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1 March 2004 DIRECT SOMATIC EMBRYOGENESIS INDUCED FROM COTYLEDONS OF MANGO IMMATURE ZYGOTIC EMBRYOS
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Abstract

For the first time, regenerated plantlets were obtained from immature zygotic embryos of mango (Mangifera indica L.) through direct somatic embryogenesis. Pro-embryogenic mass (PEM)-like structures, which are differentiated as clusters of globular structures, were easily induced directly from the abaxial side of cotyledons from immature fruits, 2.0–3.5 cm diameter by a 2-wk culture period on a modified Murashige and Skoog medium with 5 mg l−1 (25 μM) indole-3-butyric acid (IBA). Conversion of somatic embryos into plantlets was achieved after 4 wk of culture on the conversion medium containing 5 mg l−1 (23 μM) kinetin. Secondary somatic embryogenesis could also be obtained directly from the hypocotyls of mature primary somatic embryos cultured on the conversion medium. In our experimental system, only minor problems were noted with browning of cultures.

JIE-NING XIAO, XUE-LIN HUANG, YONG-JIE WU, XIAO-JU LI, MING-DE ZHOU, and FLORENT ENGELMANN "DIRECT SOMATIC EMBRYOGENESIS INDUCED FROM COTYLEDONS OF MANGO IMMATURE ZYGOTIC EMBRYOS," In Vitro Cellular and Developmental Biology - Plant 40(2), 196-199, (1 March 2004). https://doi.org/10.1079/IVP2003513
Received: 3 June 2003; Accepted: 1 October 2003; Published: 1 March 2004
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