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20 July 2010 Genetic population structure of an autumn-emerging caddisfly with inherently low dispersal capacity and insights into its phylogeography
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We used ecological information and current and historic species distribution models for the last glacial maximum (LGM) to develop hypotheses regarding the Pleistocene history of the montane, autumn-emerging caddisfly Chaetopterygopsis maclachlani. We used mitochondrial sequence data from the cytochrome oxidase I (mtCOI) gene of 282 specimens from populations across Europe to analyze the population structure and phylogeography of C. maclachlani and to test our hypotheses. We examined the population genetic structure with median-joining networks, analysis of molecular variance (AMOVA), exact tests of population differentiation, and Mantel tests of isolation by distance. Furthermore, we used tests for selective neutrality (Tajima's D, Fu's FS) to infer potential population growth and expansion and Bayesian Skyline Plots to analyze past population dynamics. We found strong population structure with 47 different haplotypes that could be separated into a southeastern and a western clade. The western clade seems to have survived at least the LGM in an extra-Mediterranean refugium, independent of the southeastern clade. Within the 2 main clades, we found haplotype overlap between mountain ranges and a high proportion of private haplotypes (89%). As predicted for a montane species with very limited adult dispersal capabilities, many regions and populations are currently isolated and appear to be diversifying into independent lineages. We discuss speciation within the Chaetopterygini and the diversification of C. maclachlani in particular.

The occurrence of a species and the distribution of its genetic variation result from a species response to historic habitat contraction and expansion, e.g., from past climate change and the location, number, and size of refugia, and present environmental conditions, dispersal capability, and connectedness of populations (Hewitt 2004, Schmitt 2007, Hughes et al. 2009). Although the patterns of species responses to glacial cooling currently are understood only for individual species, a growing consensus is that most terrestrial temperate species in central Europe must have survived glaciations in ≥1 refugia in more moderate climates in southern Europe (Hewitt 2004, Schmitt 2007). Some cold-tolerant or cold-hardy species were able to remain in place and survived glaciations in nunataks or cryptic northern refugia in the periglacial zone between northern and southern glacial expanses (Stehlik et al. 2002, Pauls et al. 2006, Mráz et al. 2007, Naciri and Gaudeul 2007). During interstadials, interglacials, or after glaciation, recolonization of central Europe started from these refugia (reviewed by Stewart et al. 2009). Stream insects with an aquatic larval stage and a terrestrial adult stage are interesting subjects for phylogeographic investigations because of their different dispersal pattern and the distinct linear structure of stream habitats.

The winged adult stage of aquatic insects often is considered the stage in which the main lateral dispersal among streams, catchments, or over long distances can occur (Bilton et al. 2001, Petersen et al. 2004, Hughes 2007). However, many aquatic insects inhabit large ranges but have brachypterous (short-winged) or apterous (wingless) adults, or emerge during cold periods of the year when dispersal generally is assumed to be poor. In caddisflies, brachyptery is rare (Giudicelli and Botoşaneanu 1999, Holzenthal et al. 2007, Graf et al. 2009). Moreover, most aquatic insect species of temperate regions emerge in summer when dispersal capacity can be considered highest for poikilothermic animals. Some exceptions are winter-emerging stoneflies (e.g., Allocapnia spp., Ross and Ricker 1971), caddisflies (e.g., Melampophylax mucoreus, Waringer and Graf 1997), and dipterans (e.g., Bryophaenocladius thaleri, Willassen 1996; Diamesa mendotae, Bouchard and Ferrington 2009). Thus, caddisflies of the Limnephilidae tribe Chaetopterygini present an interesting intermediate form: adults of these species emerge in autumn, are brachypterous with relatively short, rounded wings, and are poor fliers or are unable to fly (Botoşaneanu 1975, Wagner 1991, Malicky 1994). Like most autumn-emerging caddisflies, they generally are not seen flying, but rather are seen crawling through riparian vegetation and are rarely caught with light traps (Malicky 1987, Malicky and Krušnik 1991).

Many species of autumn-emerging Chaetopterygini caddisflies are endemic to single mountain ranges or small areas (Malicky 1994). However, other species are widespread across Europe. One such species is Chaetopterygopsis maclachlani Stein 1874 (Fig. 1A, 2). Disjunct insular highland or montane distributions can result either from past fragmentation of a previously broader, more coherent distribution range or from repeated long-distance dispersal events and founding of new populations in isolated patches of suitable habitat. Our first hypothesis is that short-winged, autumn-emerging caddisfly species should exhibit stronger genetic population structure than other montane caddisfly species that emerge in summer or are good fliers.

Figure 1

Climatic niche model predictions for Chaetopterygopsis maclachlani. The range of the suitable climatic niche (black shading) is shown at present (A) and during the last glacial maximum (LGM) based on the Community Climate System Model (CCSM) (B) and the Model for Interdisciplinary Research on Climate (MIROC) (C). See Appendix 1 for details.


We followed the approach advocated by Richards et al. (2007) and used our genetic data to test 2 additional phylogeographic hypotheses derived from last glacial maximum (LGM) climate-niche models (Fig. 1B, C, Appendix 1). First, in line with the LGM climate niche distribution under the Community Climate System Model (CCSM) model (Fig. 1B) we hypothesized that C. maclachlani persisted in 2 independent refugial areas, 1 in western and central Europe and 1 east of the present-day Adriatic Sea. Under this scenario, we would expect to see a deep split between eastern and western European populations. The Model for Interdisciplinary Research on Climate (MIROC) offers an alternative scenario to this hypothesis. Under this model, C. maclachlani survived in a more-or-less contiguous metapopulation across central and eastern Europe (Fig. 1C). Under this scenario, we would predict shallow divergence and little genetic differentiation among central and eastern European populations of C. maclachlani. Second, we hypothesized that C. maclachlani was more widespread during the LGM and is currently a regressive species with a fragmented distribution, restricted to colder habitats, i.e., higher elevations. This scenario is consistent with both LGM niche models and should result in a pattern of demographic regression after the LGM.

We used mitochondrial cytochrome oxidase I (mtCOI) sequence data to examine the population structure and demographic history of the widespread autumn-emerging caddisfly C. maclachlani across its main distribution range in Europe. We explicitly tested the hypotheses outlined above. Our study allowed us to examine how species with low inherent dispersal capacity can occupy large ranges that were climatically dynamic in recent history. We predicted that because of the species' discontinuous distribution in cold headwater mountain streams and its low inherent dispersal capacity, which is a key ecological factor influencing the structure of a population (Hughes 2007), C. maclachlani would exhibit strong genetic population structure, indicative of long-term persistence in the vicinity of its current area of distribution.


Study species

We chose Chaetopterygopsis maclachlani Stein, 1874 (Trichoptera∶Limnephilidae∶Chaetopterygini) as a model species with which to examine how a species with intermediate dispersal capacity could occupy a large range in a historically dynamic landscape. The genus Chaetopterygopsis consists of 3 Eurasian species. Chaetopterygopsis maclachlani has 2 sister taxa in eastern Europe: Chaetopterygopsis sisestii Botoşaneanu, 1961 in the Carpathians and Chaetopterygopsis siveci Malicky, 1988 in Greece (Graf et al. 2008). Chaetopterygopsis maclachlani is distributed across western and central Europe and extends to the Ural and Baikal region in the east.

