Translator Disclaimer
1 September 2003 ONE HUNDRED YEARS OF ZYGOTIC EMBRYO CULTURE INVESTIGATIONS
V. RAGHAVAN
Author Affiliations +
Abstract

Isolation of zygotic embryos from seeds and their culture in a defined medium, initiated by Hannig in 1904, has proved to be a promising method to study the factors that control growth and differentiation of embryos. Using this technique, several investigations have focused on the carbohydrate and nitrogen nutrition during germination of cultured seed embryos and on the effects of plant hormones on their morphogenesis. Culture of immature embryos leads to their germination into weak seedlings, skipping the later stages of embryogenesis, by a process known as precocious germination. Progressively smaller embryos have been cultured by supplementation of the medium with coconut milk or hormonal additives or by osmotic adjustment of the medium by high concentrations of sucrose or mannitol. Although methods have not been developed for large-scale isolation and culture of zygotes, zygotes of maize isolated from embryo sacs and those obtained by in vitro fertilization have been grown in culture into full-term embryos. Embryo culture techniques are widely used to rescue embryos from seeds of wide crosses which usually abort and to overcome dormancy of recalcitrant seeds.

V. RAGHAVAN "ONE HUNDRED YEARS OF ZYGOTIC EMBRYO CULTURE INVESTIGATIONS," In Vitro Cellular and Developmental Biology - Plant 39(5), 437-442, (1 September 2003). https://doi.org/10.1079/IVP2003436
Received: 5 February 2003; Accepted: 1 February 2003; Published: 1 September 2003
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

Share
SHARE
KEYWORDS
embryo morphogenesis
historical review
hybrid rescue
overcoming seed dormancy
proembryo culture
zygote culture
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top