We describe two new species of Pristimantis from southwestern Colombia. The first new species inhabits the humid Pacific slopes of the Cordillera Occidental, Valle del Cauca; it is most similar to three allopatric Ecuadorian species—P. eugeniae (Lynch and Duellman 1997), P. nyctophylax (Lynch 1976), and P. subsigillatus (Boulenger 1902). It is distinguished, however, by having subarticular tubercles beneath the joint between distal phalanges, basal webbing on outer edge of toe IV, papilla at the tip of snout, and uniform cream-brown coloration on the anterior surfaces of thighs and in the groin. The second new species inhabits the high mountains of the Cordillera Central, Cauca, and it is most similar to P. boulengeri (Lynch 1981) and two Peruvian species—P. deyiLehr et al. 2013 and P. schultei (Duellman 1990). However, it differs by having subarticular tubercles beneath the joint between distal phalanges, dorsal skin with low warts, papilla at the tip of snout, and by lacking tympanic membranes, dentigerous processes of the vomer, and vocal slits.