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1 September 2003 IN VITRO ORGANOGENESIS OF PELECYPHORA ASELLIFORMIS ERHENBERG (CACTACEAE)
M. A R. I A D. E. L S. OCORRO. SANTOS-DÍAZ, R. I C. A R. D. O. MÉNDEZ-ONTIVEROS, A. L B. E R. T. O. ARREDONDO-GÓMEZ, M. A R. I A D. E L. O. URDES. SANTOS-DÍAZ
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Abstract

In vitro propagation of Pelecyphora aselliformis, a Mexican cactus which is considered rare and is highly valued in the commercial market, was initiated using seeds as explants. The longitudinal explants from seedlings germinated in vitro were cultivated on Murashige and Skoog medium containing 8.8 μM benzyladenine (BA) or 4.6 μM kinetin at pH 7.0. After 120 d, each explant gave rise to five shoots and this number of shoots increased 20–25% after subculture. The hyperhydricity was similar in both media, but callus formation was lower on the medium with BA. The shoot development, in terms of epicotyl length, and fresh and dry weight after 6 wk, was also recorded. The epicotyl length was similar on shoot-forming media but the quality of shoots was better on media containing BA. In about 1 yr, 500-600 well-defined shoots were obtained. The rooting of shoots was very slow and a vigorous radical system was observed after 1 yr of culture.

M. A R. I A D. E. L S. OCORRO. SANTOS-DÍAZ, R. I C. A R. D. O. MÉNDEZ-ONTIVEROS, A. L B. E R. T. O. ARREDONDO-GÓMEZ, and M. A R. I A D. E L. O. URDES. SANTOS-DÍAZ "IN VITRO ORGANOGENESIS OF PELECYPHORA ASELLIFORMIS ERHENBERG (CACTACEAE)," In Vitro Cellular and Developmental Biology - Plant 39(5), 480-484, (1 September 2003). https://doi.org/10.1079/IVP2003456
Received: 2 August 2002; Accepted: 1 March 2003; Published: 1 September 2003
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KEYWORDS
cactus
in vitro culture
Pelecyphora
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