An improved assay to quantitate the invasiveness of cells in modified Boyden chambers

Michelle M. M. Woo; Clara M. Salamanca; Agata Minor; Nelly Auersperg

doi:10.1007/s11626-006-9002-4

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Michelle M. M. Woo,^1,2 Clara M. Salamanca,^1,2 Agata Minor,^1,2 Nelly Auersperg³

¹Child and Family Research Centre, University of British Columbia, Vancouver, BC, Canada
²Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, BC, Canada
³Department of Obstetrics and Gynecology, University of British Columbia, B.C. Women's Hospital, 4490 Oak Street, Vancouver, BC V6H 3V5, Canada, e-mail: auersper@interchange.ubc.ca

Abstract

The most commonly used means of assessing the invasiveness of cultured cells is the Boyden chamber assay, which requires that cells lyse Matrigel™, followed by migration through pores in a filter in response to a chemotactic gradient. This report describes a simple method, which greatly increases the speed and accuracy by which Boyden chamber assays can be analyzed, and permits the concurrent analysis of distinct cell subpopulations within specimens containing multiple-cell types.

Citation Download Citation

Michelle M. M. Woo, Clara M. Salamanca, Agata Minor, and Nelly Auersperg "An improved assay to quantitate the invasiveness of cells in modified Boyden chambers," In Vitro Cellular & Developmental Biology - Animal 43(1), 7-9, (13 February 2007). https://doi.org/10.1007/s11626-006-9002-4

Received: 11 August 2006; Accepted: 25 October 2006; Published: 13 February 2007