Translator Disclaimer
24 January 2008 A novel immortalization vector for the establishment of penaeid shrimp cell lines
Guo-bin Hu, Dan Wang, Chang-hong Wang, Kun-feng Yang
Author Affiliations +
Abstract

Cell immortalization technology based on gene transfer has been successfully used to generate cell lines from a wide variety of cell types. The inability to stably introduce and express foreign genes has hampered application of this strategy in shrimp cells. We report here the use of replication-defective pantropic retrovirus to achieve a novel immortalization vector in which simian virus 40 large T antigen (SV40T) gene is expressed from Moloney murine leukemia virus (MoMLV) promoter. Data confirmed the presence of transferred SV40T gene and its stable mRNA expression in transduced lymphoid cells of Penaeus chinensis. The transduced cells showed a higher growth rate and a longer replication life-span compared with their untransduced counterparts. These results indicate the pantropic retrovirus-based immortalization-inducing gene delivery system is a potential tool for establishing cell lines from shrimp.

Guo-bin Hu, Dan Wang, Chang-hong Wang, and Kun-feng Yang "A novel immortalization vector for the establishment of penaeid shrimp cell lines," In Vitro Cellular & Developmental Biology - Animal 44(3), 51-56, (24 January 2008). https://doi.org/10.1007/s11626-007-9076-7
Received: 6 August 2007; Accepted: 27 November 2007; Published: 24 January 2008
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top