A NEW CELL LINE FROM THE WAX MOTH GALLERIA MELLONELLA LINNE (LEPIDOPTERA: PYRALIDIDAE)

DAISUKE EGUCHI; KIKUO IWABUCHI

doi:10.1290/0509057.1

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DAISUKE EGUCHI,¹ KIKUO IWABUCHI^1,*

¹Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, Tokyo 183-8509 Japan

^*To whom correspondence should be addressed at Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, Tokyo 183-8509 Japan. kikkuo@cc.tuat.ac.jp

Abstract

A cell line derived from the larval-fat body tissues of the wax moth, Galleria mellonella Linne, was established in MGM-450 medium. The cells grew in suspension and were mainly spherical in shape. Population doubling time was between 1.4 and 1.7 d over a range of 15 to 35° C, and the maximum growth rate was at 25° C. The chromosome number ranged from 70–239, with a mode of 170. The cells were sensitive to 20-hydroxyecdysone, which stimulated their growth and induced morphological changes. The cell line was designated GaMe-LF1.

Citation Download Citation

DAISUKE EGUCHI and KIKUO IWABUCHI "A NEW CELL LINE FROM THE WAX MOTH GALLERIA MELLONELLA LINNE (LEPIDOPTERA: PYRALIDIDAE)," In Vitro Cellular & Developmental Biology - Animal 42(1), 1-3, (1 January 2006). https://doi.org/10.1290/0509057.1

Received: 13 September 2005; Accepted: 30 November 2005; Published: 1 January 2006