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1 September 2003 INFLUENCE OF PHENYLACETIC ACID ON CLONAL PROPAGATION OF DECALEPIS HAMILTONII WIGHT & ARN: AN ENDANGERED SHRUB
P. GIRIDHAR, D. VIJAYA RAMU, B. OBUL REDDY, T. RAJASEKARAN, G. A. RAVISHANKAR
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Abstract

We have developed a highly efficient two-stage protocol for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phenylacetic acid (PAA) had a synergistic effect on shoot multiplication when treated with N6-benzyladenine (BA). This protocol used PAA for both multiple shoot induction from nodal explants, elongation of primary shoots, and initiation of adventitious shoot formation from primary shoots. Murashige and Skoog medium containing BA (2.22–31.08 μM) and α-naphthaleneacetic acid (0.27–10.74 μM) or PAA (7.34–36.71 μM) was used to initiate shoot formation from nodal explants. The maximum number of shoots per culture was produced on a medium containing 31.08 μM BA and 14.68 μM PAA, while the longest shoot length and nodes were obtained on medium containing 22.2 μM BA and 14.68 μM PAA. Shoots subcultured on MS medium containing 22.2 μM BA and 14.68 μM PAA elongated along with secondary shoot formation. The shoots were rooted on medium containing 9.8 μM indole-3-butyric acid. The plantlets were acclimatized in soil with an 80–90% survival rate under field conditions.

P. GIRIDHAR, D. VIJAYA RAMU, B. OBUL REDDY, T. RAJASEKARAN, and G. A. RAVISHANKAR "INFLUENCE OF PHENYLACETIC ACID ON CLONAL PROPAGATION OF DECALEPIS HAMILTONII WIGHT & ARN: AN ENDANGERED SHRUB," In Vitro Cellular and Developmental Biology - Plant 39(5), 463-467, (1 September 2003). https://doi.org/10.1079/IVP2003448
Received: 24 September 2002; Accepted: 1 January 2003; Published: 1 September 2003
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KEYWORDS
axillary bud
explant
Multiple shoots
plantlets
rooting
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