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1 January 2005 IN VITRO SHOOT MULTIPLICATION THROUGH SHOOT TIP CULTURES OF DECALEPIS HAMILTONII WIGHT & ARN., A THREATENED PLANT ENDEMIC TO SOUTHERN INDIA
P. GIRIDHAR, H. B. GURURAJ, G. A. RAVISHANKAR
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Abstract

An efficient and rapid micropropagation system was developed for a food and medicinally important endangered shrub, Decalepis hamiltonii (‘swallow root’), through shoot multiplication. The influence of 2.5–7.5 μM isopentenyladenine (2iP), 4.4–17.7 μM 6-benzyladenine, 2.3–4.7 μM kinetin, 2.8–6.8 μM thidiazuron, and 2.3–11.4 μM zeatin alone and in combination with 0.3–0.9 μM indole-3-acetic acid (IAA) on in vitro multiple shoot production was studied. The maximum number of multiple shoots (6.5 ± 0.4) was induced from shoot tips cultured on agar-based Murashige and Skoog (MS) medium containing 4.9 μM 2iP. But, both zeatin (9.1 μM) and kinetin (4.7 μM) in combination with IAA (0.6 μM) were able to produce a maximum of 5.0 ± 0.4 and 5.l ± 0.4 multiple shoots, respectively. Further elongation of shoots and adventitious shoot formation was obtained on medium containing 2.5 μM 2iP and 0.3 μM gibberellic acid. Elongated shoots were separated and rooted on MS medium supplemented with 9.8 μM indole-3-butyric acid (IBA) and various phenolic compounds within 5–6 wk. Phloroglucinol and salicylic acid interaction with IBA stimulated in vitro rooting of shoots. Successful field transfer was achieved in rooted plantlets.

P. GIRIDHAR, H. B. GURURAJ, and G. A. RAVISHANKAR "IN VITRO SHOOT MULTIPLICATION THROUGH SHOOT TIP CULTURES OF DECALEPIS HAMILTONII WIGHT & ARN., A THREATENED PLANT ENDEMIC TO SOUTHERN INDIA," In Vitro Cellular and Developmental Biology - Plant 41(1), 77-80, (1 January 2005). https://doi.org/10.1079/IVP2004600
Received: 16 December 2003; Accepted: 1 September 2004; Published: 1 January 2005
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KEYWORDS
kinetin
rooting
Shoot multiplication
shoot tips
Zeatin
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