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1 September 2003 A NEW APPROACH FOR THE BIOLISTIC METHOD: BOMBARDMENT OF LIVING NITROGEN-FIXING BACTERIA INTO PLANT TISSUES
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Abstract

A new utilization of the biolistic gun was developed for the direct introduction of nitrogen-fixing bacteria (Azotobacter vinelandii) into strawberry (Fragaria×ananassa) tissues. This was the first case of using living bacteria as microprojectiles for the bombardment of plant tissues. Bacterial cells, adhered to tungsten particles, were accelerated by a nitrogen-powered device, and delivered into the target leaves and regenerating shoot meristems. The presence of bacteria in the developing strawberry callus tissues and regenerating plants was detected by microscopy, acetylene reduction assay, and selective polymerase chain reaction. Practically, the elaborated method proved to be suitable for the establishment of artificial intercellular associations between nitrogen-fixing bacteria and higher plants.

ÉVA PREININGER, ISTVÁN GYURJÁN, KÁROLY BÓKA, TAMÁS PÓNYI, JÓZSEF ZATYKÓ, and PÁL KORÁNYI "A NEW APPROACH FOR THE BIOLISTIC METHOD: BOMBARDMENT OF LIVING NITROGEN-FIXING BACTERIA INTO PLANT TISSUES," In Vitro Cellular and Developmental Biology - Plant 39(5), 443-449, (1 September 2003). https://doi.org/10.1079/IVP2003444
Received: 25 April 2002; Accepted: 1 February 2003; Published: 1 September 2003
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