Translator Disclaimer
1 March 2004 DEVELOPMENT AND PARTIAL CHARACTERIZATION OF HELIOTHINE CELL LINES FROM EMBRYONIC AND DIFFERENTIATED TISSUES
CYNTHIA L. GOODMAN, AMY A. WANG, HENDA NABLI, ARTHUR H. MCINTOSH, JENNIFER L. WITTMEYER, JAMES J. GRASELA
Author Affiliations +
Abstract

The goal of this study was to generate cell lines from a variety of insect tissues that could be useful for developing in vitro assays with tissue-specific properties. In this article, we describe the establishment of new cell cultures from differentiated (primarily neural) and undifferentiated tissues (primarily embryonic) and their initial characterization. Cell lines were established from the following tissues of the budworm, Heliothis virescens, and the bollworm, Helicoverpa zea: larval ventral nerve cords (4 lines), larval midguts (1 line), adult ovaries (1 line), and embryonic tissues (11 lines). Cell lines were primarily characterized by morphological examination and polymerase chain reaction (PCR) (both deoxyribonucleic acid amplification fingerprinting and inter–simple sequence repeats PCR).

CYNTHIA L. GOODMAN, AMY A. WANG, HENDA NABLI, ARTHUR H. MCINTOSH, JENNIFER L. WITTMEYER, and JAMES J. GRASELA "DEVELOPMENT AND PARTIAL CHARACTERIZATION OF HELIOTHINE CELL LINES FROM EMBRYONIC AND DIFFERENTIATED TISSUES," In Vitro Cellular & Developmental Biology - Animal 40(3), 89-94, (1 March 2004). https://doi.org/10.1290/1543-706X(2004)040<0089:DAPCOH>2.0.CO;2
Received: 14 August 2003; Accepted: 25 March 2004; Published: 1 March 2004
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top