The larvae of C. maclachlani live in the springs, headwaters, and middle reaches of streams (eucrenal to metarhithral) (Graf et al. 2008). Thus, larvae occur over a large stretch of streams and have been observed 0.3 to 50 km from the spring (Pitsch 1993). Our observations showed that larvae often are found in patches of moss of the genus Fontinalis, usually in areas of the stream with slower current or laminar flow. In the Vosges Mountains, larvae of C. maclachlani feed on bryophytes (64.5%) and to a lesser extent on coarse leaf detritus (35.5%), indicating a very specialized feeding behavior (Dangles 2002). Chaetopterygopsis maclachlani larvae almost always build their cases using leaves from Fontinalis mosses (Malicky 1994), and they seldom use other suitable organic material, such as pieces of plant leaves (Pitsch 1993, Waringer and Graf 1997). The case is almost always longer than the body and can be >2× the body length of the larvae in the later instars (SL, SUP, personal observation). Thus, the species also seems to be very specialized in its case-building. In addition, C. maclachlani is thought to have a preference for higher altitudes or regions that are close to higher mountain ranges (Pitsch 1993, Haase 1999, Graf et al. 2008), presumably because of a preference for colder water temperatures.

Population sampling

We sampled the central distribution range of C. maclachlani and examined a total of 282 specimens from 28 populations in 12 regions (Fig. 2, Appendix 2). Chaetopterygopsis maclachlani also has been recorded from the Pyrenees, the Jura, and southwestern Alps in the west, and in northern Poland, the Dinarids, the Ural, and the Baikal area in the east (Fig. 2) (Kumanski 1988, Mey 1991, Czachorowski et al. 1993, Medvedev 1998, Graf et al. 2008). Given the autumn emergence of the species, some regions probably are not as well studied as others, e.g., France and the former Soviet Union (Kumanski 1980, Malicky 2005). Presumably, the species also occurs in the western Alps, but we were unable to find any individuals there or in the Jura Mountains during several sampling trips. The main distribution range of C. maclachlani seems to be in the eastern parts of the European Highlands where we collected many specimens (Fig. 2).

Figure 2

Sampling sites (triangles, color-coded as in Fig. 3) and European distribution (hatched area on inset) of Chaetopterygopsis maclachlani. Details on sampling sites given in Appendix 2. The European distribution of C. maclachlani is based on literature, personal communications with specialists, and the authors' observations (Kumanski 1988, Mey 1991, Czachorowski et al. 1993, Pitsch 1993, Medvedev 1998, Haase 1999, Graf et al. 2008). HZ  =  Harz, SM  =  Sudety Mountains, TF  =  Thuringian Forest, BM  =  Bohemian Massif, PW  =  Pfaelzer Wald, TA  =  Tatra, AP  =  Apuseni, RZ  =  Retezat, BF  =  Black Forest, VM  =  Vosges Mountains, AL  =  Alps, RM  =  Rila-Rhodope Massif.


Larval and adult specimens were sampled with hand nets and were stored in ethanol until deoxyribonucleic acid (DNA) extraction. Larvae and adults were identified with keys in Waringer and Graf (1997) and Malicky (2004), respectively. Vouchers are deposited at the Senckenberg Research Institute and Natural History Museum, Frankfurt, Germany.

DNA extraction, amplification, and sequencing

DNA extraction and polymerase chain reaction (PCR) amplification of the mtCOI fragment followed methods published in Lehrian et al. (2009). Primers were Dave (5′-AGTTTTAGCAGGAGCAATTACTAT-3′, position 2059 at 3′ end relative to Drosophila yakuba) and Inger (5′-AAAAATGTTGAGGGAAAAATGTTA-3′, position 2735 at 3′ end relative to D. yakuba) (Zhang and Hewitt 1996). Sequences were generated with the PCR primers by 2 commercial sequence services: Nano+Bio Center Kaiserslautern, Germany, and AGOWA GmbH Berlin, Germany.

Electropherogram traces were aligned, checked, and manually edited using Sequencher software (version 4.8; Gene Codes Corporation, Ann Arbor, Michigan). We used Basic Local Alignment Search Tool (BLAST) (Altschul et al. 1997) to verify the identity of sequences. Sequences were aligned using CLUSTAL W with default parameters as implemented in BioEdit (Hall 1999).

Data analysis

Haplotype distribution

We used phylogenetic tree- and network-building techniques to visualize the relationships among haplotypes and their distribution. The sequence alignment was imported into DnaSP 4.50.2 (Rozas et al. 2003) to generate a haplotype matrix for calculating a median-joining (MJ) haplotype network (Bandelt et al. 1999) in Network (Fluxus Technology, Suffolk, UK) using default settings. In addition, we constructed a minimum spanning network of haplotypes with 95% plausible parsimony links using TCS 1.21 (Clement et al. 2000).

We used Modeltest (Posada and Crandall 1998) to select the best-fitting model for our data. The Hasegawa-Kishino-Yano + gamma + invariant sites (HKY + Γ + I) model was chosen based on the likelihood ratio test criterion in Modeltest. We calculated a Bayesian Metropolis-Coupled Markov-Chain Monte-Carlo (B/MCMCMC) phylogenetic inference of the 47 haplotypes based on the selected model using MrBayes 3.1 (Ronquist and Huelsenbeck 2003). Four chains were run for 5 × 106 generations. Trees were sampled every 1000th generation; the first 2.5 × 106 generations were discarded as burn-in.

Genetic population structure and differentiation

Analysis of molecular variance (AMOVA) was calculated in Arlequin 3.11 (Excoffier et al. 2005) with 16,000 permutations. The distance applied was Kimura-2-Parameter distance. Our a priori grouping allowed us to test variation within vs among regions. We calculated exact tests of population differentiation (ETPD) (Raymond and Rousset 1995) and pairwise FST values as implemented in Arlequin 3.11 (Excoffier et al. 2005) to analyze differentiation between regions. We ran exact tests with 10,000 dememorization steps and a Markov Chain with a length of 100,000. We ran FST with 16,000 permutations. Significance of both was Benjamini–Yekutieli (B–Y) adjusted (Narum 2006). We applied a Mantel test to the matrices of pairwise FST values and geographical distances between all analyzed populations to assess isolation-by-distance in Arlequin 3.11 (Excoffier et al. 2005). We ran 10,000 permutations. We calculated geographic distances using ArcView® (ESRI ArcView 3.2, Redlands, California). We used Barrier 2.2 (Manni et al. 2004) to detect barriers to gene flow based on Monmonier's (1973) maximum difference algorithm. Barriers are computed associating a pairwise FST-distance matrix with a geographic data matrix.

Genetic population diversity

Gene diversity, nucleotide diversity, and pairwise differences were calculated in Arlequin 3.11 using the default settings (Excoffier et al. 2005). More recently colonized regions are expected to have lower genetic diversity than refugial areas or older areas of occurrence because an expanding population contains only a subset of the haplotypes found in the ancestral population (Ibrahim et al. 1996).

Demographic history and population expansion

To test for recent demographic expansion in each region and in the whole data set, we calculated Tajima's D and Fu's FS in Arlequin 3.11 (Excoffier et al. 2005) with 16,000 permutations. These metrics are expected to have significantly negative values if the populations are not in equilibrium between mutation and drift, e.g., because of demographic expansion. These negative values can arise under selective effects, but they can also be indicative of population expansion or bottlenecks (Tajima 1993a, 1996). Fu's FS is especially sensitive to population expansions (Fu 1997).

