Green Anoles (Anolis carolinensis) are comprised of red-dewlapped (RD) forms that are found throughout the southeastern USA and a gray-dewlapped (GD) form that is restricted to southwest Florida. Prior research has shown that RD A. carolinensis produce headbob displays of three distinct types that differ primarily in their temporal patterns. Based on known morphological, physiological, and genetic differences between GD and RD populations, we hypothesized that these populations also would differ in headbob display structure. To test this hypothesis we quantified 440 displays from 39 males (24 GD and 15 RD) and assigned displays to type using numerical decision criteria. Although comparison of the same display types between GD and RD males revealed differences in the durations of several homologous display units (i.e., bobs or interbob pauses), only one unit differed following statistical correction for multiple comparisons. By taking into account all display variation in both populations simultaneously, however, discriminant function analysis correctly assigned display units with high accuracy to population and display type. Nevertheless, differences in unit durations often were greater between two RD populations occurring within Florida than they were between our GD and RD study populations. Thus, despite our demonstration of differences in the display temporal structure between GD and RD forms of A. carolinensis, these differences appear to be no greater in magnitude than those observed between RD populations.