Translator Disclaimer
1 September 2003 IN VITRO PROPAGATION OF THE RARE MEDICINAL PLANT CEROPEGIA CANDELABRUM L. THROUGH SOMATIC EMBRYOGENESIS
M. R. BEENA, K. P. MARTIN
Author Affiliations +
Abstract

Efficient in vitro propagation of Ceropegia candelabrum L. (Asclepidaceae) through somatic embryogenesis was established. Somatic embryogenesis depended on the type of plant growth regulators in the callus-inducing medium. Friable callus, developed from leaf and internode explants grown on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D), underwent somatic embryogenesis. Compared to solid media, suspension culture was superior and gave rise to a higher number of somatic embryos. Transfer of the friable callus developed on MS medium containing 4.52 μM 2,4-D to suspension cultures of half- or quarter-strength MS medium with lower levels of 2,4-D (0.23 or 0.45 μM) induced the highest number of somatic embryos, which developed up to the torpedo stage. Somatic embryogenesis was asynchronous with the dominance of globular embryos. About 100 mg of callus induced more than 500 embryos. Upon transfer to quarter-strength MS agar medium without growth regulators, 50% of the somatic embryos underwent maturation and developed into plantlets. Plantlets acclimatized under field conditions with 90% survival.

M. R. BEENA and K. P. MARTIN "IN VITRO PROPAGATION OF THE RARE MEDICINAL PLANT CEROPEGIA CANDELABRUM L. THROUGH SOMATIC EMBRYOGENESIS," In Vitro Cellular and Developmental Biology - Plant 39(5), 510-513, (1 September 2003). https://doi.org/10.1079/IVP2003468
Received: 16 July 2002; Accepted: 1 May 2003; Published: 1 September 2003
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top