Past population dynamics were analyzed using Bayesian Skyline Plots (BSP; Drummond and Rambaut 2007) implemented in BEAST 1.5.3 (Drummond et al. 2005). BSPs were calculated separately for the eastern and western clades of C. maclachlani and for the total data set containing all individual sequences. The relative rate test (Tajima 1993b) in MEGA 4.0 did not allow us to reject the hypothesis that our sequences were evolving in a clock-like manner. We used a divergence rate of 2.3%/my (106 y; Brower 1994) under the relaxed uncorrelated log-normal model. We chose a piecewise-constant skyline model for the analyses. We constructed an Unweighted Pair Group Method with Arithmetic mean (UPGMA) starting tree before each run. We ran tests for 100 million generations for each clade and the whole data set to ensure high effective sample sizes (ESS). We sampled chains every 10,000 states and removed 10% of the samples as burn-in. We did 2 independent runs for the eastern clade and 4 runs for the western clade and the complete data set. We combined the results of the independent runs in the program LogCombiner (version 1.4.8; to check the convergence and mixing of independent chains. We created BSPs in Tracer (version 1.4.1; based on the combined results.


The final alignment was 497 base pairs (bp) long and contained 46 variable positions, which resulted in 47 unique haplotypes (GenBank accession numbers: HM005165–HM005211). The nucleotide composition showed the typical adenine thymine (AT)-rich signature of insect mitochondrial DNA (cytosine [C]: 18.13%, T: 41.02%, A: 24.88% and guanine [G]: 15.97%).

Haplotype distribution

We visualized geographic distribution of the 47 identified haplotypes by color coding in a MJ network (Fig. 3). The MJ and the TCS network were similar. The TCS network connected all haplotypes in 1 network with 95% confidence, but it built 1 loop more than the MJ network between H18 and H32 (red dotted line in Fig. 3). Maximum divergence between all haplotypes was 3.82% and the mean pairwise difference was 4.591 ± 2.262 bp. Two main clades were identified. Clade 1 (top) consisted of 33 haplotypes from the northern Carpathians and central Europe, whereas clade 2 (bottom) represented a southeastern lineage with 14 haplotypes. The clades were divergent by 6 mutational steps (p  =  1.21%). Clade 1 was characterized by 2 central haplotypes (H1, H8), that differed by 1 bp, each of which was connected to a number of haplotypes by 1 to 3 bp changes. Central haplotype H8 (N  =  75) and haplotypes connected to it were associated with the Bohemian Massif, Alps, Sudety Mountains, or Harz. Central haplotype H1 (N  =  39) and its connected haplotypes were associated with the Black Forest, Pfaelzer Wald, and Vosges Mountains in the west and the Thuringian Forest, Sudety Mountains, and Tatra in the east. Most haplotypes were private, i.e., specific to a single region (Fig. 3).

Figure 3

Median-joining network of Chaetopterygopsis maclachlani haplotypes. Color-coding corresponds to origin of the specimens carrying a given haplotype. Circle diameter is relative to number of specimens carrying a haplotype. Hatch marks or numbers on connecting lines indicate number of base pair (bp) changes between haplotypes. If no number is shown, the number of bp changes is 1. The red dashed line shows the additional loop found in the network generated with TCS (Clement et al. 2000).


In clade 2 (southeastern lineage), 1 central haplotype (H5; N  =  53) was present in all of the sampled mountain ranges in the region (Apuseni, Rila/Rhodope Massif, and Retezat Mountains). About ½ of the other haplotypes in haplogroup 2 were singletons that diverged by 1 or 2 bp from H5. The remaining singleton haplotypes were connected to H5 by ≤6 bp. H21 was the only nonsingleton haplotype, was connected to H5 by 1 bp and was endemic to the Rila/Rhodope Massif.

Several regions exhibited relatively high levels of haplotype endemism. The Tatra Mountains had 7 private haplotypes (H26, H37, H39, H40, H46, H45, H47), 3 of which were not singletons. The Harz had 5 private haplotypes (H6, H7, H27, H28, H29), but only 1 of these was not a singleton. The Thuringian Forest had only a single private haplotype (H41). In total, 42 of 47 haplotypes (89%) were private to a single region.

The unrooted Bayesian 50% majority-rule consensus tree also showed that the 47 haplotypes clustered into 2 strongly supported clades (a southeastern clade and a western clade) with posterior probability  =  1.00. The topology showed the same relationships among haplogroups and haplotypes as the 2 haplotype networks and, therefore, is not shown.

Genetic population structure and differentiation

To avoid analytical issues with low sample sizes for some populations (SM2, BM1, BM2, BM8, PW, AP1, BF1), we decided not to analyze the data at the population level, but rather at the regional level. All specimens from distinct mountain ranges that were separated by valleys of lower altitude (<400 m asl) or other clear geological/orographic breaks (e.g., between Thuringian Forest and Bohemian Massif) were grouped into region/mountain range groups. This grouping is nonrandom but is based on the species preference for higher altitudes and its naturally insular distribution pattern.

AMOVA partitioned variation in our specimens among regions, among populations within regions, and within populations based on our a priori groupings. Most of the molecular variation observed in our data set could be attributed to variation among regions (85.79%, ΦCT  =  0.858, p < 0.001), whereas very little variation occurred among populations within regions (1.37%, ΦSC  =  0.096, p  =  0.005). Variation within populations accounted for 12.85% (ΦST  =  0.872, p < 0.001) of the total variation.

High levels of differentiation between regions were observed in ETPD and pairwise FST values. ETPD revealed significant differentiation in 54 of 66 comparisons (82%) and pairwise FST values were significant for 57 of 66 comparisons (86%) after B–Y adjustment (α  =  0.01037) (Table 1). Populations in the Bohemian Massif and the Alps were significantly differentiated from populations in all other regions in both tests. In both tests, populations in the Vosges Mountains, the Black Forest, the Harz, the Thuringian Forest, the Sudety Mountains, and the Tatra also were significantly differentiated from populations in all other regions except from the Pfaelzer Wald, presumably because of small sample sizes at the region scale (n  =  2). We detected 9 barriers to gene flow (Barrier 2.2). The 1st barrier was between the Pfaelzer Wald populations and all other populations, presumably because of the limited sample size. The 2nd barrier was between the Bohemian Massif and the Sudety Mountains, regions that split our samples into eastern and western groups. The 3rd barrier was detected between the Tatra and the Apuseni Mountains and coincided with the 2 main clades in the haplotype network. The probability associated with each remaining barrier decreased, and barriers 4 to 9 merely provided finer splitting of distinct geographic regions.

Table 1

Pairwise FST-values (bold indicates statistical significance). Significant results of exact tests for differentiation between regions are indicated with +. Significance level after Benjamini–Yekutieli adjustment for both tests is α  =  0.01037.


The Mantel test revealed significant isolation by distance (correlation coefficient r  =  0.318, p < 0.01) in the whole data set. We used populations instead of regions to test for isolation by distance because use of geographic distance based on the geographic center of each region could falsify the correlation between geographic and genetic distance, especially in regions, such as the Bohemian Massif, where samples were widely separated. When clades were analyzed independently, an isolation-by-distance signal was observed in the western clade (r  =  0.286, p < 0.01), but not in the southeastern clade (r  =  0.032, p > 0.05). Therefore, we can infer that the western clade was comparatively closer to equilibrium between genetic drift and gene flow than the southeastern clade.

Genetic population diversity

Gene diversity was higher in the western lineage (0.821 ± 0.019) than in the southeastern lineage (0.376 ± 0.077) as a result of greater divergence between haplotypes and a larger number of haplotypes. Gene diversity of all individuals was 0.862 ± 0.012, but the nucleotide diversity was only 0.100 ± 0.054, and the mean number of pairwise differences was 4.591 ± 2.262 (Table 2). Gene diversity was highest among individuals in the Pfaelzer Wald, the Harz, the Tatra, and the Black Forest. How genetically diverse the Pfaelzer Wald truly is remains in question because the total sample from the region contained only 2 specimens. We found 5 different haplotypes in 6 individuals in the Harz, whereas we found only 1 haplotype in 6 individuals in the Thuringian Forest (H41) and 1 haplotype in 5 individuals in Retezat (H5) populations. The difference among these 3 populations seems clear, but low sample sizes make it difficult to come to strong conclusions. We also found high numbers of haplotypes/individual in the Tatra Mountains and Black Forest (8 haplotypes in 20 individuals, and 7 haplotypes in 17 individuals, respectively). We found a high number of haplotypes/individual in the Apuseni Mountains (7 haplotypes in 15 individuals), but gene diversity was relatively low (0.657 ± 0.138).

