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1 September 2003 PLANT REGENERATION FROM PROTOCORM-DERIVED CALLUS OF CYPRIPEDIUM FORMOSANUM
YUNG-I LEE, NEAN LEE
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Abstract

Totipotent callus of Cypripedium formosanum, an endangered slipper orchid species, was induced from seed-derived protocorm segments on a quarter-strength Murashige and Skoog medium containing 4.52 μM 2,4-dichlorophenoxyacetic acid and 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron). This callus proliferated well and was maintained by subculturing on the same medium. On average, 13 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the medium with 4.44 μM N6-benzyladenine after 8 wk of culture. The regenerated protocorm-like bodies formed shoots and roots on medium containing 1 g l−1 activated charcoal and 20 g l−1 potato homogenate. After 24 wk of culture on this medium, well-developed plantlets ready for potting were established.

YUNG-I LEE and NEAN LEE "PLANT REGENERATION FROM PROTOCORM-DERIVED CALLUS OF CYPRIPEDIUM FORMOSANUM," In Vitro Cellular and Developmental Biology - Plant 39(5), 475-479, (1 September 2003). https://doi.org/10.1079/IVP2003450
Received: 27 September 2002; Accepted: 1 January 2003; Published: 1 September 2003
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KEYWORDS
Callus induction
Cypripedium formosanum
Plant regeneration
protocorm-like body
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