How to translate text using browser tools
1 July 2004 CRYOPRESERVATION OF AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.) SHOOT TIPS
ASMARA D. MOGES, RIDA A. SHIBLI, NABILA S. KARAM
Author Affiliations +
Abstract

Cryopreservation of African violet via encapsulation–dehydration, vitrification, and encapsulation–vitrification of shoot tips was evaluated. Encapsulation–dehydration, pretreatment of shoot tips with 0.3 M sucrose for 2 d followed by air dehydration for 2 and 4 h resulted in complete survival and 75% regrowth, respectively. Dehydration of encapsulated shoot tips with silica gel for 1 h resulted in 80% survival but only 30% regrowth. Higher viability of shoot tips was obtained when using a step-wise dehydration of the material rather than direct exposure to 100% plant vitrification solution (PVS2). Complete survival and 90% regrowth were achieved with a four-step dehydration with PVS2 at 25°C for 20 min prior to freezing. The use of 2 M glycerol plus 0.4 M sucrose or 10% dimethyl sulfoxide (DMSO) plus 0.5 M sucrose as a cryoprotectant resulted in 55% survival of shoots. The greatest survival (80–100%) and regrowth (80%) was obtained when shoot tips were cryoprotected with 10% DMSO plus 0.5 M sucrose or 5% DMSO plus 0.75 M sucrose followed by dehydration with 100% PVS2. Shoot tips cryoprotected with 2 M glycerol plus 0.4 M sucrose for 20 min exhibited complete survival (100%) and the highest regrowth (55%). In encapsulation–vitrification, dehydration of encapsulated and cryoprotected shoot tips with 100% PVS2 at 25°C for 5 min resulted in 85% survival and 80% regrowth.

ASMARA D. MOGES, RIDA A. SHIBLI, and NABILA S. KARAM "CRYOPRESERVATION OF AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.) SHOOT TIPS," In Vitro Cellular and Developmental Biology - Plant 40(4), 389-395, (1 July 2004). https://doi.org/10.1079/IVP2004536
Received: 6 May 2003; Accepted: 1 January 2004; Published: 1 July 2004
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

KEYWORDS
African violet
cryopreservation
encapsulation
preservation
vitrification
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top