Translator Disclaimer
1 September 2003 IN VITRO CULTURE STUDIES ON STEVIA REBAUDIANA
LATHA SIVARAM, USHA MUKUNDAN
Author Affiliations +
Abstract

Shoot apex, nodal, and leaf explants of Stevia rebaudiana Bertoni can regenerate shoots when cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA; 8.87 μM) and indole-3-acetic acid (5.71 μM). Rooting of the in vitro-derived shoots could be achieved following subculture onto auxin-containing medium. A survival rate of 70% was recorded at the hardening phase on the substrate cocopeat. The presence of the sweet diterpene glycosides, viz. stevioside and rebaudioside, was confirmed in the in vitro-derived tissues of Stevia using HPTLC techniques. Callus cultured on agar-solidified MS medium supplemented with BA (8.87 μM) and indole-3-butyric acid (9.80 μM) showed the highest sweetener content.

LATHA SIVARAM and USHA MUKUNDAN "IN VITRO CULTURE STUDIES ON STEVIA REBAUDIANA," In Vitro Cellular and Developmental Biology - Plant 39(5), 520-523, (1 September 2003). https://doi.org/10.1079/IVP2003438
Received: 15 February 2002; Accepted: 1 January 2003; Published: 1 September 2003
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top