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1 September 2003 FACTORS INFLUENCING SHOOT REGENERATION FROM COTYLEDONS OF TETRAPLOID ISATIS INDIGOTICA FORT.
LEI ZHANG, TIEFENG XU, XIAOFEN SUN, HANMING ZHANG, TIANLIN TANG, KEXUAN TANG
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Abstract

An efficient in vitro plant regeneration system from cotyledons was established in tetraploid Isatis indigotica Fort. Factors influencing shoot regeneration from cotyledons, including culture medium type, combinations of plant growth regulators, and sucrose concentrations in the medium, as well as illumination were investigated. Murashige and Skoog's (MS) medium was found to be best for promoting shoot regeneration, followed by Gamborg's B5 and White's medium. The highest shoot regeneration frequency was achieved from cotyledons cultured on MS medium supplemented with 2.0 mg l−1 (8.9 μM) 6-benzyladenine and 1.0 mg l−1 (5.4 μM) α-naphthaleneacetic acid (NAA), with 97.9% regeneration, associated with a high number of multiple shoots developed per explant (8.6 shoots per explant). A sucrose concentration of 3% present in the medium and light conditions were beneficial for shoot regeneration. The shoots developed were rooted in a half-strength MS medium supplemented with 1.0 mg l−1 (5.4 μM) NAA and successfully transplanted in soil in pots with over 85% survival. The establishment of an efficient plant regeneration procedure from cotyledons provides a basis for the rapid in vitro multiplication of tetraploid Isatis indigotica Fort., one of the most extensively used medicinal plants in China currently under great shortage.

LEI ZHANG, TIEFENG XU, XIAOFEN SUN, HANMING ZHANG, TIANLIN TANG, and KEXUAN TANG "FACTORS INFLUENCING SHOOT REGENERATION FROM COTYLEDONS OF TETRAPLOID ISATIS INDIGOTICA FORT.," In Vitro Cellular and Developmental Biology - Plant 39(5), 459-462, (1 September 2003). https://doi.org/10.1079/IVP2003447
Received: 17 July 2002; Accepted: 1 January 2003; Published: 1 September 2003
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KEYWORDS
Cotyledon
Plant regeneration
tetraploid Isatis indigotica Fort
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