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14 June 2008 Rapid identification and authentication of closely related animal cell culture by polymerase chain reaction
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Abstract

Animal cell lines are important resources for research and diagnostic applications. Cross-contamination and misidentification of cell lines, however, can cause major problems for research (for example, false results that come from contamination cells may mislead the science). Hence, it is imperative to routinely monitor cell lines for identity and authenticity. Here, we extend our previous work on identification and authentication of animal cell culture by polymerase chain reaction (PCR) amplification and DNA sequencing. A PCR-based method for rapid identification and authentication of closely related cell lines was described. In this method, two new primers were designed based on high homology in the aldolase gene family. Used together with our previous primers, the combinations of primers were able to differentiate closely related species, including human from monkey and mouse from rat. This PCR assay provides a rapid, simple, sensitive, and cost-effective method for authentication of closely related cell lines.

Merry Liu, Hsi Liu, Xiaoling Tang, Abbas Vafai, and J. Denry Sato "Rapid identification and authentication of closely related animal cell culture by polymerase chain reaction," In Vitro Cellular & Developmental Biology - Animal 44(7), 224-227, (14 June 2008). https://doi.org/10.1007/s11626-008-9121-1
Received: 27 February 2008; Accepted: 1 April 2008; Published: 14 June 2008
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