Table 2

Gene diversity, nucleotide diversity, and mean number of pairwise differences for each region. See Table 1 for region codes. n.a.  =  not available.


Demographic history and population expansion

For the whole data set, Fu's FS indicated a recent demographic change (FS  =  −21.529, p  =  0.00025). Tajima's D also was negative, a result indicating demographic change, but was not significant for the whole data set (D  =  −1.088, p  =  0.10956; Table 3). At the regional scale, both Tajima's D and Fu's FS were significantly negative for the Bohemian Massif (D  =  −1.892, p < 0.05; FS  =  −5.468, p < 0.01) and the Rila Rhodope Massif (D  =  −2.251, p < 0.01; FS  =  −4.902, p < 0.01), results indicating recent demographic expansion of C. maclachlani in these regions. The Harz populations had negative values in both tests, but only Fu's FS was significant (FS  =  −3.047, p  =  0.003; D  =  −1.295, p  =  0.072).

Table 3

Results of neutrality tests (Fu's FS and Tajima's D) by region and the total data set. See Table 1 for region codes. Bold values are significant. Fu's FS is significant at p < 0.02, Tajima's D is significant at p < 0.05. n.a.  =  not applicable.


BSPs indicated demographic expansion of the eastern and western clades, and of both clades combined shortly after the end of the Riss glaciation (Fig. 4). The higher and lower 95% highest probability density (HPD) values showed broad variations in all 3 clades, presumably because of the limited resolution of the single locus data.

Figure 4

Demographic changes of C. maclachlani populations estimated by Bayesian Skyline Plots. A.—Climatic oscillations presented as temporal change in benthic δ18O‰ isotope records of Lisiecki and Raymo (2005) plotted as the moving average of the data through time. B–D.—Mean and 95% confidence intervals (gray shading) for effective sample size (Ne) for the eastern clade (B), western clade (C), and total data set (D) plotted through time. The demographic changes reflected by the graphs are estimated back in time to the mean timing of the coalescent event. LGM  =  last glacial maximum.



Pleistocene persistence in 2 regions

The major split between western and southeastern populations of C. maclachlani is consistent with the 2-refugia hypothesis derived from the CCSM and is inconsistent with the alternative common refugia hypothesis based on the MIROC (Fig. 1B, C). Use of standard molecular clock rate estimates is much debated, and the standard rate of 2.3%/my can greatly underestimate true diversification rates (e.g., Gratton et al. 2008). We do not have paleontological or paleo-ecological benchmarks for calibrating our mutation rate, so we do not wish to overemphasize the dating of our results. However, based on both the conventional rate of 2.3%/my and a 10× higher rate suggested for recent diversification in the Clouded Apollo (Parnassius mnemosyne) (Gratton et al. 2008), the southeastern and western clade would have split well before the LGM. All haplotypes we observed in C. maclachlani differed by 1 to 19 mutational steps. Maximum divergence between all haplotypes was 3.82%, which would place the split between 165 ky (103 y) to 1.65 my ago with the above rates. The eastern clade would coalesce to ∼200 ky or 2 my with the 10× rate. No haplotype overlap was found, so the species probably persisted in independent refugia for ≥1 glacial cycle, a result that provides further support for the 2-refugia scenario predicted under the CCSM LGM climate scenario. This persistence in independent refugia would have given rise to the southeastern and western clades. Other European caddisfly species also show diversification events originating in the late Pliocene (Drusus discolor; Pauls et al. 2006), through the Pleistocene (Drusus bosnicus-group, D. croaticus, Previšic et al. 2009), and into the Holocene (R. pubescens, Engelhardt et al. 2008).

Geographically, the split lies between the Tatra Mountains in the Western Carpathians and the Apuseni and Retezat Mountains in the Southern Carpathians. The break we observed in C. maclachlani is common in plants that inhabit the Tatra Mountains and the rest of the Carpathians. This phytogeographical boundary between the Eastern Carpathians and Tatra Mountains was first recognized by Woloszczak in 1896 (Ronikier et al. 2008). Large genetic divergence between Tatra populations and populations from the rest of the Carpathians is reported for some plants (Mráz et al. 2007, Ronikier et al. 2008, Tollefsrud et al. 2008, Liepelt et al. 2009) and, to some extent, the bank vole (Kotlík et al. 2006). For aquatic insects, the only genetic studies known to us that sampled across this region were the D. discolor studies by Pauls et al. (2006, 2009). In both cases, haplotype overlap was found between the regions and divergence was limited to 2 bp among the Carpathian and Tatra Mountain clades. However, Botoşaneanu (1975) found a morphology-based speciation gradient that was related to features of the female and male genitalia for Annitella species (Chaetopterygini) along the main axis of the Carpathians. He assumed that Annitella originated from the Balkans and colonized the Carpathians and the central European Highlands gradually after the Würm glaciations. Another Chaetopterygini, Psilopteryx psorosa, can be separated into regional subspecies along a geographic gradient in the Carpathians and the adjoining mountain ranges (Mey and Botoşaneanu 1985). Unfortunately, we cannot fully resolve the genetic break between the Eastern Carpathians and the Tatra Mountains because we could not find any individuals during our sampling campaign in the Eastern Carpathians.

Centers of high diversity often are regarded as refugial regions because they harbor much of the genetic diversity from which newly colonized regions take their source. Newly founded populations frequently have signals of reduced genetic diversity through founder effects or bottlenecks until they reach equilibrium (e.g., Ibrahim et al. 1996). The Carpathian basin, including the Apuseni Mountains, often has been considered a glacial refugium and a genetic center (Botoşaneanu 1975, Deffontaine et al. 2005, Sommer and Benecke 2005, Malicky 2006, Pauls et al. 2006, 2009, Ursenbacher et al. 2006, Bálint et al. 2008). The refugial sources for the southeastern lineage in the more southern Retezat and the Balkans could have been in the Apuseni. As expected for refugia, gene diversity is higher in the Apuseni than in the other southeastern lineage regions. This hypothesis also is consistent with H5 as the central and presumably ancestral haplotype that can be found in all of the southeastern ranges. As is typical for recent haplotypes, only haplotypes that have few base pair changes and are at the tips of the network are derived from the putative ancestral haplotype H5. An alternative hypothesis is that genetic diversity is high in the Apuseni Mountains because they support multiple local refugial populations. Under this scenario, the Southern Carpathians and the Balkan Mountains could have been recolonized from a common refugial source south of the Carpathians. This scenario is consistent with the CCSM LGM distribution, which indicates that suitable source areas existed in the vicinity of the western Balkan Mountains in present-day Bulgaria and the Banat Mountains in southwestern Romania. Recent disjunction between the Carpathians and the Balkan Mountains and signs of recolonization from a Pleistocene refugia south of the Carpathians have been identified in the case of Erebia euryale (Schmitt and Haubrich 2008). This hypothesis also is consistent with the current distribution of haplotype H5.

