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1 November 2005 ADULT RAT SPINAL CORD CULTURE ON AN ORGANOSILANE SURFACE IN A NOVEL SERUM-FREE MEDIUM
MAINAK DAS, NEELIMA BHARGAVA, CASSIE GREGORY, LISA RIEDEL, PETER MOLNAR, JAMES J. HICKMAN
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Abstract

In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology, the development of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth–promoting organosilane substrate coated on a glass surface and an empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3, brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the cultures were maintained for 4–6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional in vitro system.

MAINAK DAS, NEELIMA BHARGAVA, CASSIE GREGORY, LISA RIEDEL, PETER MOLNAR, and JAMES J. HICKMAN "ADULT RAT SPINAL CORD CULTURE ON AN ORGANOSILANE SURFACE IN A NOVEL SERUM-FREE MEDIUM," In Vitro Cellular & Developmental Biology - Animal 41(10), 343-348, (1 November 2005). https://doi.org/10.1290/0505031.1
Received: 9 May 2005; Accepted: 1 June 2005; Published: 1 November 2005
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KEYWORDS
adult rat spinal cord
Cell culture
electrophysiology
motoneuron
Serum-free medium
silane surface
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