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1 March 2004 IN VITRO SHOOT REGENERATION FROM COTYLEDONARY NODE EXPLANTS OF A MULTIPURPOSE LEGUMINOUS TREE, PTEROCARPUS MARSUPIUM ROXB.
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Abstract

A protocol has been developed for in vitro plant regeneration from cotyledonary nodes of Pterocarpus marsupium Roxb. Multiple shoots were induced from cotyledonary nodes derived from 20-d-old axenic seedlings grown on Murashige and Skoog (MS) medium containing 2.22–13.32 μM benzyladenine (BA) or 2.32–13.93 μM kinetin alone or in combination with 0.26 μM α-naphthaleneacetic acid (NAA). The highest frequency for shoot regeneration (85%) and maximum number of shoots per explant (9.5) were obtained on the medium supplemented with 4.44 μM BA and 0.26 μM NAA after 15 wk of culture. A proliferating shoot culture was established by repeatedly subculturing the original cotyledonary nodal explant on fresh medium after each harvest of the newly formed shoots. Nearly 30% of the shoots formed roots after being transferred to half-strength MS medium containing 9.84 μM indole-3-butyric acid (IBA) after 25 d of culture. Fifty percent of shoots were also directly rooted as microcuttings on a peat moss, soil, and compost mixture (1:1:1). About 52% of plantlets were successfully acclimatized and established in pots.

SURESH CHAND and AJAY KUMAR SINGH "IN VITRO SHOOT REGENERATION FROM COTYLEDONARY NODE EXPLANTS OF A MULTIPURPOSE LEGUMINOUS TREE, PTEROCARPUS MARSUPIUM ROXB.," In Vitro Cellular and Developmental Biology - Plant 40(2), 167-170, (1 March 2004). https://doi.org/10.1079/IVP2003488
Received: 28 March 2003; Published: 1 March 2004
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