In the western clade, high levels of diversity and the presence of 2 central ancestral haplotypes (H1 and H8) indicate recent migration and colonization events from very recently split lineages. Haplotype H1 was observed in the Sudety Mountains and Tatra Mountains in the east and the Vosges Mountains, Pfaelzer Wald, and Black Forest in the west. The Thuringian Forest population presumably also was descendent from the H1-associated refugial source. Thus, these regions and, presumably, the regions in between are remnants of a once more widespread lineage of C. maclachlani. Based on the current distribution, it appears that H1 became extinct in the intermittent region between the Thuringian Forest and the Black Forest. Whether the extinction was a consequence of competition or a climate change event cannot be resolved. The intermittent region is now occupied by a different, recently split lineage derived from H8. This lineage might have been from a 2nd independent refugial source or might have been a postglacially fragmented lineage associated with the Eastern Alps, Bohemian Massif, and the Harz. The lack of intermediate populations, e.g., in the Thuringian Forest, that carry haplotypes probably derived from H8 is inconsistent with a migratory movement from north to south or south to north between the Harz and the Bohemian Massif. Therefore, we currently favor the scenario of a relatively recent fragmentation with incomplete lineage sorting among the 2 central European subclades (see below). However, we were able to sample only a single population in the Thuringian Forest. Therefore, this potential secondary contact zone might not be sufficiently sampled to draw conclusions because alternative migratory pathways might have existed along the Thuringian Massif.

Postglacial population dynamics

Given the poor dispersal capacity of autumn-emerging caddisflies in general and of C. maclachlani in particular, fragmentation of a more cohesive distribution range seems to be a likely explanation for the high level of population differentiation within the western clade. Chaetopterygopsis maclachlani is a cold-tolerant species and might have occupied a wide range at lower elevations during cooler periods in the past as predicted by both climate niche models (Fig. 1B, C). This period of broad distribution could have been followed by extinction of several populations between mountain ranges during warmer phases. At the same time, some individuals could have survived by moving to higher altitudes. This scenario of shifts to lower altitudes at cooler stages in the past is inferred for many cold-adapted and alpine species (Volckaert et al. 2002, Muster and Berendonk 2006, Schmitt et al. 2006).

Our results suggest that C. maclachlani experienced demographic expansion in all parts of its range. The standard substitution rate (2.3%/my) used to estimate the timing of population events indicates that these expansions coincided with the end of the Würm glaciation, but the general scenario is independent of the method for calculating dates (standard or otherwise-calibrated molecular clock). The mean estimated effective population size of the western lineage also suggests that this long phase of population expansion was followed by a very recent decline shortly after the end of the Würm glaciation. It is plausible that C. maclachlani had a widespread, cohesive distribution throughout some cold periods of the Pleistocene before being split into 2 main refugial areas. In response to a warming postglacial climate, the species retreat to higher elevations caused fragmentation and forced the species into headwater systems. In a few regions, this process might have gone hand in hand with a demographic expansion if less cold-tolerant competitors remained downstream. In most regions, the isolated headwater populations would be experiencing a range regression. The main split into a southeastern and a western clade, and a very recent demographic decline in western clade is consistent with such a scenario.

Diversification of autumn-emerging caddisflies

Adult dispersal is the main source for gene flow among populations of aquatic insects (e.g. Bilton et al. 2001, Hughes 2007, Hughes et al. 2009). Our study revealed strong population structure and genetic differentiation within and among mountain ranges and populations of C. maclachlani. Clear patterns of genetic population structure caused by limited gene flow are common in insects that inhabit high montane regions and sky islands or in species with other types of fragmented distributions (e.g., Mardulyn 2001, Engelhardt et al. 2008). Our results agreed with our prediction that we would find strong genetic differentiation among populations and regions of C. maclachlani, presumably because of its poor flying capabilities, and thus, limited lateral dispersal capacity. Nevertheless, C. maclachlani was able to colonize a very wide range in central Europe, presumably during the glacial stages of the Pleistocene, and is now retreating to higher altitude, isolated populations.

The degree of differentiation of C. maclachlani among regions is similar to that of other European caddisflies that are considered better fliers based on their wing shape, emergence period, and adult flight behavior (Pauls et al. 2006, 2009, Bálint 2008, Engelhardt et al. 2008). Chaetopterygopsis maclachlani populations in most regions are significantly separated from those in other regions (ETPD: 82% of pairwise tests were significant, pairwise FST: 86%). Very similar patterns were observed at the regional scale for D. discolor (ETPD: 83%, FST: 87%) and R. pubescens (ETPD: 72%) across Europe (Pauls et al. 2006, Engelhardt et al. 2008), D. romanicus (ETPD: 100%) in the Carpathians and Southern Balkan regions (Pauls et al. 2009), and the Rhyacophila aquitanica-complex (ETPD: 93%, pairwise FST: 98%) among the Massif Central, Black Forest, Vosges Mountains, Southern Alps, and the Carpathians (Bálint 2008). Eighty-nine percent of C. maclachlani haplotypes are private to a single region, compared with 83% in D. discolor, 92% in D. romanicus, and 83% in R. aquitanica (Pauls et al. 2006, 2009, Bálint 2008). Eighty-six percent of R. pubescens haplotypes are private across its range north of the Alps (Engelhardt et al. 2008). Thus, many species of caddisflies with different inherent levels of dispersal capability show high degrees of differentiation in Europe, results suggesting limited gene flow among central European mountain ranges. However, differentiation was much lower and fewer haplotypes were private in the putatively best flier, Hydropsyche tenuis (Lehrian et al. 2009). These results follow the predictions of the “Widespread gene flow with isolation by distance model” (H. tenuis) and the “Headwater model” (all other species discussed above) for genetic structure of aquatic organisms (Finn et al. 2007, Hughes et al. 2009).

Regardless how the current genetic population structure arose, many regions and populations currently appear to be isolated and diversifying into independent lineages that could potentially evolve further into subspecies or ultimately species. Given enough time, genetic and morphological differences are likely to accumulate because of C. maclachlani's poor dispersal capacity. Chaetopterygopsis maclachlani adults, like most insects, are incapable of flying great distances at very low temperatures, which are common in autumn when they emerge; females of C. maclachlani are unable to fly (Wagner 1991). Caddisfly larvae generally disperse only short distances by drifting downstream or moving upstream (Malicky 1994, Petersen et al. 2004). Such limited dispersal capacity should lead to isolation among disjunct populations, genetic drift, and ultimately to lineage diversification and speciation. Other autumn-emerging caddisflies also show evidence of recent or ongoing diversification. The species complex Chaetopteryx rugulosa Kolenati is an example of ongoing speciation. The subspecies are readily distinguishable at the edge of their distribution ranges (Malicky et al. 1986), but the different subspecies can mate and produce fertile offspring. In the center of the overlapping ranges of several subspecies, the morphologies introgress and distinction of lineages becomes very difficult. This pattern suggests a progressively evolving species complex, not established by allopatric fragmentation of populations (Malicky 2005, Malicky et al. 1986). In another closely related species complex, the Chaetopteryx villosa group, allopatric speciation seems to be the main factor driving the morphological differentiation (Malicky et al. 1986, Malicky 2005). These species also hybridize, resulting in intermediate populations in areas of contact between lineages (Malicky 2005). The patchy distribution of C. maclachlani populations and the high level of private haplotypes observed might indicate a very early stage of speciation. The genetic divergence between the 2 lineages of C. maclachlani is only 1.21%, but the largest interspecific distance between 2 other Limnephilidae, Drusus nigrescens and Drusus monticola, also was 1.21%, probably indicating recent speciation (Waringer et al. 2007). The interspecific distance between species of the Chaetopteryx villosa group can be as low as 0.44%, but generally ranges from about 5 to 8% (H. Malicky, Lunz, Austria, and SUP, unpublished data).

