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1 November 2004 IN VITRO REGENERATION OF SALVIA NEMOROSA L. FROM SHOOT TIPS AND LEAF EXPLANTS
E. W A. SKAłA, H. A L. I N. A. WYSOKIŃSKA
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Abstract

Shoot tips and leaves excised from in vitro shoot cultures of Salvia nemorosa were evaluated for their organogenic capacity under in vitro conditions. The best shoot proliferation from shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with 8.9 μM 6-benzylaminopurine (BA) and 2.9 μM indole-3-acetic acid (IAA). Leaf lamina and petiole explants formed shoots through organogenesis via callus stage and/or directly from explant tissue. The highest values for shoot regeneration were obtained with 0.9 μM BA and 2.9 μM IAA for lamina explants. No shoot organogenesis was obtained on leaf explants cultured on MS medium supplemented with α-naphthaleneacetic acid (NAA). The regenerated shoots rooted the best on MS medium containing 0.6 μM IAA or 0.5 μM NAA. In vitro-propagated plants were transferred to soil with a survival rate of 85% after 3 mo.

E. W A. SKAłA and H. A L. I N. A. WYSOKIŃSKA "IN VITRO REGENERATION OF SALVIA NEMOROSA L. FROM SHOOT TIPS AND LEAF EXPLANTS," In Vitro Cellular and Developmental Biology - Plant 40(6), 596-602, (1 November 2004). https://doi.org/10.1079/IVP2004580
Received: 23 February 2004; Accepted: 1 July 2004; Published: 1 November 2004
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