect of cannibalism, in which the 3rd instars cannibalize eggs and 1st instars, on the development and fecundity of M. basalis, and the relationship between the proportion of larvae cannibalized and different amounts of folded paper were studied, to provide a theoretical foundation and technical support for mass rearing.
Materials and Methods
Mallada basalis larvae were collected in 2010 from guava (Psidium guajava L.; Myrtales: Myrtaceae) trees in Wenchang City, Hainan Province, and reared in the laboratory with eggs of the rice moth [Corcyra cephalonica Stainton) (Pyralidae)] (Ye et al. 2012). The 13th generation of M. basalis was chosen for experiments. Corcyra cephalonica, whose eggs were irradiated with ultraviolet light for 30 min to kill their embryos before being used, was reared with rice bran. Rearing conditions were 26 ± 1 °C, 70 ± 5% RH and 16:8 h L:D.
Developmental Parameters of Mallada basalis, under Various Intra-Specific Feeding Conditions
About 100 newly hatched M. basalis larvae were individually reared until the 3rd instar with eggs of C. cephalonica and with moistened cotton enclosed in a little glass tube. Then, two treatments were carried out. In Treatment I each M. basalis 3rd instar was put in a Petri dish (9 cm diam × 1.5 cm) and reared with 50 C. cephalonica eggs (Li et al. 2012) and with cotton moistened in water every day. Five M. basalis eggs were added to feed each M. basalis 3rd instar every day until the 3rd instars pupated. Next, the pupa was weighed and its weight was recorded. After the adults emerged, their sex was recorded, and then each adult female was paired with a male and put into a Petri dish, and fed brewer's yeast powder, honey and water moistened in cotton. The female's fecundity (eggs laid per day) and longevity were recorded. After the adults had laid eggs, one hundred eggs were randomly chosen, and their hatching rate was recorded. Ten Petri dishes were used for each treatment, which was repeated 5 times.
In Treatment II five M. basalis 1st instars were added everyday to feed each M. basalis 3rd instar until the 3rd instars pupated; other steps were the same as Treatment I. In the control neither M. basalis eggs nor 1st instars were added to each Petri dish, and other steps were the same as Treatment I. The experiments were carried out in a growth chamber at 26 ± 1 °C, 70 ± 5% RH and 16:8 h L:D.
Relationship between the Proportion of Larvae Cannibalized and Number of Paper Barriers
Five M. basalis larvae were reared in a 9 cm diam × 1.5 cm Petri dish with 50 C. cephalonica eggs and moistened cotton. A paper strip (7 cm × 2.5 cm), folded twice along the long axis, was placed into the Petri dish as a barrier to frequent encounters of larvae. Then, no piece, or 1, 2, 3, and 4 pieces of similarly folded paper, respectively, were put into various Petri dishes. The following 3 treatments were carried out separately with newly hatched larvae, 2nd instars and 3rd instars. The number of larvae that were killed was recorded each day. Such records were taken until the next molt, i.e., in Treatment #1 until all of the 1st instars had transformed into 2nd instars, in Treatment #2 until all of the 2nd instars had transformed into 3rd instars, and in Treatment #3 until all of the 3rd instars had pupated. The proportion of cannibalized larvae was calculated by dividing the number killed by the initial number of M. basalis larvae (5).
Data were subjected to statistical analysis (SPSS Inc., Chicago, Illinois, USA). Differential effects of cannibalism on development parameters and the developmental times of third instars, pupae and adults, and the relationship between the proportion of cannibalized larvae and the number of paper barriers to encounters were analyzed by one-way analysis of variance (ANOVA). These analyses were performed on data of 5 replicates per treatment, and the means were separated by Tukey's test at P ≤ 0.05.
Developmental times (days) of Mallada basalis third instars, pupae and adults when 3rd instars cannibalized either M. basalis eggs or 1st instars.
Effect of Cannibalism on Developmental Times of Various Life Stages
The developmental times of adults (Table 1) did not differ significantly among the control, Treatment I (cannibalizing eggs) and Treatment II (cannibalizing 1st instars) (F = 0.254; df = 113; P = 0.776). However, there were significant differences in the developmental times of the 3rd instars and pupae between the control and Treatments I and II (F = 16.53, 10.75; df = 149, 143; P < 0.05). The developmental times of the 3rd instars and the pupae of the control were 5.06 days and 9.13 days respectively; those of Treatment I were significantly shorter at 4.32 days and 8.61 days, respectively, and those of Treatment II also were significantly shorter than the control, i.e., 4.14 days and 8.69 days, respectively (Table 1).