A morphological study of C. maclachlani adult genitalia across the range would be very interesting for determining whether the genetic divergences correlate with morphological features. Unfortunately our sampling of adults was too limited to accomplish this task. In general, the Chaetopteryginae present an interesting group of caddisflies for evolutionary studies. It would be particularly interesting to study the comparative phylogeography and population genetics of species across hybridization zones to examine species boundaries and demographic history of the group. Based on varied results observed in montane aquatic insect phylogeography to date, we can expect that these poorly studied species will have previously unknown and unexpected phylogeographic patterns, which could greatly improve our understanding of the aquatic biota's history in Europe and thus our management potential concerning cold-adapted species at risk through anthropogenic disturbances or global change.


We thank all our colleagues who supported this study by providing material and information or assisting in collection (see Appendix 2). We thank Jane Hughes (Griffith University), Robin Thomson (University of Minnesota), and 2 anonymous referees for comments that improved a previous version of this manuscript. Part of our work was done with the resources of the Computational Biology Service Unit from Cornell University, which is partially funded by Microsoft Corporation. Our study was supported financially by a dissertation fellowship and travel grant of the “Studienstiftung des deutschen Volkes” awarded to SL, a German Research Foundation (DFG) grant (HA-3431/2-1 and 2-2) to PH and SUP, the research funding programme LOEWE—Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz of Hesse's Ministry of Higher Education, Research, and the Arts, and a postdoctoral fellowship to SUP awarded by the German Academy of Sciences Leopoldina (BMBF-LPD 9901/8-169).

Literature Cited

  1. S. F. Altschul, T. L. Madden, and A. A. Schäffer . 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25:3389–3402. Google Scholar

  2. M. Bálint 2008. Pleistocene and Holocene history of Rhyacophila aquitanica (Insecta: Trichoptera) in the Carpathian Mountains, potential speciation centers. PhD Thesis, Babeş-Bolyai University, Cluj, Romania. (Available from: Google Scholar

  3. M. Bálint, P. C. Barnard, T. Schmitt, L. Ujvárosi, and O. Popescu . 2008. Differentiation and speciation in mountain streams: a case study in the caddisfly Rhyacophila aquitanica (Trichoptera). Journal of Zoological Systematics and Evolutionary Research 46:340–345. Google Scholar

  4. H. J. Bandelt, P. Forster, and A. Röhl . 1999. Median-Joining Networks for inferring intraspecific phylogenies. Molecular Biology and Evolution 16:37–48. Google Scholar

  5. D. T. Bilton, J. R. Freeland, and B. Okamura . 2001. Dispersal in freshwater invertebrates. Annual Review of Ecology, Evolution, and Systematics 32:159–181. Google Scholar

  6. L. Botoşaneanu 1975. Die endemischen Trichopteren der Karpaten. 91–103. in H. Malicky (editor). Verhandlungen des Sechsten International Symposiums über Entomofaunistik in Mitteleuropa. Junk. The Hague, The Netherlands. Google Scholar

  7. R. W. Bouchard and L. C. Ferrington . 2009. Winter growth, development, and emergence of Diamesa mendotae (Diptera: Chironomidae) in Minnesota streams. Environmental Entomology 38:250–259. Google Scholar

  8. A. V. Z. Brower 1994. Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from pattern of mitochondrial DNA evolution. Proceedings of the National Academy of Sciences of the United States of America 91:6491–6495. Google Scholar

  9. C. Ciubuc 1993. Checklist of Romanian Trichoptera (Insecta). Travaux du Muséum National d'Histoire Naturelle “Grigore Antipa” 33:1–147. Google Scholar

  10. M. Clement, D. Posada, and K. A. Crandall . 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology 9:1657–1660. Google Scholar

  11. S. Czachorowski, K. Lewandowski, and A. Wasilewska . 1993. The importance of aquatic insects for landscape integration in the catchment area of River Gizela (Masurian Lake, District, northeastern Poland). Acta Hydrobiologica 35:49–64. Google Scholar

  12. O. Dangles 2002. Functional plasticity of benthic macroinvertebrates: implications for trophic dynamics in acid streams. Canadian Journal of Fisheries and Aquatic Sciences 59:1563–1573. Google Scholar

  13. F. Deffontaine, R. Libois, P. Kotlík, R. Sommer, C. Nieberding, E. Paradis, J. B. Searle, and J. R. Michaux . 2005. Beyond the Mediterranean peninsulas: evidence of central European glacial refugia for a temperate forest mammal species, the bank vole (Clethrionomys glareolus). Molecular Ecology 14:1727–1739. Google Scholar

  14. A. J. Drummond and A. Rambaut . 2007. Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7:214. Google Scholar

  15. A. J. Drummond, A. Rambaut, B. Shapiro, and O. G. Pybus . 2005. Bayesian coalescent inference of past population dynamics from molecular sequences. Molecular Biology and Evolution 22:1185–1192. Google Scholar

  16. C. H. M. Engelhardt, S. U. Pauls, and P. Haase . 2008. Population genetic structure of the caddisfly Rhyacophila pubescens, (Pictet, 1834), north of the Alps. Fundamental and Applied Limnology 173:165–176. Google Scholar

  17. L. Excoffier, G. Laval, and S. Schneider . 2005. Arlequin version 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1:47–50. Google Scholar

  18. D. S. Finn, M. S. Blouin, and D. A. Lytle . 2007. Population genetic structure reveals terrestrial affinities for a headwater stream insect. Freshwater Biology 52:1881–1897. Google Scholar

  19. Y-X. Fu 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925. Google Scholar

  20. J. Giudicelli and L. Botoşaneanu . 1999. The remarkable case of a water striding, mainly brachypterous new species of Rhyacophila Pictet (Trichoptera) from the River Sorgue (southern France). Annales de la Société Entomologique de France (Nouvelle Série) 35:265–273. Google Scholar

  21. W. Graf, J. Murphy, J. Dahl, C. Zamora-Muñoz, and M. J. López-Rodriguez . 2008. Volume 1: Trichoptera. 1–388. in A. Schmidt-Kloiber and D. Hering . (editors). Distribution and ecological preferences of European freshwater organisms. Pensoft. Sofia, Bulgaria. Google Scholar

  22. W. Graf, S. U. Pauls, and J. Waringer . 2009. The larva of Rhyacophila ferox Graf, 2006 (Trichoptera: Rhyacophilidae) from the Eastern Alps (Carinthia, Austria). Aquatic Insects 31:111–117. Google Scholar

  23. P. Gratton, M. K. Konopinski, and V. Sbordoni . 2008. Pleistocene evolutionary history of the Clouded Apollo (Parnassius mnemosyne): genetic signature of climate cycles and a ‘time-dependent’ mitochondrial substitution rate. Molecular Ecology 17:4248–4262. Google Scholar

  24. P. Haase 1999. Zoozönosen, Chemismus und Struktur regionaler Bachtypen im niedersächsischen und nordhessischen Bergland. Ökologie und Umweltsicherung Witzenhausen 18:1–157. Google Scholar

  25. T. A. Hall 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95–98. Google Scholar

  26. G. M. Hewitt 2004. Genetic consequences of climatic oscillations in the Quaternary. Philosophical Transactions of the Royal Society of London Series B: Biological Sciences 359:183–195. Google Scholar

  27. R. J. Hijmans, S. E. Cameron, J. L. Parra, P. G. Jones, and A. Jarvis . 2005. Very high resolution interpolated climate surfaces for global land areas. International Journal of Climatology 25:1965–1978. Google Scholar

  28. R. W. Holzenthal, R. J. Blahnik, A. L. Prather, and K. M. Kjer . 2007. Order Trichoptera Kirby, 1813 (Insecta), caddisflies. Zootaxa 1668:639–698. Google Scholar

  29. J. M. Hughes 2007. Constraints on recovery: using molecular methods to study connectivity of aquatic biota in rivers and streams. Freshwater Biology 52:616–631. Google Scholar

