Black grama (Bouteloua eriopoda) is an important forage grass in southwestern USA rangelands. Plants were regenerated by somatic embryogenesis. Surface-disinfested seeds were germinated and the embryonic shoots were excised and cultured on Murashige and Skoog (MS) medium gelled with agar. Callus was induced from apical meristems. Calluses were cultured on MS solid medium with six concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or Dicamba (6-dichloro-o-anisic acid) for 6 wk under light or dark conditions. Somatic embryo induction was greatest on 4.52 μM Dicamba, under light, after transferring to an auxin-free medium. Embryo development progressed from globular torpedo to mature embryos phenotypically identical to those naturally produced in seed. These germinated and grew into intact plants and were established in soil and grown to maturity. To our knowledge, this is the first report of somatic embryo induction and regeneration in black grama grass.
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Vol. 40 • No. 3