Translator Disclaimer
1 March 2005 EFFECT OF DEXAMETHASONE ON BINDING ACTIVITY OF TRANSCRIPTION FACTORS NUCLEAR FACTOR-κB AND ACTIVATOR PROTEIN-1 IN SW982 HUMAN SYNOVIAL SARCOMA CELLS
TAISUKE YAMAZAKI, TAKASHI TUKIYAMA, TAKAYOSHI TOKIWA
Author Affiliations +
Abstract

Previously, we showed that dexamethasone (DEX) inhibited the expression of inflammatory cytokines, matrix metalloproteinase-1, and cyclooxygenase-2 messenger ribonucleic acid in SW982 cells. In this study, the effect of DEX on the transcription factors nuclear factor–κB (NF-κB) and activator protein–1 (AP-1) was examined in SW982 cells by electrophoretic mobility shift assay (EMSA). Both NF-κB and AP-1 deoxyribonucleic acid binding activities were detectable in SW982 cells by EMSA, and they were induced by interleukin-1β treatment. DEX inhibited NF-κB binding activity at 10 μM as well as at 100 μM, although the inhibition was only partial. However, DEX had little effect on AP-1 activity. These results suggest that DEX reduces the expression of inflammatory cytokines and other proteins in SW982 cells by inhibiting NF-κB.

TAISUKE YAMAZAKI, TAKASHI TUKIYAMA, and TAKAYOSHI TOKIWA "EFFECT OF DEXAMETHASONE ON BINDING ACTIVITY OF TRANSCRIPTION FACTORS NUCLEAR FACTOR-κB AND ACTIVATOR PROTEIN-1 IN SW982 HUMAN SYNOVIAL SARCOMA CELLS," In Vitro Cellular & Developmental Biology - Animal 41(3), 80-82, (1 March 2005). https://doi.org/10.1290/0502011.1
Received: 11 February 2005; Accepted: 8 March 2005; Published: 1 March 2005
JOURNAL ARTICLE
3 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top