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1 September 2002 COLONY-FORMING CAPACITY OF PORCINE LIVER EPITHELIAL CELLS IN CULTURE
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Abstract

Previously, we reported the presence of certain nonparenchymal epithelial cells (NPECs) in adult porcine livers that demonstrate differentiation patterns including an emergence of duct-like structures (DLSs) in the colonies. In the present study, we examined the effect of supplements to the NAIR-1 medium (Dulbecco modified Eagle medium [DMEM]–F12 containing 5% fetal bovine serum [FBS] and 11 supplements) used in these cultures on formation of DLSs-emerged colonies (type I colonies). No type I colonies were observed in the cultures of the nonparenchymal cell fraction when Roswell Park Memorial Institute–1640 medium or DMEM–F12 (1:1) supplemented with 5% FBS was used as the culture medium. NAIR-1 medium without each component did not produce any significant results. No type I colonies were formed when epidermal growth factor, and hydrocortisone and insulin mixture (A) or nicotinamide and l-ascorbic acid phosphate magnesium salt (Asc2P) mixture (B) was added to the DMEM–F12 medium supplemented with 5% FBS. However, when a combination of A and B was added, colonies were formed at a significant level. Together, the number of type I colonies was increased in the combination of A and B containing a higher concentration of Asc2P. We conclude that NPECs need a mixture of Asc2P and other components as supplements for type 1 colony formation.

TAKAYOSHI TOKIWA, TAISUKE YAMAZAKI, JUNKO KANO, MASAYUKI NOGUCHI, SHIN ENOSAWA, and MINORU KOHNO "COLONY-FORMING CAPACITY OF PORCINE LIVER EPITHELIAL CELLS IN CULTURE," In Vitro Cellular & Developmental Biology - Animal 38(8), 440-442, (1 September 2002). https://doi.org/10.1290/1071-2690(2002)038<0440:CFCOPL>2.0.CO;2
Received: 24 October 2002; Accepted: 1 November 2002; Published: 1 September 2002
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