Translator Disclaimer
1 September 2003 DIRECT SHOOT REGENERATION FROM LEAF SEGMENTS OF MATURE PLANTS OF ECHINACEA PURPUREA (L.) MOENCH
Author Affiliations +
Abstract

This is the first communication of direct shoot regeneration from fully developed leaves of potted mature Echinacea purpurea plants. Shoot buds were induced directly on the adaxial surface of mature leaf tissues of E. purpurea 30 d after culture initiation on Woody Plant Medium (WPM) supplemented with various levels of 6-benzyladenine (BA). Maximum shoot organogenesis, with 12–20 shoots per leaf segment, was obtained with 5% coconut milk and 2.5 mg l−1 (6 μM) BA in 30 d. Callus was induced using 0.5 mg l−1 (1 μM) α-naphthaleneacetic acid and 2.5 mg l−1 (6 μM) BA. The regenerated shoots were rooted on WPM supplemented with 1.5 mg l−1 (3 μM) of indole-3-butyric acid, 3% sucrose, and 0.85% agarose. Rooted plants were successfully transferred to soil in pots and appeared morphologically normal and flowered in a growth chamber.

SUBBAIAH M. MECHANDA, BERNARD R. BAUM, DOUGLAS A. JOHNSON, and JOHN T. ARNASON "DIRECT SHOOT REGENERATION FROM LEAF SEGMENTS OF MATURE PLANTS OF ECHINACEA PURPUREA (L.) MOENCH," In Vitro Cellular and Developmental Biology - Plant 39(5), 505-509, (1 September 2003). https://doi.org/10.1079/IVP2003461
Received: 2 October 2002; Accepted: 1 January 2003; Published: 1 September 2003
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top