Translator Disclaimer
1 January 2005 THE EFFECT OF PHYTOHORMONES ON GROWTH AND ARTEMISININ PRODUCTION IN ARTEMISIA ANNUA HAIRY ROOTS
P. J. WEATHERS, G. BUNK, M. C. McCOY
Author Affiliations +
Abstract

Few studies have focused on the effect of a broad range of phytohormones on growth and secondary metabolism of a single hairy root species. We measured growth, development, and production of the antimalarial drug, artemisinin, in Artemisia annua hairy roots in response to the five main hormones: auxins, cytokinins, ethylene, gibberellins (GA), and abscisic acid (ABA). Single roots grown in six-well plates in medium B5 with 0.01 mg l−1 (0.029 μM) GA3 produced the highest values overall in terms of the number of lateral roots, length of the primary root, lateral root tip density, total lateral root length, and total root length. When the total root lengths are compared, the best conditions for stimulating elongation appear to be: GA 0.01 mg l−1 (0.029 μM) > ABA 1.0 mg l−1 (3.78 μM)=GA 0.02 mg l−1 (0.058 μM). Bulk yields of biomass were inversely proportional to the concentration of each hormone tested. All cultures provided with ABA yielded the highest amount of biomass. Both 6-benzylaminopurine and 2-isopentenyladenine inhibited root growth, however, only 2-isopentenyladenine stimulated artemisinin production, more than twice that of the B5 controls, and more than any other hormone studied. These results will prove useful in increasing hairy root growth and artemisinin production.

P. J. WEATHERS, G. BUNK, and M. C. McCOY "THE EFFECT OF PHYTOHORMONES ON GROWTH AND ARTEMISININ PRODUCTION IN ARTEMISIA ANNUA HAIRY ROOTS," In Vitro Cellular and Developmental Biology - Plant 41(1), 47-53, (1 January 2005). https://doi.org/10.1079/IVP2004604
Received: 9 April 2004; Accepted: 1 September 2004; Published: 1 January 2005
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

Share
SHARE
KEYWORDS
Artemisia annua
terpenoid
transformed roots
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top