Translator Disclaimer
1 January 2005 A SIMPLE WHEAT HAPLOID AND DOUBLED HAPLOID PRODUCTION SYSTEM USING ANTHER CULTURE
KYUNG-MOON KIM, P. STEPHEN BAENZIGER
Author Affiliations +
Abstract

Wheat (Triticum aestivum L.) haploids and doubled haploids have been used in breeding programs and genetic studies. Wheat haploids and doubled haploids via anther culture are usually produced by a multiple step culture procedure. We improved a wheat haploid and doubled haploid production system via anther culture in which plants are produced from microspore-derived embryos using one medium and one culture environment. In the improved protocol, tillers of donor plants were pretreated at 4°C for 1–2 wk before anthers were plated on a modified 85D12 basal medium with phenylacetic acid (PAA) and zeatin and cultured at 30°C with a 12-h daylength (43 μE s−1 m−2) in an incubator. Microspore-derived embryos developed in 2–3 wk and the plants were produced 3–4 wk after anther plating. In the improved system, as much as 53% of the anthers of Pavon 76 were responsive with multiple embryos. For plant regeneration, as many as 22 green and 25 albino plants were produced from 100 anthers. Sixty-five green plants were grown to maturity and 32 (49%) plants were fertile and produced seeds (indicating spontaneous chromosome doubling) while 33 plants did not produce seed. Of five Nebraska breeding lines tested using the protocol, NE96675 was very responsive and the other lines less so, indicating that the protocol is genotype-dependent.

KYUNG-MOON KIM and P. STEPHEN BAENZIGER "A SIMPLE WHEAT HAPLOID AND DOUBLED HAPLOID PRODUCTION SYSTEM USING ANTHER CULTURE," In Vitro Cellular and Developmental Biology - Plant 41(1), 22-27, (1 January 2005). https://doi.org/10.1079/IVP2004594
Received: 26 June 2003; Accepted: 1 September 2004; Published: 1 January 2005
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

Share
SHARE
KEYWORDS
microspore
one step culture
Plant tissue culture
Triticum aestivum
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top