Effect of Cannibalism on Developmental Parameters
Different treatments had no significant influence on the sex ratio (Table 2) with the proportion of females ranging insignificantly from 0.51 to 0.60 (F = 3.24, df = 14, P = 0.075). The pupation rate and emergence rate did not differ significantly among the control, Treatment I and Treatment II (F = 0.46, 3.81; df = 14; P = 0.64, 0.06). However, there were significant differences in the pupal weights, fecundity and egg hatching rate among the control and other treatments (F = 41.02, 16.37 and 16.46, respectively; df = 143, 63 and 14, respectively; P < 0.05). In individuals that had cannibalized eggs or 1st instars, the pupal weight was significantly increased to 10.96 mg or 10.88 mg, as compared to 10.33 mg in the control. Fecundity in Treatment I was 140.50 eggs, and in Treatment II it was 120.77 eggs, and these levels were significantly larger than the control level of 90.56 eggs. Also the egg hatch rates were 69.60% in Treatment I and 67.80% in Treatment II, both of which were significantly larger than the control, 61.80% (Table 2).
Developmental parameters of Mallada basalis when 3rd instars cannibalized either M. basalis eggs or 1st instars.
Relationship between the Proportion of Larvae Cannibalized and Number of Paper Barriers
Generally the larvae did not hide in the folded paper barriers; however the 3rd instars tended to hide in the paper crevices just prior to pupation. Fig. 1, shows that as the number of folded paper barriers increased, the proportions of all 3 instars that were cannibalized tended to decrease at first and then to increase. There were significant differences among the proportions of cannibalized instars and the number of folded paper barriers (F = 3.07, 5.63 and 7.89, respectively; df = 24; P < 0.05). The proportion of cannibalized 3rd instars was the largest regardless of the number of barriers to encounters. The proportion of cannibalized 3rd instars was the largest with no paper barrier, i.e., 0.50, whereas with 2 paper barriers it was the smallest, i.e., 0.24. The proportion of cannibalized 2nd instars was smaller than that of 1st and 3rd instars. The proportion of cannibalized 2nd instars was the largest with no paper barrier, i.e., 0.14, and it was the smallest with 2 paper barriers, i.e., 0.05. The proportion of cannibalized 1st instars was the largest with no paper barrier, i.e., 0.33, while with 3 barriers it was the smallest, i.e., 0.10.
Studies on cannibalism in arthropods have revealed several important factors affecting the occurrence of cannibalism: size, developmental stage, hunger, density, and food availability. The first 3 factors largely determine the vulnerability of the victim, whereas the latter 2 factors largely affect the environmental potential for cannibalism (Dong & Polis 1992). Studies of the proximate factors of cannibalism have found that a relative size difference between the cannibal and its victim is the most frequently cited factor determining the vulnerability of the victim, with developmental stage and hunger level are considered to be secondary determinants (Semlitsch & West 1988; Dong & Polis 1992; Lounibos et al. 1996; Sowig 1997; Samu et al. 1999).
The larvae of green lacewings have the habit of killing each other (Ridgeway et al. 1970). Field experiments have established that predatory terrestrial arthropods are frequently food- and nutrient-limited in nature (Wise 1993; Fagan et al. 2002; Denno & Fagan 2003; Fagan & Denno 2004; Wise 2006), and these limitations may be the reason that cannibalism occurs. In this paper the eggs and 1st instars were used to feed the 3rd instars. Even though sufficient C. cephalonica eggs were provided as food, cannibalism still happened. Qiu (1997) found that in mass rearing, if food was insufficient, cannibalism intensified, and if food was sufficient, cannibalism still could not be completely avoided. In this paper, the developmental times of the 3rd instars and pupae were shorter when they cannibalized eggs and 1st instars than when no cannibalism occurred. and when 3rd instars were allowed to cannibalize eggs and 1st instars, their pupal weights were significantly larger than those of 3rd instars that had no opportunity to cannibalize. These results indicated that cannibalism favored the growth and development of larvae and pupae. Also cannibalism significantly increased fecundity and percent egg hatch. Thus our findings indicated that cannibalism was positive for the experimental population.
