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1 September 2003 PRODUCTION, STORABILITY, AND REGENERATION OF SHOOT TIPS OF POTATO (SOLANUM TUBEROSUM L.) ENCAPSULATED IN CALCIUM ALGINATE HOLLOW BEADS
AGGREY BERNARD NYENDE, SIEGFRIED SCHITTENHELM, GUNDA MIX-WAGNER, JÖRG-MICHEAL GREEF
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Abstract

Shoot tips, of four potato cultivars (Désirée, Genet, Tigoni, and Tomensa), 3–4 mm in size, were precultured for 2 d on Murashige and Skoog (MS) solid medium, then encapsulated in calcium alginate to produce hollow bead synthetic seed capsules averaging 0.78 cm in diameter. Regeneration and ‘regrowth’ were tested on MS solid medium and on soil in the greenhouse, respectively. The encapsulated shoot tips were stored at 4 and 10°C for up to 390 d. For all cultivars, the encapsulated shoot tips stored at both temperatures for 180 d and at 4°C for 270 d, 100% regeneration on MS solid medium was recorded. After 360 d in storage at 4°C, 70.8% (Tigoni), 66.7% (Genet), 58.3% (Désirée), and 51.5% (Tomensa) regeneration was recorded on MS medium, reducing to 15% (Tigoni), 25% (Genet), 10% (Désirée), and 0% (Tomensa) regeneration after 390 d in storage. ‘Regrowth’ of 93–100% was recorded for non-stored encapsulated shoot tips, directly transferred on soil in the greenhouse after a 2 wk preculture on MS solid medium with an added fungicide (carbendazim) in the encapsulating gel. The ‘regrown’ shoot tips produced plants showing normal development. The results presented here demonstrate that hollow bead synthetic seed capsules are an alternative propagating method for potato seed production.

AGGREY BERNARD NYENDE, SIEGFRIED SCHITTENHELM, GUNDA MIX-WAGNER, and JÖRG-MICHEAL GREEF "PRODUCTION, STORABILITY, AND REGENERATION OF SHOOT TIPS OF POTATO (SOLANUM TUBEROSUM L.) ENCAPSULATED IN CALCIUM ALGINATE HOLLOW BEADS," In Vitro Cellular and Developmental Biology - Plant 39(5), 540-544, (1 September 2003). https://doi.org/10.1079/IVP2003442
Received: 3 October 2002; Accepted: 1 February 2003; Published: 1 September 2003
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KEYWORDS
regeneration
shoot tip encapsulation
Solanum tuberosum L.
synthetic seeds
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