Multiple shoots were induced from cotyledonary nodes of grasspea (Lathyrus sativus L.) derived from 7-d-old in vitro seedlings on Murashige and Skoog (MS) medium containing N6-benzyladenine (BA), kinetin, or thidiazuron, BA being the most effective. Among the five genotypes tested, shoot proliferation frequency was the highest (93.3%) for IC-120487, giving the maximum number of shoots (11.3 shoots per explant) on MS medium augmented with 2.0 mg l−1 (8.87 μM) BA. Shoot cultures were established by repeatedly subculturing the original cotyledonary nodes on fresh medium after each harvest of the newly formed shoots. Thus 30–40 shoots were obtained in 2 mo. from a single cotyledonary node. Up to 81.8% of the shoots developed roots following transfer to half-strength MS medium containing 0.5 mg l−1 (2.85 μM) indole-3-acetic acid. Plantlets were successfully acclimatized and established in soil.
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1 September 2004
HIGH-FREQUENCY PLANT REGENERATION BY IN VITRO SHOOT PROLIFERATION IN COTYLEDONARY NODE EXPLANTS OF GRASSPEA (LATHYRUS SATIVUS L)
D. P. BARIK,
S. K. NAIK,
U. MOHAPATRA,
P. K. CHAND
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In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 5
September 2004
Vol. 40 • No. 5
September 2004
Cotyledonary node
grain legumes
grasspea
Lathyrus sativus L
Plant regeneration