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1 January 2005 EFFECTS OF THE GROWTH RETARDANT PACLOBUTRAZOL ON LARGE-SCALE MICROPROPAGATION OF DAYLILY (HEMEROCALLIS SPP.)
JIANXIN CHEN, DAWN E. HALL, VINCENZO DE LUCA
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Abstract

Meristematic clusters were induced from daylily scape explants (pedicel–scape junction) in the presence of the growth retardant Paclobutrazol on semisolid agar medium. Liquid shake culture was used to proliferate meristematic clusters. Highly efficient regeneration of adventitious shoots occurred on clusters after subculture on a 0.8% agar strength semisolid medium with the addition of activated charcoal. Paclobutrazol and sucrose levels in the media were found to significantly affect starch accumulation, growth value, and dry weight percentage of liquid-cultured meristematic clusters. The use of liquid shake cultures for mass proliferation of meristematic clusters followed by regeneration of adventitious shoots on semisolid agar culture could be an efficient system for large-scale micropropagation of daylily.

JIANXIN CHEN, DAWN E. HALL, and VINCENZO DE LUCA "EFFECTS OF THE GROWTH RETARDANT PACLOBUTRAZOL ON LARGE-SCALE MICROPROPAGATION OF DAYLILY (HEMEROCALLIS SPP.)," In Vitro Cellular and Developmental Biology - Plant 41(1), 58-62, (1 January 2005). https://doi.org/10.1079/IVP2004595
Received: 6 April 2004; Accepted: 1 September 2004; Published: 1 January 2005
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KEYWORDS
daylily
growth retardant
Hemerocallis
Liquid culture
micropropagation
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