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1 November 2003 IN VITRO REGENERATION OF ECHINACEA PURPUREA L.: ENHANCEMENT OF SOMATIC EMBRYOGENESIS BY INDOLEBUTYRIC ACID AND DARK PRE-INCUBATION
S. M. A. ZOBAYED, P. K. SAXENA
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Abstract

The embryogenic potential of different Echinacea purpurea tissues, viz. leaf, cotyledon, and root, was investigated. Maximum embryo-induction was achieved from leaf discs cultured on Murashige and Skoog medium supplemented with benzylaminopurine (5.0 μM) and indolebutyric acid (2.5 μM) where 95% of the explants responded, yielding an average of 83 embryos per explant within 4 wk of culture. Incubation of cultures in the dark for an initial period of 14 d significantly increased the frequency of somatic embryogenesis (6–8-fold in leaf explants). Exposure of the abaxial surface of leaves to the medium significantly increased the number of embryos. Transfer of somatic embryos to a medium devoid of growth regulators resulted in 80% germination within 7 d. Over 73% of the somatic embryos developed roots within 28 d of culture on a medium containing naphthaleneacetic acid (10 μM) with a maximum root number of 9.8 per plantlet. Transplanting ex vitro and acclimatization for a period of 7 d were sufficient to promote establishment of plants in the greenhouse, and more than 90% of the regenerated plants survived.

S. M. A. ZOBAYED and P. K. SAXENA "IN VITRO REGENERATION OF ECHINACEA PURPUREA L.: ENHANCEMENT OF SOMATIC EMBRYOGENESIS BY INDOLEBUTYRIC ACID AND DARK PRE-INCUBATION," In Vitro Cellular and Developmental Biology - Plant 39(6), 605-612, (1 November 2003). https://doi.org/10.1079/IVP2003434
Received: 4 November 2002; Accepted: 1 April 2003; Published: 1 November 2003
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KEYWORDS
Echinacea purpurea
Medicinal plant
micropropagation
regeneration
somatic embryogenesis
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