Cannibalism has effects at both the individual and the population level. At the individual level, a cannibal gains food, whereas from the point of view of the victim, cannibalism increases the death rate (Frank & Wilfried 1997). In the study on impeding encounters between and among M. basalis instars by folded paper barriers, we found that as the number of barriers increased, the proportion of cannibalized all three instars tended to decrease at first and then to increase, and there were significant differences among the number of folded paper barriers and the proportion of cannibalized instars. This suggested that use of paper barriers can indeed reduce cannibalism to a limited extent. The more barriers provided, the less the amount of space was available per larva, and thus as crowding increased, it became easier for the larvae to meet and cannibalize. Therefore, the increased use of paper barriers caused the rate of cannibalism to increase. We found that the cannibalism proportion of 3rd instars was the largest. It could be that the tendency toward cannibalism of 3rd instars was much greater than in the earlier instars. Thus it may be necessary to mass rear 3rd instars by keeping them individually in separate cells. Perhaps surprisingly the proportion of dead 1st instars was larger than of 2nd instars, which might be due to a lower survival rate of neonates. In an experiment using paper barriers, cannibalism was greater in the first instars of Chrysopa septempunctata Wesmael larvae and the survival rate was the lowest for the 1st instars, which indicated that more barriers to encounters were needed for rearing first instars (Zhang et al 2004). This was different from our results.
Our findings may contribute to the mass production of this economically important predatory green lacewing. However, several issues require further study, such as the correlation between larval density and cannibalism as well as between food availability and cannibalism.
This study was supported by the Youth Foundation of Guangdong Academy of Sciences (qnjjsq201113), the Special Fund for Agro-scientific Research in the Public Interest (201103026-1) and the Science and Technology Plan Project of Guangdong Province (2011B031500026).
- B. K. Agarwala , and A. F. G. Dixon 1992. Laboratory study of cannibalism and interspecific predation in ladybirds. Ecol. Entomol. 17 (4): 303–309. Google Scholar
- L. Barry K , G. I. Holwell , and M. E. Herberstein 2008. Female praying mantids use sexual cannibalism as a foraging strategy to increase fecundity. Behav Ecol. 19: 710–715. Google Scholar
- T. R. Birkhead , K E. Lee , and P. Young 1988. Sexual cannibalism in the praying mantis Hierodula membranacea. Behav. 106: 112–118. Google Scholar
- C. R. Cai , X. D. Zhang, and J. Z. Zhao 1983. Study on the liquid artificial diet of Chrysopa sinica larvae. Natural Enemies of Insects 5(2): 82–85. Google Scholar
- R. F. Denno , and W. F. Fagan 2003. Might nitrogen limitation promote omnivory among carnivorous arthropods? Ecol. 84: 2552–2531. Google Scholar
- Q. Dong , and G. A. Polis 1992. The dynamics of cannibalistic populations: A foraging perspective, pp. 13–37 In M. A. Elgar and B. J. Crespi [eds.], Cannibalism: Ecology and Evolution among Diverse Taxa. Oxford Science Publications. Oxford. Google Scholar
- M. A. Elgar , and B. J. Crespi 1992. Cannibalism: Ecology and Evolution among Diverse Taxa. Oxford University Press. Oxford. Google Scholar
- W. F. Fagan , and R. F. Denno 2004. Stoichiometry of actual vs. potential predator-prey interactions: insights into nitrogen limitation for arthropod predators. Ecol. Lett. 7: 876–883. Google Scholar
- W. F. Fagan , E. Seimann , C. Mitter , R. F. Denno , A. F. Huberty , H. A. Woods , and J. J. Elser 2002. Nitrogen in insects: implications for trophic complexity and species diversification. American Nat. 160: 784–802. Google Scholar
- V. D. B. Frank , and G. Wilfried 1997. Cannibalism in an age-structured predator-prey system. Bull. Math. Biol. 59(3): 551–567. Google Scholar
