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1 September 2003 DEVISING AN IN VITRO NODULATION SYSTEM FOR TWO ACTINORHIZAL PLANTS: ALLOCASUARINA VERTICILLATA (LAM.) L. JOHNSON AND CASUARINA GLAUCA SIEB. EX SPRENG (CASUARINACEAE)
MAME OUREYE SY, MAURICE SAGNA, SIRE DIEDHIOU, CLAUDINE FRANCHE, FLORENCE AUGUY, EMILE DUHOUX
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Abstract

The purpose of this study was to establish an efficient in vitro nodulation device for producing actinorhizal root nodules on Allocasuarina verticillata and Casuarina glauca. Seeds from the two species were germinated aseptically and seedlings with at least two photosynthetic branchlets and a 3–5 cm long root system were transferred into Petri dishes containing a biphasic (solid/liquid) medium. To assess the nodulation capacity, four different culture media were tested. As soon as the root system developed and spread adequately on the surface of the medium, plants were deprived of nitrogen for at least 1 wk and inoculated with the Frankia strain. The time course nodulation for A. verticillata showed that the basal Hoagland medium supplemented with CaCO3 and KNO3 was most efficient, with 83% of plantlets forming nodules, while the medium supplemented with CaCO3 reached 100% nodulation for C. glauca. This procedure can provide a valuable tool for the study of early events of actinorhizal nodulation and spatio-temporal expression of symbiotic genes in transgenic Casuarinaceae.

MAME OUREYE SY, MAURICE SAGNA, SIRE DIEDHIOU, CLAUDINE FRANCHE, FLORENCE AUGUY, and EMILE DUHOUX "DEVISING AN IN VITRO NODULATION SYSTEM FOR TWO ACTINORHIZAL PLANTS: ALLOCASUARINA VERTICILLATA (LAM.) L. JOHNSON AND CASUARINA GLAUCA SIEB. EX SPRENG (CASUARINACEAE)," In Vitro Cellular and Developmental Biology - Plant 39(5), 545-550, (1 September 2003). https://doi.org/10.1079/IVP2003443
Received: 12 September 2002; Accepted: 1 April 2003; Published: 1 September 2003
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KEYWORDS
actinorhizal nodule
Allocasuarina verticillata
Biological nitrogen fixation
Casuarina glauca
Frankia
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