  30. J. M. Hughes, D. J. Schmidt, and D. S. Finn . 2009. Genes in streams: using DNA to understand the movement of freshwater fauna and their riverine habitat. BioScience 59:573–583. Google Scholar

  31. K. M. Ibrahim, R. A. Nichols, and G. M. Hewitt . 1996. Spatial patterns of genetic variation generated by different forms of dispersal during range expansion. Heredity 77:282–291. Google Scholar

  32. P. Kotlík, V. Deffontaine, S. Mascheretti, J. Zima, J. R. Michaux, and J. B. Searle . 2006. A northern glacial refugium for bank voles (Clethrionomys glareolus). Proceedings of the National Academy of Sciences of the United States of America 103:14860–14864. Google Scholar

  33. K. Kumanski 1980. Chaetopterygini from the Caucasus. Reichenbachia. Staatliches Museum für Tierkunde Dresden 18:153–160. Google Scholar

  34. K. Kumanski 1988. Trichoptera, Integripalpia. Fauna Bulgarica 19:1–354. Google Scholar

  35. S. Lehrian, S. U. Pauls, and P. Haase . 2009. Contrasting patterns of population structure in the montane caddisflies Hydropsyche tenuis and Drusus discolor in the Central European highlands. Freshwater Biology 54:283–295. Google Scholar

  36. S. Liepelt, R. Cheddadi, J-L. de Beaulieu, B. Fady, D. Gömöry, E. Hussendörfer, M. Konnert, T. Litt, R. Longauer, R. Terhürne-Berson, and B. Ziegenhagen . 2009. Postglacial range expansion and its genetic imprints in Abies alba (Mill.) – a synthesis from palaeobotanic and genetic data. Review of Palaeobotany and Palynology 153:139–149. Google Scholar

  37. L. E. Lisiecki and M. E. Raymo . 2005. A Pliocene–Pleistocene stack of 57 globally distributed benthic δ18O records. Paleoceanography 20:PA1003. Google Scholar

  38. H. Malicky 1987. Anflugdistanz und Fallenfangbarkeit von Köcherfliegen (Trichoptera) bei Lichtfallen. Jahresberichte der Biologischen Station Lunz 10:140–157. Google Scholar

  39. H. Malicky 1994. Die Chaetopterygini (Insecta, Trichoptera, Limnephilidae) in Griechenland. Annales Musei Goulandris 9:457–470. Google Scholar

  40. H. Malicky 2004. Atlas of European Trichoptera. 2nd edition. Springer. Dodrecht, The Netherlands. Google Scholar

  41. H. Malicky 2005. Ein kommentiertes Verzeichnis der Köcherfliegen (Trichoptera) Europas und des Mediterrangebietes. Linzer biologische Beiträge 37:533–596. Google Scholar

  42. H. Malicky 2006. Mitteleuropäische (extra-mediterrane) Arealkerne des Dinodal am Beispiel von Köcherfliegen (Trichoptera). Beiträge zur Entomologie 56:347–359. Google Scholar

  43. H. Malicky and C. Krušnik . 1991. Beobachtungen über die Lebensweise und Beschreibung von Potamophylax winneguthi (Klapálek, 1902) (Trichoptera, Limnephilidae). Zeitschrift der Arbeitsgemeinschaft Österreichischer Entomologen 43:111–116. Google Scholar

  44. H. Malicky, C. Krušnik, G. Moretti, and S. Nógradi . 1986. Ein Beitrag zur Kenntnis der Chaetopteryx rugulosa Kolenati, 1848, Gruppe (Trichoptera, Limnephilidae). Entomofauna – Zeitschrift für Entomologie 7:1–27. Google Scholar

  45. F. Manni, E. Guérard, and E. Heyer . 2004. Geographic patterns of (genetic, morphologic, linguistic) variation: how barriers can be detected by “Monmonier's algorithm”. Human Biology 76:173–190. Google Scholar

  46. P. Mardulyn 2001. Phylogeography of the Vosges mountains populations of Gonioctena pallida (Coleoptera: Chrysomelidae): a nested clade analysis of mitochondrial DNA haplotypes. Molecular Ecology 10:1751–1763. Google Scholar

  47. G. S. Medvedev 1998. Keys to the insects of the European Part of the USSR. Volume IV. Part VI (Megaloptera, Raphidioptera, Neuroptera, Mecoptera, Trichoptera). Akademii Nauk SSSR, Zoologicheskii Institut. Science Publishers, Inc. Enfield, New Hampshire. Google Scholar

  48. W. Mey 1991. Faunistische Daten über Köcherfliegen der Ostpaläarktis und Beschreibung neuer Arten (Insecta, Trichoptera). Deutsche Entomologische Zeitschrift 38:349–363. Google Scholar

  49. W. Mey and L. Botosaneanu . 1985. Glazial-refugiale Subspeziation von Psilopteryx psorosa s.l. (Kolenati, 1860) in den Karpaten und angrenzenden Mittelgebirgen Zentraleuropas (Trichoptera, Limnephilidae). Deutsche Entomologische Zeitschrift 32:109–127. Google Scholar

  50. M. Monmonier 1973. Maximum-difference barriers: an alternative numerical regionalization method. Geographical Analysis 3:245–261. Google Scholar

  51. P. Mráz, M. Gaudeul, D. Rioux, L. Gielly, P. Choler, and P. Taberlet . the IntraBioDiv Consortium 2007. Genetic structure of Hypochaeris uniflora (Asteraceae) suggests vicariance in the Carpathians and rapid post-glacial colonization of the Alps from an eastern Alpine refugium. Journal of Biogeography 34:2100–2114. Google Scholar

  52. C. Muster and T. U. Berendonk . 2006. Divergence and diversity: lessons from arctic-alpine distribution (Pardosa saltuaria group, Lycosidae). Molecular Ecology 15:2921–2933. Google Scholar

  53. Y. Naciri and M. Gaudeul . 2007. Phylogeography of the endangered Eryngium alpinum L. (Apiaceae) in the European Alps. Molecular Ecology 16:2721–2733. Google Scholar

  54. S. R. Narum 2006. Beyond Bonferroni: less conservative analyses for conservation genetics. Conservation Genetics 7:783–787. Google Scholar

  55. S. U. Pauls, H. T. Lumbsch, and P. Haase . 2006. Phylogeography of the montane caddisfly Drusus discolor: evidence for multiple refugia and periglacial survival. Molecular Ecology 15:2153–2169. Google Scholar

  56. S. U. Pauls, K. Theissinger, L. Ujvárosi, M. Bálint, and P. Haase . 2009. Patterns of population structure in two closely related, partially sympatric caddisflies in Eastern Europe: historic introgression, limited dispersal, and cryptic diversity. Journal of the North American Benthological Society 28:517–536. Google Scholar

  57. I. Petersen, Z. Masters, A. G. Hildrew, and S. J. Ormerod . 2004. Dispersal of adult aquatic insects in catchments of differing land use. Journal of Applied Ecology 41:934–950. Google Scholar

  58. S. J. Phillips, R. P. Anderson, and R. E. Schapire . 2006. Maximum entropy modeling of species geographic distributions. Ecological Modelling 190:231–259. Google Scholar

  59. T. Pitsch 1993. Zur Larvaltaxonomie, Faunistik und Ökologie mitteleuropäischer Fließwasser-Köcherfliegen (Insecta: Trichoptera). Universitätsverlag der Technischen Universität Berlin. Berlin, Germany. Google Scholar

