Translator Disclaimer
1 April 2001 TRANSFORMING GROWTH FACTOR BETA MAY ACT AS AN AUTOCRINE-SURVIVAL–PROMOTING FACTOR FOR TRANSFORMED TROPHOBLASTS
CHI-KUAN HO, LO-CHUN AU, HO-JEN PENG, SHENG-YUAN WANG
Author Affiliations +
Abstract

 Using five trophoblast cell lines of different differentiation status, we have shown that trophoblasts could constitutively release the transforming growth factor beta-1 (TGFβ1), but not TGFβ2. Treatment of the human tumorigenic, TL, and BeWo cells with a differentiating agent and a potent protein kinase C activator—the tumor-promoting agent—or the JEG-3 cells with cholera toxin—a potent cyclic adenosine 3′:5′monophosphate (cAMP) inducer—or its analogue 8-bromo-cAMP, potentiates TGFβ production, but the two signaling pathways appear to be mutually exclusive. Surprisingly, the JAR cell line failed to respond to either type of TGFβ activator. Based on reverse transcriptase (RT)-polymerase chain reaction (PCR), it was found that only the JAR cell line expressed messenger ribonucleic acid for decorin, a natural inhibitor of TGFβ, and none of the cell lines had detectable protein expression as determined by immunocytochemical studies. The cell number in cultures with decorin was invariably lesser than in those without decorin under serum-free conditions for all the cell lines tested. These results suggest that TGFβ may act as an autocrine-survival factor for transformed trophoblasts by allowing the cells to survive longer under a microenvironment which is not favorable for growth. Lastly, our results indicate that decorin, acting in a paracrine manner, may also play an important negative regulatory role in the development of transformed trophoblasts by sequestering TGFβ, thereby preventing its action.

CHI-KUAN HO, LO-CHUN AU, HO-JEN PENG, and SHENG-YUAN WANG "TRANSFORMING GROWTH FACTOR BETA MAY ACT AS AN AUTOCRINE-SURVIVAL–PROMOTING FACTOR FOR TRANSFORMED TROPHOBLASTS," In Vitro Cellular & Developmental Biology - Animal 37(4), 245-250, (1 April 2001). https://doi.org/10.1290/1071-2690(2001)037<0245:TGFBMA>2.0.CO;2
Received: 23 October 1999; Accepted: 1 November 2000; Published: 1 April 2001
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

Share
SHARE
KEYWORDS
decorin
TGF-β
trophoblasts
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top