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1 September 2003 PLANT REGENERATION BY SOMATIC EMBRYOGENESIS AND ORGANOGENESIS IN COMMERCIAL PINEAPPLE (ANANAS COMOSUS L.)
SUNEERAT SRIPAORAYA, ROBERT MARCHANT, J. BRIAN POWER, MICHAEL R. DAVEY
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Abstract

Axillary and terminal buds from suckers of Ananas comosus cv. Phuket were established on Murashige and Tucker-based (MT) medium with 2.0 mg l−1 (9.8 μM) indolebutyric acid, 2.0 mg l−1 (10.74 μM) naphthaleneacetic acid, and 2.0 mg l−1 (9.29 μM) kinetin, followed by multiplication on Murashige and Skoog-based (MS) medium containing 2.0 mg l−1 (8.87 μM) benzyladenine (BA) to provide a continuous supply of axenic shoots. Leaves, excised from such cultured shoots, produced adventitious shoots from their bases when these explants were cultured on MS medium containing 0.5 mg l−1 (2.26 μM) 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 mg l−1 (8.87 μM) BA. Embryogenic callus was produced when leaf explants were cultured on MS medium with 3.0 mg l−1 (12.42 μM) 4-amino-3,5,6-trichloropicolinic acid (picloram). Somatic embryos developed into shoots following transfer of embryogenic tissues to MS medium with 1.0 mg l−1 (4.44 μM) BA. Cell suspensions, initiated by transfer of embryogenic callus to liquid MS medium with 1.0 mg l−1 (4.14 μM) picloram or 1.0 mg l−1 (4.52 μM) 2,4-D, also regenerated shoots by somatic embryogenesis, on transfer of cells to semisolid MS medium with 1.0 mg l−1 (4.44 μM) BA. All regenerated shoots rooted on growth regulator-free MS medium, prior to ex vitro acclimation and transfer to the glasshouse. These studies provide a baseline for propagation, conservation, and genetic manipulation of elite pineapple germplasms.

SUNEERAT SRIPAORAYA, ROBERT MARCHANT, J. BRIAN POWER, and MICHAEL R. DAVEY "PLANT REGENERATION BY SOMATIC EMBRYOGENESIS AND ORGANOGENESIS IN COMMERCIAL PINEAPPLE (ANANAS COMOSUS L.)," In Vitro Cellular and Developmental Biology - Plant 39(5), 450-454, (1 September 2003). https://doi.org/10.1079/IVP2003445
Received: 19 February 2002; Accepted: 1 January 2003; Published: 1 September 2003
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KEYWORDS
Ananas comosus
Organogenesis
pineapple
Plant regeneration
somatic embryogenesis
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