  60. D. Posada and K. A. Crandall . 1998. Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818. Google Scholar

  61. A. Previšić, C. Walton, M. Kucinić, P. T. Mitrikeski, and M. Kerovec . 2009. Pleistocene divergence of Dinaric Drusus endemics (Trichoptera, Limnephilidae) in multiple microrefugia within the Balkan Peninsula. Molecular Ecology 18:634–647. Google Scholar

  62. M. Raymond and F. Rousset . 1995. An exact test for population differentiation. Evolution 49:1280–1283. Google Scholar

  63. C. L. Richards, B. C. Carstens, and L. L. Knowles . 2007. Distribution modeling and statistical phylogeography: an integrative framework for generating and testing alternative biogeographical hypotheses. Journal of Biogeography 34:1833–1845. Google Scholar

  64. M. Ronikier, E. Cieślak, and G. Korbecka . 2008. High genetic differentiation in the alpine plant Campanula alpine Jacq. (Campanulaceae): evidence for glacial survival in several Carpathian regions and long-term isolation between the Carpathians and the Alps. Molecular Ecology 17:1763–1775. Google Scholar

  65. F. Ronquist and J. P. Huelsenbeck . 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. Google Scholar

  66. H. H. Ross and W. E. Ricker . 1971. The classification, evolution, and dispersal of the winter stonefly genus Allocapnia. Illinois Biological Monographs 45:1–166. Google Scholar

  67. J. Rozas, J. C. Sánchez-DelBarrio, X. Messeguer, and R. Rozas . 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19:2496–2497. Google Scholar

  68. T. Schmitt 2007. Molecular biogeography of Europe: Pleistocene cycles and postglacial trends. Frontiers in Zoology 4:11. Google Scholar

  69. T. Schmitt and K. Haubrich . 2008. The genetic structure of the mountain forest butterfly Erebia euryale unravels the late Pleistocene and postglacial history of the mountain coniferous forest biome in Europe. Molecular Ecology 17:2194–2207. Google Scholar

  70. T. Schmitt, G. M. Hewitt, and P. Müller . 2006. Disjunct distributions during glacial and interglacial periods in mountain butterflies: Erebia epiphron as an example. Journal of Evolutionary Biology 19:108–113. Google Scholar

  71. R. S. Sommer and N. Benecke . 2005. The recolonization of Europe by brown bears Ursus arctos Linnaeus, 1758 after the Last Glacial Maximum. Mammal Review 35:156–164. Google Scholar

  72. I. Stehlik, F. Blattner, R. Holderegger, and K. Bachmann . 2002. Nunatak survival of the high Alpine plant Eritrichium nanum (L.) Gaudin in the central Alps during the ice ages. Molecular Ecology 11:2027–2036. Google Scholar

  73. J. R. Stewart, A. Lister, I. Barnes, and L. Dalén . 2009. Refugia revisited: individualistic responses of species in space and time. Proceedings of the Royal Society of London Series B: Biological Sciences 277:661–671. Google Scholar

  74. F. Tajima 1993a. Measurement of DNA polymorphism. 37–59. in N. Takahata and A. G. Clark . (editors). Mechanisms of molecular evolution. Introduction to molecular paleopopulation biology. Japan Scientific Societies Press, Sinauer Associates. Sunderland, Massachusetts. Google Scholar

  75. F. Tajima 1993b. Simple methods for testing molecular clock hypothesis. Genetics 135:599–607. Google Scholar

  76. F. Tajima 1996. The amount of DNA polymorphism maintained in a finite population when the neutral mutation rate varies among sites. Genetics 143:1457–1465. Google Scholar

  77. M. M. Tollefsrud, R. Kissling, F. Gugerli, O. Johnsen, T. Skrøppa, R. Cheddadi, W. O. van der Knaap, M. Latałowa, R. Terhürne-Berson, T. Litt, T. Geburek, C. Brochmann, and C. Sperisen . 2008. Genetic consequences of glacial survival and postglacial colonization in Norway spruce: combined analysis of mitochondrial DNA and fossil pollen. Molecular Ecology 17:4134–4150. Google Scholar

  78. S. Ursenbacher, M. Carlsson, V. Helfer, H. Tegelström, and L. Fumagalli . 2006. Phylogeography and Pleistocene refugia of the adder (Vipera berus) as inferred from mitochondrial DNA sequence data. Molecular Ecology 15:3425–3437. Google Scholar

  79. F. A. M. Volckaert, B. Hänfling, B. Hellemans, and G. R. Carvalho . 2002. Timing of the population dynamics of bullhead Cottus gobio (Teleostei: Cottidae) during the Pleistocene. Journal of Evolutionary Biology 15:930–944. Google Scholar

  80. R. Wagner 1991. Life cycles of some autumn emerging caddisflies. 171–175. in C. Tomszewski (editor). Proceedings of the 6th International Symposium on Trichoptera. Adam Mickiewicz University Press. Poznan, Poland. Google Scholar

  81. J. Waringer and W. Graf . 1997. Atlas der österreichischen Köcherfliegenlarven unter Einschluss der angrenzenden Gebiete. Facultas Universitätsverlag. Vienna, Austria. Google Scholar

  82. J. Waringer, W. Graf, S. U. Pauls, and V. Lubini . 2007. The larva of Drusus nigrescens Meyer-Dür, 1875 (Trichoptera: Limnephilidae: Drusinae) with notes on its ecology, genetic differentiation and systematic position. Annales de Limnologie — International Journal of Limnology 43:161–166. Google Scholar

  83. E. Willassen 1996. A nival Bryophaenocladius Thienemann, 1934, with reduced wings (Insecta: Diptera: Chironomidae) (Insecta: Diptera: Chironomidae). Annalen des Naturhistorischen Museums in Wien 98B:507–512. Google Scholar

  84. D-X. Zhang and G. M. Hewitt . 1996. Assessment of the universality and utility of a set of conserved mitochondrial COI primers in insects. Insect Molecular Biology 6:143–150. Google Scholar


Appendix 1. Climate Niche Modeling

We estimated climate niche models with the maximum entropy method implemented in Maxent (Phillips et al. 2006). All climatic data (19 bioclimatic variables) with a resolution of 5 km2 (2.5 arc min) were obtained from the WorldClim global climatic database (Hijmans et al. 2005; available from: We generated climate niche models for the last glacial maximum (LGM) under 2 scenarios, a Community Climate System Model (CCSM) and a Model for Interdisciplinary Research on Climate (MIROC), using default settings in Maxent. We based models on 43 sites from our own sampling (Appendix 2) or from literature data (see Table A1 below). The models were cross-validated in 10 parallel runs. We created binary distribution maps with equal test sensitivity and specificity logistic thresholds. Both models predicted a contiguous potential LGM range across much of central and northwestern Europe, and isolated refugia in the Pyrenees. However, the CCSM predicted a break in the potential LGM range near the present-day Adriatic Sea and the Northern Dinaric Alps. The MIROC also predicted a break, but much further east associated with the present-day extent of the Black Sea.

Table A1

Additional observations used for species distribution modeling. Geographic coordinates are given in decimal degrees.


Appendix 2

Chaetopterygopsis maclachlani material used in our study. Country codes are based on International Organization for Standardization (ISO) 3166 codes. Geographic coordinates are given in decimal degrees. N  =  number of individuals in each collection; * denotes number of adults. See Fig. 2 for region abbreviations. Regions are arranged from west to east. Lat  =  latitude, Long  =  longitude, Alt  =  altitude.

Stephanie Lehrian, Miklós Bálint, Peter Haase, and Steffen U. Pauls "Genetic population structure of an autumn-emerging caddisfly with inherently low dispersal capacity and insights into its phylogeography," Journal of the North American Benthological Society 29(3), 1100-1118, (20 July 2010).
Received: 31 July 2009; Accepted: 1 April 2010; Published: 20 July 2010

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