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1 January 2004 ACTION OF MAJOR INSECTICIDE GROUPS ON INSECT CELL LINES OF THE BEET ARMYWORM, SPODOPTERA EXIGUA, COMPARED WITH LARVICIDAL TOXICITY
LUC DECOMBEL, GUY SMAGGHE, LUC TIRRY
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Abstract

In a first series of experiments, the biological response of a continuous cell line of the beet armyworm, Spodoptera exigua, was tested with different groups of insecticides with different modes of actions: acetylcholinesterase inhibitors, acetycholine receptor agonists, inhibitors and uncouplers of oxidative phosphorylation, site I electron transport inhibitors, gamma-aminobutyric acid receptor inhibitors, chitin synthesis inhibitors, and juvenile hormone analogues. From the concentration response curves, 50% inhibition concentration (IC50) values were calculated. The most active compound in vitro was pyridaben with an IC50 value of 0.0083 ppm. In a second series of experiments, the toxicity of these insecticide groups was determined on third-instar larvae of S. exigua, and lethal concentration with 50% kill (LC50) values were used in the evaluation of their in vivo biological activity. Toxicity bioassays showed that lufenuron was the most toxic (LC50 = 0.098 ppm). To explain the discrepancies in biological responses in vitro with insect cells compared with in vivo conditions with whole third-instar larvae, the significance of different detoxifying enzyme systems was tested. P450 monooxygenases, esterases, and glutathione S-transferases were measured in third-instar larvae and cells of S. exigua. Data are discussed in terms of the usefulness of insect cell cultures as tools in the screening for novel insecticide actions.

LUC DECOMBEL, GUY SMAGGHE, and LUC TIRRY "ACTION OF MAJOR INSECTICIDE GROUPS ON INSECT CELL LINES OF THE BEET ARMYWORM, SPODOPTERA EXIGUA, COMPARED WITH LARVICIDAL TOXICITY," In Vitro Cellular & Developmental Biology - Animal 40(1), 43-51, (1 January 2004). https://doi.org/10.1290/1543-706X(2004)40<43:AOMIGO>2.0.CO;2
Received: 27 August 2003; Accepted: 29 January 2004; Published: 1 January 2004
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