Translator Disclaimer
1 March 2004 DIRECT SHOOT REGENERATION FROM LEAF EXPLANTS OF ROSA DAMASCENA MILL.
Author Affiliations +
Abstract

A protocol for in vitro propagation using direct induction of shoot buds from leaf explants of in vitro-raised shoots of Rosa damascena var. Jwala is reported. The present study is the first report on direct shoot regeneration in scented roses. Elite plants raised from nodal explants and maintained for over 2 yr in vitro on a static liquid shoot multiplication Murashige and Skoog (MS) medium supplemented with 5.0 μM benzyladenine (BA) and 3% sucrose were used. Petioles from fully developed young leaves, obtained after 4 wk of pruning of old shoots, were found to be ideal for regeneration of shoots. Initially the explants were cultured in an induction medium [half-strength MS 3%sucrose 6.8 μM thidiazuron 0.27 μM α-naphthaleneacetic acid (NAA) 17.7 μM AgNO3] and subsequently transferred to the regeneration medium (MS 2.25 μM BA 0.054 μM NAA) after 7, 14, 21, 28, and 35 d. The highest shoot regeneration response (69%) was recorded when shoots were kept in the induction medium for 21 d and later transferred to regeneration medium. Histological studies revealed direct formation of shoot buds without the intervening callus phase. In vitro rooting of micro-shoots was accomplished within 2 wk on half-strength MS liquid medium supplemented with 10.0 μM IBA and 3% sucrose for 1 wk in the dark and later transferred to hormone-free medium and kept in the light. Plantlets, remaining in the latter medium for 5–6 wk when transferred to soil, showed 90% survival.

PRATAP KUMAR PATI, MADHU SHARMA, ANIL SOOD, and P. S. AHUJA "DIRECT SHOOT REGENERATION FROM LEAF EXPLANTS OF ROSA DAMASCENA MILL.," In Vitro Cellular and Developmental Biology - Plant 40(2), 192-195, (1 March 2004). https://doi.org/10.1079/IVP2003503
Received: 7 May 2003; Accepted: 1 September 2003; Published: 1 March 2004
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top