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1 November 2004 INTERLEUKIN-8 CAN STIMULATE PROGESTERONE SECRETION FROM A HUMAN TROPHOBLAST CELL LINE, BEWO
KUAN-HAO TSUI, LING-YUN CHEN, MIAWH-LIRNG SHIEH, SHENG-PING CHANG, CHIOU-CHUNG YUAN, HSIN-YANG LI
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Abstract

Precise paracrine cross-talk between the embryo and the endometrium is essential for the establishment of a successful pregnancy. Previous studies have demonstrated that the expression of interleukin-8 (IL-8) in the endometrium is enhanced during the late-secretory phase and early pregnancy. Furthermore, IL-8 receptor (IL-8R) expression has been detected in trophoblast cells of the developing embryo. To clarify the roles of IL-8 in the endometrium–embryo interactions, the effects of IL-8 on hormone secretion by trophoblast cells were studied using the BeWo trophoblast cell line that retains hormone-secreting properties of normal trophoblast cells. Using reverse transcription–polymerase chain reaction, we found that IL-8R messenger ribonucleic acid (mRNA) was expressed in BeWo cells. The levels of IL-8R mRNA and protein expression in BeWo cells were similar to those in primary first-trimester trophoblast cells. Progesterone (P4) secretion of BeWo cells was comparable with that of first-trimester trophoblast cells but higher than that of third-trimester trophoblast cells. Treatment of BeWo cells with recombinant human IL-8 (rhIL-8) had no effect on cell proliferation, as determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Interestingly, secretion of P4, but not human chorionic gonadotropin, from cultured BeWo cells was significantly enhanced when the cells were incubated with rhIL-8. Our results demonstrate that IL-8 may play an important role in the endometrium–embryo interactions by stimulating trophoblast secretion of P4 for maintenance of a successful pregnancy.

KUAN-HAO TSUI, LING-YUN CHEN, MIAWH-LIRNG SHIEH, SHENG-PING CHANG, CHIOU-CHUNG YUAN, and HSIN-YANG LI "INTERLEUKIN-8 CAN STIMULATE PROGESTERONE SECRETION FROM A HUMAN TROPHOBLAST CELL LINE, BEWO," In Vitro Cellular & Developmental Biology - Animal 40(10), 331-336, (1 November 2004). https://doi.org/10.1290/0404033.1
Received: 12 April 2004; Accepted: 1 December 2004; Published: 1 November 2004
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KEYWORDS
embryo implantation
hCG
IL-8
P4
Trophectoderm
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