- L. R. Fox 1975. Cannibalism in natural populations. Annu. Rev. Ecol. Sys. 6: 87–106. Google Scholar
- I. Gagne , D Coderre , and Y. Mauffette 2002. Egg cannibalism by Coleomegilla maculata lengi neonates: Preference even in the presence of essential prey. Ecol. Entomol. 27: 285–291. Google Scholar
- G. P. Li , L. Ma , H. Q. Feng , and F. Qiu 2010. Rearing successive generations of Chrysolerla nippoensis larva (Neuroptera: Chrysopidae ) on a semisolid artificial diet. J. Environ. Entomol. 32(1): 85–89. Google Scholar
- S. Q. Li , S. S. Huang , S. C. Han , Z. G. Li , J. W. Ye , Y. J. Zhang 2011. Impact of low-temperature refrigeration on the eggs and pupal development of Mallada sp. J. Environ Entomol. 33(4): 478–481. Google Scholar
- S. Q. Li , S. S. Huang , S. C. Han , Z. G. Li , J. W. Ye , and Y. J. Xu 2012. Functional and numerical responses of Mallada basalis feeding on Corcyra cephalonica eggs. Acta Ecol. Sinica 32(21): 6842–6847. Google Scholar
- L. P. Lounibos , R. L. Escher , D. Duzak , and E. A. Martin 1996. Body size, sexual receptivity and larval cannibalism in relation to protandry among Toxorhynchites mosquitoes. Oikos 77: 309–316. Google Scholar
- M. U. Muhammad , S. Abdus , S. Zahoor , F. Abid , U. Amjad , and U. K. K. Sana 2006. Effect of different artificial diets on the biology of adult green lacewing (Chrysoperla carnea Stephens). Songklanakarin J. Sci. Technol. 28(1): 1–8. Google Scholar
- J. A. Newman , and M. A. Elgar 1991. Sexual cannibalism in orb-weaving spiders: An economic model. American Nat. 138: 1372–1395. Google Scholar
- G. A. Polis , C. A. Myers , and R. Holt 1989. The evolution and dynamics of intraguild predation between potential competitors. Annu. Rev. Ecol Sys. 20: 297–330. Google Scholar
- S. B. Qiu 1997. Green lacewing rearing in winter. Entomol. Knowledge (5): 143–144. Google Scholar
- R. L. Ridgeway , R. K. Morrison , and M. Badgley 1970. Mass rearing a green lacewing. J. Econ. Entomol. 63(3): 834–836. Google Scholar
- F. Samu , S. Toft , and B. Kiss 1999. Factors influencing cannibalism in the wolf spider Pardosa agrestis (Araneae, Lycosidae). Behav. Ecol. Sociobiol. 45: 349–354. Google Scholar
- R. D. Semlitsch , and C. A. West 1988. Size-dependent cannibalism in noctuid caterpillars. Oecologia (Berl) 77: 286–288. Google Scholar
- P. Sowing 1997. Predation among Sphaeridium larvae: The role of starvation and size difference (Coleoptera: Hydrophilidae). Ethol. Ecol. Evol. 9: 241–251. Google Scholar
- J. W. Su , and C. F. Sheng 1999. Biology of the green lacewing (Chrysopa phyllochroma ). 1: Effective accumulated heat and development rate. Entomol. Sinica 6(3): 277–282. Google Scholar
- S. Wang , X. L. Tan, and F. Zhang 2010. Influence of kin relationship on cannibalism behavior of Harmonia axyridis (Coleoptera: Coccinellidae) fourth instar larvae in different temperature conditions. Acta Ecol. Sinica 30(19): 5396–5403. Google Scholar
- D. H. Wise 1993. Spiders In Ecological Webs. Cambridge University Press, Cambridge. Google Scholar
- D. H. Wise 2006. Cannibalism, food limitation, intraspecific competition and the regulation of spider populations. Annu. Rev. Entomol. 51: 441–465. Google Scholar
- H. F. Xu , Y. Liu , and J. Y. Mou 1997. Functional Response of Chrysopa septempunctata and Chrysopa phyllochroma on Aphis citricola. J. Shandong Agric. Sci. (6): 28–30. Google Scholar
- J. W. Ye , L. M. Huang , Z. G. Li , and S. C. Han 2012. Life table of experimental population of Mallada sp. feeding on Corcyra cephalonica (Stainton) and Pseudococcidae. Chinese J. Biol. Control 28(2): 289–292. Google Scholar
- J. W. Ye , Z. G. Li , X. Lv , Q. Guo , L. Jiang , and S. C. Han 2013. Functional and numerical reponses of Mallada basalis (Walker) larvae on Icerya aegyptiaca (Douglas) nymphs. J. Environ. Entomol. 35(1): 67–71. Google Scholar
- F. Zhang , S. Q. Wang , C. Luo , Y. H. Chen , and F. Li 2004. Effects of artificial diets and breeding means on growth and development of Chrysopa septempunctata Wesmael. Plant Prot. 30(5): 36–40. Google Scholar