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1 December 2016 A Fast and Inexpensive High-Throughput Protocol for Isolating High Molecular Weight Genomic DNA from Lichens
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Abstract

Werth, S., Reynisdóttir, S., Guðmundsson, H. & Andrésson, Ó. S. 2016. A fast and inexpensive high-throughput protocol for isolating high molecular weight genomic DNA from lichens. — Herzogia 29: 610–616.

Isolating high molecular weight DNA as required for genomic library preparations and other applications is a challenging task. We optimized a glass-fiber column-based, high throughput DNA isolation protocol to yield comparatively large quantities of high molecular weight, pure DNA; cell lysis based on a CTAB buffer was followed by centrifugation to remove cell debris, binding to a glass-fiber membrane under high concentration of a chaotropic salt (guanidine hydrochloride), followed by two 70 % ethanol washes and elution. This method was utilized successfully on several species of lichenized ascomycetes and on two non-lichenized basidiomycetes. Thus, while developed for lichens, the method is suitable for a range of fungal taxa.

Silke Werth, Sigrún Reynisdóttir, Hörður Gudmundsson, and Ólafur S. Andrésson "A Fast and Inexpensive High-Throughput Protocol for Isolating High Molecular Weight Genomic DNA from Lichens," Herzogia 29(2), 610-616, (1 December 2016). https://doi.org/10.13158/heia.29.2.2016.610
Accepted: 1 June 2016; Published: 1 December 2016
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