Translator Disclaimer
1 November 2005 ADIPOSE DIFFERENTIATION RELATED PROTEIN INDUCES LIPID ACCUMULATION AND LIPID DROPLET FORMATION IN HEPATIC STELLATE CELLS
MARIE FUKUSHIMA, MUNECHIKA ENJOJI, MOTOYUKI KOHJIMA, RIE SUGIMOTO, SATOSHI OHTA, KAZUHIRO KOTOH, MASAMI KUNIYOSHI, KUNIHISA KOBAYASHI, MINAKO IMAMURA, TOYOSHI INOGUCHI, MAKOTO NAKAMUTA, HAJIME NAWATA
Author Affiliations +
Abstract

The function of adipose differentiation–related protein (ADRP) is known to be the uptake of long-chain fatty acids and formation of lipid droplets in lipid-accumulating cells. We hypothesized that ADRP might stimulate activated hepatic stellate cells (HSCs) to accumulate lipids, resulting in their transition to the quiescent state. In this study, cultured HSCs in fifth passages isolated from rat were infected by adenovirus vector expressing ADRP (Ad.GFP-ADRP), and morphologic and functional changes were evaluated in comparison with control HSCs infected by recombinant adenovirus-expressing β-galactosidase (Ad.LacZ). In Ad.GFP-ADRP–infected cells only, many tiny lipid droplets were apparent in the cytoplasm, while the outline of the cells was not changed. The ADRP was detected around the lipid droplets. In HSCs with intracellular actin filaments, the staining pattern of the filaments before and after infection with Ad.GFP-ADRP or Ad.LacZ did not differ. The cell proliferation rate was not influenced by infection with Ad.LacZ or Ad.GFP-ADRP. Type I collagen secretion from cells overexpressing ADRP was not significantly different from that of Ad.LacZ-infected cells. In our in vitro study, ADRP overexpression induced the formation of cytoplasmic lipid droplets in activated HSCs but could not convert other characteristics of the activated form into those of the quiescent form.

MARIE FUKUSHIMA, MUNECHIKA ENJOJI, MOTOYUKI KOHJIMA, RIE SUGIMOTO, SATOSHI OHTA, KAZUHIRO KOTOH, MASAMI KUNIYOSHI, KUNIHISA KOBAYASHI, MINAKO IMAMURA, TOYOSHI INOGUCHI, MAKOTO NAKAMUTA, and HAJIME NAWATA "ADIPOSE DIFFERENTIATION RELATED PROTEIN INDUCES LIPID ACCUMULATION AND LIPID DROPLET FORMATION IN HEPATIC STELLATE CELLS," In Vitro Cellular & Developmental Biology - Animal 41(10), 321-324, (1 November 2005). https://doi.org/10.1290/0410069.1
Received: 19 December 2004; Accepted: 1 March 2005; Published: 1 November 2005
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

Share
SHARE
KEYWORDS
ADRP
Collagen
fatty acid
stellate cells
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top